Elucidation of the biosynthetic and action mechanisms of novel bacteriocins with characteristic structure and activity from lactic acid bacteria and their applications
| Project/Area Number |
17H03797
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|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
Zendo Takeshi 九州大学, 農学研究院, 助教 (50380556)
|
|---|
| Project Period (FY) |
2017-04-01 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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|---|
| Keywords | バクテリオシン / 抗菌ペプチド / 乳酸菌 / 生合成機構 |
|---|
| Outline of Final Research Achievements |
Bacteriocins were discovered from newly isolated lactic acid bacteria (LAB), and some of them were identified as novel bacteriocins by structural analysis of purified bacteriocins and draft genome analysis of the producer LAB isolates. Among the various bacteriocins we previously discovered and identified, some circular bacteriocins were analyzed for their biosynthetic genes and proteins, and their mechanisms for self-immunity, secretion, and circularization were characterized. A multiple bacteriocin transporter was characterized for the substrate specificity, and its availability for secretion of various bacteriocins was demonstrated. A species-specific bacteriocin was characterized for the biosynthesis and action mechanisms.
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| Academic Significance and Societal Importance of the Research Achievements |
安全な抗菌物質としての利用が期待される乳酸菌バクテリオシンについて、新しい構造や活性もつものを多数見出し、それらが有する特徴的な生合成機構や作用機構の一端を明らかにした。これらを基盤とすることで、用途に応じた乳酸菌バクテリオシンの選択やより安全な利用が可能になる。さらには、新奇乳酸菌バクテリオシンの構造と環状化機構などの新しい生合成機構を利用した抗菌ペプチドの設計や高生産への展開が期待される。
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Report
(4 results)
Research Products
(49 results)
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[Book] 発酵美容成分の開発2020
Author(s)
小山 遼、善藤威史、園元謙二（監修： 尾関健二）
Total Pages
197
Publisher
シーエムシー出版
ISBN
9784781314990
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[Book] 腸内細菌の応用と市場2018
Author(s)
佐戸 翔太, 善藤 威史, 園元 謙二
Total Pages
345
Publisher
シーエムシー出版
ISBN
9784781313245
Related Report
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