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Foundation of virus oceanography based on comprehensive virome analysis

Research Project

Project/Area Number 17H03850
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic bioproduction science
Research InstitutionKyoto University

Principal Investigator

Yoshida Takashi  京都大学, 農学研究科, 教授 (80305490)

Co-Investigator(Kenkyū-buntansha) 緒方 博之  京都大学, 化学研究所, 教授 (70291432)
中野 伸一  京都大学, 生態学研究センター, 教授 (50270723)
左子 芳彦  京都大学, 農学研究科, 教授 (60153970)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords海洋低次生態系 / ウイルス / 微生物 / メタゲノム / ビローム / 環境ウイルス / 海洋ウイルス / 海洋微生物 / 生物間相互作用 / 海洋メタゲノム / ゲノム
Outline of Final Research Achievements

Marine microbial viruses change the carbon flux through lysis of the host and are involved in the in the global carbon cycle. In this subject, we applied our comprehensive viral metagenomic (virome) analysis method to analyze microorganisms and virus communities at high resolution and build a foundation for understanding a new marine ecosystem. First, we constructed a method to search for viruses infecting a marine dominant lineage Bacteroides from the host-unidentified viral genomes by bioinformatics and found 81 viruses. By applying this method, we analyzed monthly microbial and viral community changes in the Osaka Bay for two years. The potential host-virus interactions showed seasonal variation and were visualized as one-to-many pairs. Furthermore, 13 new freshwater cyanobacterial viruses were found by this method.

Academic Significance and Societal Importance of the Research Achievements

海洋生態系は極めて多様な微生物の代謝過程に支えられている。微生物感染性ウイルスは、宿主微生物の数をはるかに上回ることから、ウイルスごとの宿主微生物、多様性と分布を明らかにすることが、物質循環過程を理解する上で極めて重要である。本研究では包括的メタゲノム手法を確立し、計49属646種について新たにウイルスを同定することに成功した。これらの動態を2年間にわたって調査し、宿主1種に対して複数のウイルス群集が相互作用し、宿主動態に応じて季節変動することを示した。ウイルス-微生物の組み合わせごとに溶菌過程を把握する基礎技術を構築したことで、海洋物質循環過程をより高解像度に理解できることが期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (51 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (10 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 10 results,  Open Access: 9 results) Presentation (35 results) (of which Int'l Joint Research: 9 results,  Invited: 4 results) Book (1 results) Remarks (3 results)

  • [Int'l Joint Research] アリカンテ大学(スペイン)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] リトアニア自然研究センター(リトアニア)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] In silico Prediction of Virus-Host Interactions for Marine Bacteroidetes With the Use of Metagenome-Assembled Genomes2020

    • Author(s)
      Kento Tominaga, Daichi Morimoto, Yosuke Nishimura, Hiroyuki Ogata and Takashi Yoshida
    • Journal Title

      Frontiers in Microbiology

      Volume: 28 Pages: 738-738

    • DOI

      10.3389/fmicb.2020.00738

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Earth Is Small for “Leviathans”: Long Distance Dispersal of Giant Viruses across Aquatic Environments2019

    • Author(s)
      Li Yanze、Endo Hisashi、Gotoh Yasuhiro、Watai Hiroyasu、Ogawa Nana、Blanc-Mathieu Romain、Yoshida Takashi、Ogata Hiroyuki
    • Journal Title

      Microbes and Environments

      Volume: 34 Issue: 3 Pages: 334-339

    • DOI

      10.1264/jsme2.ME19037

    • NAID

      130007711567

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cooccurrence of Broad- and Narrow-Host-Range Viruses Infecting the Bloom-Forming Toxic Cyanobacterium Microcystis aeruginosa2019

    • Author(s)
      Morimoto Daichi、Tominaga Kento、Nishimura Yosuke、Yoshida Naohiro、Kimura Shigeko、Sako Yoshihiko、Yoshida Takashi
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 85 Issue: 18

    • DOI

      10.1128/aem.01170-19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome‐resolved viral and cellular metagenomes revealed potential key virus‐host interactions in a deep freshwater lake2019

    • Author(s)
      Okazaki Yusuke、Nishimura Yosuke、Yoshida Takashi、Ogata Hiroyuki、Nakano Shin‐ichi
    • Journal Title

      Environmental Microbiology

      Volume: 21 Issue: 12 Pages: 4740-4754

    • DOI

      10.1111/1462-2920.14816

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Minimum Information about Uncultivated Virus Genomes (MIUViG): a community consensus on standards and best practices for describing genome sequences from uncultivated viruses.2019

    • Author(s)
      Roux S, Adriaenssens EM, Dutilh BE, Koonin EV, et al
    • Journal Title

      Nat Biotechnol

      Volume: 37 Issue: 1 Pages: 29-37

    • DOI

      10.1038/nbt.4306

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] LexA binds to transcription regulatory site of cell division gene ftsZ in toxic cyanobacterium Microcystis aeruginosa.2018

    • Author(s)
      Takashi Honda., Daichi Morimoto., Yoshihiko Sako and Takashi Yoshida
    • Journal Title

      Marine Biotechnology

      Volume: 20 Issue: 4 Pages: 549-556

    • DOI

      10.1007/s10126-018-9826-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Degenerate PCR primers to reveal the diversity of giant viruses in coastal waters.2018

    • Author(s)
      Li Y, Hingamp P, Watai H, Endo H, Yoshida T, Ogata H.
    • Journal Title

      Viruses

      Volume: 10 Issue: 9 Pages: 496-511

    • DOI

      10.3390/v10090496

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Locality and diel cycling of viral production revealed by a 24 h time course cross-omics analysis in a coastal region of Japan2018

    • Author(s)
      Yoshida Takashi, Nishimura Yosuke, Watai Hiroyasu, Haruki Nana, Morimoto Daichi, Kaneko Hiroto, Honda Takashi, Yamamoto Keigo, Hingamp Pascal, Sako Yoshihiko, Goto Susumu, Ogata Hiroyuki
    • Journal Title

      The ISME Journal

      Volume: 印刷中 Issue: 5 Pages: 1287-1295

    • DOI

      10.1038/s41396-018-0052-x

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transcriptome Analysis of a Bloom-Forming Cyanobacterium Microcystis aeruginosa during Ma-LMM01 Phage Infection2018

    • Author(s)
      Morimoto Daichi, Kimura Shigeko., Sako Yoshihiko., Yoshida Takashi
    • Journal Title

      Fronties in Microbiology

      Volume: 9

    • DOI

      10.3389/fmicb.2018.00002

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Incomplete Selective Sweeps of Microcystis Population Detected by the Leader-End CRISPR Fragment Analysis in a Natural Pond2018

    • Author(s)
      Kimura Shigeko ,Uehara Mika, Morimoto Daichi, Momoko Yamanaka, Sako Yoshihiko., Yoshida Takashi
    • Journal Title

      Fronties in Microbiology

      Volume: 9 Pages: 1-9

    • DOI

      10.3389/fmicb.2018.00425

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ウイルスから海洋微生物構造を知る2019

    • Author(s)
      吉田天士
    • Organizer
      IFOがつなぐ京大微生物学のフロントライン
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Cross-omics解析から明らかになった海洋シアノファージの感染動態2019

    • Author(s)
      吉田天士
    • Organizer
      藍藻の分子生物学2019
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ウイルスから水圏低次生態系構造を知る2019

    • Author(s)
      吉田天士
    • Organizer
      国立環境研究所琵琶湖分室セミナー第32回
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] シアノウイルス-宿主相互作用解明に向けた海洋シアノバクテリアの高純度分手法の確立2019

    • Author(s)
      礒崎 達大、富永 賢人、山本圭吾、左子 芳彦、吉田 天士
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 未分離の海洋性Bacteroidetes門細菌感染ウイルスの生物情報学的探索2019

    • Author(s)
      富永 賢人、緒方 博之、吉田 天士
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 海洋性真核微細藻類が原核生物群集構造に与える影響に関する研究2019

    • Author(s)
      武部 紘明、富永賢人、礒崎達大、山本圭吾、渡邉哲弘、吉田天士
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 大阪湾での経時的動態観測で探る海洋微生物-ウイルス群集相互作用2019

    • Author(s)
      富永 賢人、山本圭吾、緒方 博之、吉田 天士
    • Organizer
      2019年環境ウイルス研究集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] アオコ原因ラン藻Microcystis aeruginosaと相互作用する新規ウイルスの環境動態解析2019

    • Author(s)
      森本大地、吉田尚寛、左子芳彦、吉田天士
    • Organizer
      2019年環境ウイルス研究集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 海洋性微細藻類由来有機物が原核生物群集に与える影響に関する研究2019

    • Author(s)
      武部 紘明、富永賢人、山本圭吾、渡邉哲弘、吉田天士
    • Organizer
      日本水産増殖学会第18回大会・令和元年度日本水産学会近畿支部例会
    • Related Report
      2019 Annual Research Report
  • [Presentation] アオコ原因ラン藻 Microcystis aeruginosa のウイルス感染細胞直接定量2019

    • Author(s)
      中川 哲、森本大地、左子芳彦、吉田天士
    • Organizer
      日本水産増殖学会第18回大会・令和元年度日本水産学会近畿支部例会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 長期観測による海洋シアノバクテリア-ウイルス相互作用の解明2019

    • Author(s)
      富永 賢人、晴気七菜、山本圭吾、綿井 博康、左子 芳彦、緒方 博之、吉田 天士
    • Organizer
      藍藻の分子生物学2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] 微細藻類ブルームを起点とした原核生物群集構造の遷移機構に関する研究2019

    • Author(s)
      武部紘明、富永賢人、山本圭吾、渡邉哲弘、吉田天士
    • Organizer
      令和2年度日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 大阪湾における海洋微生物・ウイルスの群集ダイナミクスと生態学的相互作用解析2019

    • Author(s)
      富永賢人、晴気七菜、藤原健太郎、西村陽介、山本圭吾、綿井博康、左子芳彦、緒方博之、吉田天士
    • Organizer
      第13回日本ゲノム微生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] アオコ原因ラン藻Microcystis aeruginosaと相互作用する新規ウイルスの定量的検出法の確立と環境動態解析2019

    • Author(s)
      森本大地・吉田尚寛・左子芳彦・吉田天士
    • Organizer
      平成31年日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] セルソータ―による高純度細胞分取法を利用した海洋ラン藻の細胞内ウイルス定量法の確立の試み2019

    • Author(s)
      礒﨑達大・富永賢人・山本圭吾・左子芳彦・吉田天士
    • Organizer
      平成31年日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 大阪湾の海洋微生物・ウイルスの生態学的相互作用解析2019

    • Author(s)
      富永賢人・西村陽介・山本圭吾・緒方博之・左子芳彦・吉田天士
    • Organizer
      平成31年日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Baltic sea cyanopages: diversity, distribution and insights into virus-host interaction2018

    • Author(s)
      Sigitas Sulsius, Daichi Morimoto, Kento Surname, Gediminas Alzbutas, Jolita Kuznecova, Eugenijus Simolisnas, Ricardas Palkauskas, Emelie Nilson, Karin Holmfeldt & Takashi Yoshida
    • Organizer
      Aquatic Virus Workshop 9
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Transcriptome analysis of a toxic bloom-forming cyanobacterium Microcystis aeruginosa during Ma-LMM01 phage infection2018

    • Author(s)
      Daichi Morimoto, Kento Tominaga, Yosuke Nishimura, Naohiro Yoshida, Shigeko Kimura, Yoshihiko Sako, Takashi Yoshida
    • Organizer
      Aquatic Virus Workshop 9
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microcystis population dynamics associated with its infectios phages detected by the leader-end CRISPR fragment analysis in a natural pond2018

    • Author(s)
      Kimura, S., M. Uehara, D. Morimoto, M. Yamanaka, Y. Sako and T. Yoshida.
    • Organizer
      Aquatic Virus Workshop 9
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comprehensive viromics uncovered the diverse viral community in a deep freshwater lake.2018

    • Author(s)
      Okazaki Y., Nishimura Y., Ogata H., Yoshida T., Nakano S.
    • Organizer
      ISME17
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 海洋性真核微細藻類が原核生物群集構造に与える影響に関する研究2018

    • Author(s)
      武部 紘明・富永賢人・山本圭吾・左子芳彦・吉田天士
    • Organizer
      平成30年度日本水産学会秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 海洋微生物・ウイルス間相互作用の解明に向けた海洋真核微生物の群集ダイナミクス解析2018

    • Author(s)
      富永賢人・晴気七菜・吉安勇人・綿井博康・山本圭吾・左子芳彦・吉田天士
    • Organizer
      平成30年度日本水産学会秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Natural population dynamics of Microcystis aeruginosa associated with its infectious phages detected by the leader-end CRISPR fragment analysis2018

    • Author(s)
      Shigeko Kimura, Mika Uehara, Daichi Morimoto, Momoko Yamanaka, Yoshihiko Sako and Takashi Yoshida
    • Organizer
      2018環境ウイルス研究集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Cross-Omics analysis of a toxic bloom-forming cyanobacterium Microcystis aeruginosa and its viruses2018

    • Author(s)
      Daichi Morimoto, Kento Tominaga, Shigeko Kimura, Yoshihiko Sako, Takashi Yoshida
    • Organizer
      2018環境ウイルス研究集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Optimization of a metabarcoding method for a major group of giant viruses in the environment2018

    • Author(s)
      Prodinger F., Yanze L., Morimoto D., Omae K., Tominaga K., Gotoh Y., Takano Y., Nagasaki K., Hingamp P., Endo H., Yoshida T., Ogata H
    • Organizer
      2018環境ウイルス研究集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] New lineages of marine Bacteroidetes viruses identified by MAGbased host prediction and their ecological interaction with hosts bacteria2018

    • Author(s)
      Kento Tominaga, Hiroyuki Ogata, Yosihiko Sako, and Takashi Yoshida
    • Organizer
      The 5th International Conference of Members of the Genus Flavobacterium 'Flavobacterium 2018'
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 海洋における原核微生物とウイルスの感染ペア同定に向けた特定細胞集団の高純度回収法2018

    • Author(s)
      礒崎達大・富永賢人・左子芳彦・吉田天士
    • Organizer
      日本水産学会近畿支部 平成30年度後期例会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The cross-omics of marine viruses: ecological perspective2018

    • Author(s)
      Takashi Yoshida
    • Organizer
      第12回日本ゲノム微生物学会年会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 未分離の海洋性バクテロイデス門細菌感染ウイルスゲノムの探索と同定2018

    • Author(s)
      富永賢人、左子芳彦、緒方博之、吉田天士
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アオコ原因ラン藻Microcystis aeruginosaの新規ウイルスの網羅的探索2018

    • Author(s)
      森本大地、吉田尚寛、左子芳彦、吉田天士
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Seasonal dynamics of eukaryotic microbes and prokaryotes in Osaka Bay, Japan2017

    • Author(s)
      Nana Haruki, Hiroyasu Watai, Yuto Yoshiyasu, Kana Sawada, Keigo Yamamoto, Masami Suzuki, Yoshihiko Sako and Yoshida Takashi
    • Organizer
      日本水産学会創立85周年記念国際シンポジウム
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Transcriptome analysis of Microcystis aeruginosa during Ma-LMM01 phage infection2017

    • Author(s)
      Daichi Morimot, Takashi Honda, Mai Nakata, Yoshiko Sako and Takashi Yoshida
    • Organizer
      日本水産学会創立85周年記念国際シンポジウム
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] What does viral metagenomics provide for future marine ecology and resources?2017

    • Author(s)
      Takashi Yoshida
    • Organizer
      日本水産学会創立85周年記念国際シンポジウム
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ウイルス分類ツールViPTreeによる大阪湾における海洋ウイルス群集の季節変動の解析2017

    • Author(s)
      富永賢人、西村陽介、 綿井博康、山本圭吾、晴気七菜、左子芳彦、高野義人、緒方博之、長崎慶三、吉田天士
    • Organizer
      第三回新学術領域「ネオウイルス学」領域班会議
    • Related Report
      2017 Annual Research Report
  • [Presentation] Droplet Digital PCR法を用いた有毒ラン藻Microcystis aeruginosaの直接定量法の確立2017

    • Author(s)
      佐々木彩、森本大地、左子芳彦、吉田天士
    • Organizer
      日本水産学会近畿支部後期例会
    • Related Report
      2017 Annual Research Report
  • [Book] DNA Traffic in the Environment.2019

    • Author(s)
      Yoshida, T., Morimoto, D., Kimura, S.
    • Total Pages
      278
    • Publisher
      Springer Singapore
    • ISBN
      9789811334115
    • Related Report
      2018 Annual Research Report
  • [Remarks] 海洋分子微生物学研究室ホームページ

    • URL

      http://www.microbiology.marine.kais.kyoto-u.ac.jp/index.php?id=1

    • Related Report
      2019 Annual Research Report
  • [Remarks] 海洋分子微生物学研究室ホームページ

    • URL

      http://www.microbiology.marine.kais.kyoto-u.ac.jp/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 海洋分子微生物学分野ホームページ

    • URL

      http://www.microbiology.marine.kais.kyoto-u.ac.jp/

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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