Light regulation mechanism of ascorbate biosynthesis in plants
| Project/Area Number |
17H03807
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
丸田 隆典 島根大学, 学術研究院農生命科学系, 准教授 (50607439)
吉村 和也 中部大学, 応用生物学部, 准教授 (90379561)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Keywords | ビタミンC / シロイヌナズナ / 生合成 / 光調節 / アスコルビン酸生合成 / 光情報伝達 / 遺伝子発現 / リン酸化修飾 |
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| Outline of Final Research Achievements |
Plants have a great ability to synthesis ascorbate known as vitamin C in response to light. In this study, to understand the molecular mechanism of light-inducible ascorbate biosynthesis, we carried out gene and protein expression analysis and obtained the following conclusion. 1) We generate novel Arabidopsis mutants showing abnormal VTC2 gene expression pattern in response to light, and analyzed their causal genes. 2) One of key regulation of light-inducible ascorbate biosynthesis is phosphorylation of some proteins via VTC3, a novel enzyme, and its down-stream target factor would be VTC2. Actually, recombinant VTC2 analysis showed that phosphorylation of affect significantly its enzymatic stability.
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| Academic Significance and Societal Importance of the Research Achievements |
アスコルビン酸は植物細胞内で最も高濃度に存在する水溶性酸化還元物質であり、環境応答やさまざまな生理作用に関連している。したがってアスコルビン酸生合成調節機構の解明は、環境ストレス応答に関わる細胞内レドックス制御機構の解明をはじめ、植物が持つさまざま生理現象の理解に向けて学術的に大きな意義がある。また本研究で得られた知見は、植物がアスコルビン酸合成能力を高め維持するための環境条件など外部要因の的確な理解につながり、より好条件での作物栽培や育種あるいは収穫後の保蔵に関する有益な知見が得られることや、高付加価値を持つ高ビタミンC含有作物の開発や有用植物の育成法への応用が期待できる。
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Report
(5 results)
Research Products
(28 results)
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[Journal Article] Chloroplast development activates the expression of ascorbate biosynthesis-associated genes in Arabidopsis roots.2019
Author(s)
Shiroma, S., Tanaka, M., Sasaki, T., Ogawa, T., Yoshimura, K., Sawa, Y., Maruta, T., Ishikawa, T.
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Journal Title
Plant Science
Volume: 284
Pages: 185-191
DOI
Related Report
Peer Reviewed
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