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Can high tolerance of Cryptomeria japonica to ozone function effectively to prolonged drought?

Research Project

Project/Area Number 17H03839
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Forest science
Research InstitutionForest Research and Management Organization

Principal Investigator

TOBITA Hiroyuki  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (10353781)

Co-Investigator(Kenkyū-buntansha) 伊豆田 猛  東京農工大学, (連合)農学研究科(研究院), 教授 (20212946)
矢崎 健一  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (30353890)
深山 貴文  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (40353875)
上野 真義  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (40414479)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords対流圏オゾン / 天然スギ / バイオマス配分 / モノテルペン / 活性酸素消去系酵素活性 / 遺伝子発現 / 揮発性有機物 / トランスクリプトーム解析 / 林学 / 気候変動 / ストレス / 生理活性 / 遺伝子
Outline of Final Research Achievements

We examined the effects of elevated O3 concentrations on photosynthesis, growth, monoterpene emission characteristics, activity of radical scavenging enzymes, the concentration of antioxidants, and gene expression of three major gene pools of Cryptomeria japonica clones in a free-air concentration enrichment system. The seedlings were exposed to ambient air (control) and elevated O3 (double the control) for two growing seasons. Total plant biomass as well as biomass allocation to the roots exhibited no change by elevated O3. The responses in monoterpene emission and the concentration of antioxidants to elevated O3 showed significant difference among clones, seasons, and leaf ages. These results mean there are variations in tolerance mechanisms among three clones to elevated O3. In the second experiment of O3 exposure and drought by interception of rainfall, the suppression of growth in C. japonica seedlings by soil drought was suggested to be alleviated under elevated O3.

Academic Significance and Societal Importance of the Research Achievements

オゾン付加実験によりオゾン耐性機構の一つと考えられるモノテルペンの放出量にスギの系統間差があることを2本の論文により公表することができた。遺伝子情報の解析が進んでいる天然スギを材料に用いたことにより、これまで未確認であったオゾン付加によるストレス応答に関連する遺伝子発現の誘導を明らかにすることができた。本課題の成果は、将来の大気中のオゾン濃度変化に対するスギの成長反応を予測し、炭素固定量を予測する際に役立ち、農林水産省の地球温暖化対策に貢献する。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (8 results)

All 2020 2019 2018 2017

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Seasonal Changes in Interclone Variation Following Ozone Exposure on Three Major Gene Pools: An Analysis of Cryptomeria Japonica Clones2019

    • Author(s)
      Miyama Takafumi、Tobita Hiroyuki、Uchiyama Kentaro、Yazaki Kenichi、Ueno Saneyoshi、Uemura Akira、Matsumoto Asako、Kitao Mitsutoshi、Izuta Takeshi
    • Journal Title

      Atmosphere

      Volume: 10 Issue: 11 Pages: 643-643

    • DOI

      10.3390/atmos10110643

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of Combined CO2 and O3 Exposures on Net CO2 Assimilation and Biomass Allocation in Seedlings of the Late-Successional Fagus Crenata2019

    • Author(s)
      Tobita、Komatsu、Harayama、Yazaki、Kitaoka、Kitao
    • Journal Title

      Climate

      Volume: 7 Issue: 10 Pages: 117-117

    • DOI

      10.3390/cli7100117

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differences in monoterpene emission characteristics after ozone exposure between three clones representing major gene pools of <I>Cryptomeria japonica</I>2018

    • Author(s)
      Takafumi Miyama, Hiroyuki Tobita, Kentaro Uchiyama, Kenichi Yazaki, Saneyoshi Ueno, Takami Saito, Asako Matsumoto, Mitsutoshi Kitao, Takeshi Izuta
    • Journal Title

      Journal of Agricultural Meteorology

      Volume: 74 Issue: 3 Pages: 102-108

    • DOI

      10.2480/agrmet.D-17-00043

    • NAID

      130007403783

    • ISSN
      0021-8588, 1881-0136
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 高オゾン下における無降雨期間の長期化がスギの光合成・成長に及ぼす影響2020

    • Author(s)
      飛田博順、上野真義、深山貴文、伊豆田猛、矢崎健一、上村章、松本麻子、内山憲太郎、北尾光俊
    • Organizer
      第67回日本生態学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] オゾン暴露スギ挿し木苗のトランスクリプトーム解析2020

    • Author(s)
      上野真義、飛田博順、深山貴文、伊豆田猛、矢崎健一、内山憲太郎、松本麻子、北尾光俊
    • Organizer
      第67回日本生態学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 系統の異なるスギのモノテルペン放出特性2018

    • Author(s)
      深山貴文、飛田博順
    • Organizer
      第129回日本森林学会大会講演要旨集、166
    • Related Report
      2017 Annual Research Report
  • [Presentation] 遺伝子プールが異なるスギ3品種のモノテルペン放出特性への長期オゾン暴露影響の比較2018

    • Author(s)
      深山貴文、飛田博順、内山憲太郎、矢崎健一、上野真義、齋藤隆実、松本麻子、北尾光俊、伊豆田猛(東京農工大)
    • Organizer
      日本農業気象学会2018年全国大会講演要旨集、198
    • Related Report
      2017 Annual Research Report
  • [Presentation] Effects of soil drought, elevated CO2, and phosphorus deficiency on photoinhibition in Alnus species.(ハンノキ属樹種の光阻害感受性に及ぼす、土壌乾燥、高CO2、リン酸不足の影響)2017

    • Author(s)
      Hiroyuki Tobita(飛田博順), Akira Uemura(上村章), Satoshi Kitaoka(北岡哲), Mitsutoshi Kitao(北尾光俊), Hajime Utsugi(宇都木玄)
    • Organizer
      IUFRO Tokyo 2017 Programme and Abstracts, P87
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2021-02-19  

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