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Origin and evolution of hemiclonal Hexagrammos hybrids elongating the lineage lifetime by host-switching

Research Project

Project/Area Number 17H03856
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic bioproduction science
Research InstitutionHokkaido University

Principal Investigator

MUNEHARA Hiroyuki  北海道大学, 北方生物圏フィールド科学センター, 教授 (80212249)

Co-Investigator(Kenkyū-buntansha) 藤本 貴史  北海道大学, 水産科学研究院, 准教授 (10400003)
荒井 克俊  北海道大学, 水産科学研究院, 特任教授 (00137902)
三木 玲香 (三木玲香)  東京農業大学, 生物資源ゲノム解析センター, 研究員 (30451842)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2017: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords遺伝学 / トランスクリプトーム解析 / 二次的遭遇 / ハイブリドジェネシス / 遺伝学的集団構造 / 系統寿命 / アイナメ属雑種 / 半クローン遺伝子 / 水産学 / ゲノム / 染色体 / 魚類 / 交雑 / 集団構造 / 半クローン / 遺伝学集団構造 / 進化 / クローン
Outline of Final Research Achievements

Unisexual vertebrates (i.e., those produced through clonal or hemiclonal reproduction) are typically incapable of purging deleterious mutations, and as a result, are considered short-lived in evolutionary terms although high increasing rate. However, most of clonal and hemiclonal vertebrates have existed far longer than expected theoretical generation longevities. This study clarified that hemiclonal Hexagrammos hybrids use two-way backcrossing (clonal genomes are returned to the gene pool where they can undergo recombination plays an important role in increasing the genetic variability of the hemiclonal genome and reducing the extinction risk). In this way, hemiclonal lineages may have survived longer than predicted through occasional recombinant generation. In addition, genes inducing hemiclonal reproduction by discarding of paternal genome during oogenesis have been identified by RNA-seq although not yet being completely analyzed.

Academic Significance and Societal Importance of the Research Achievements

地球上には、多くの子孫を残す自然淘汰による進化を通じて様々な生物が生まれてきた。そのひとつがクローンや半クローンで、雌だけで子を残せるため有利なはずである。しかし子孫の遺伝的な多様性を作り出せない欠点があるため、種や系統の寿命が短く、有性生殖に比べて繁栄出来ないと考えられていた。本研究では、交雑起源の海産魚のアイナメ属の半クローンについて、系代飼育と野外調査を実施した集団構造の解析から、有性生殖世代と半クローン世代を非周期的に交互に行うことで長い系統寿命を持つことを明らかにした。生物多様性の新たな側面と希少種の保存に役立つ半クローン遺伝子の特定と発現に関しても部分的に明らかにすることが出来た。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (20 results)

All 2020 2019 2018 2017

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (13 results) (of which Invited: 2 results) Book (1 results)

  • [Journal Article] Dispersion and settlement of two sympatric sculpins of the genus Gymnocanthus2020

    • Author(s)
      Yamazaki Aya、Ogino Akino、Munehara Hiroyuki
    • Journal Title

      Journal of Fish Biology

      Volume: 96 Issue: 4 Pages: 1004-1013

    • DOI

      10.1111/jfb.14291

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unisexual hybrids break through an evolutionary dead end by two‐way backcrossing2020

    • Author(s)
      Suzuki Shota、Miyake Shota、Arai Katsutoshi、Munehara Hiroyuki
    • Journal Title

      Evolution

      Volume: 74 Issue: 2 Pages: 392-403

    • DOI

      10.1111/evo.13903

    • NAID

      120006955196

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein2019

    • Author(s)
      Yamazaki Aya、Nishimiya Yoshiyuki、Tsuda Sakae、Togashi Koji、Munehara Hiroyuki
    • Journal Title

      Biomolecules

      Volume: 9 Issue: 4 Pages: 139-151

    • DOI

      10.3390/biom9040139

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Host selection and ovipositor length in eight sympatric species of sculpins that deposit their eggs into tunicates or sponges2019

    • Author(s)
      Awata Satoshi, Sasaki Haruka, Goto Tomohito, Koya Yasunori, Takeshima Hirohiko, Yamazaki Aya, Munehara Hiroyuki
    • Journal Title

      Marine Biology

      Volume: 166 Issue: 5 Pages: 59

    • DOI

      10.1007/s00227-019-3506-4

    • NAID

      120006847758

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2020094

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Gene expression of antifreeze protein in relation to historical distributions of Myoxocephalus fish species2018

    • Author(s)
      Yamasaki A., Nishimiya Y., Tsuda S., Togashi K., Munehara H.
    • Journal Title

      Marine Biology

      Volume: 165 Issue: 11 Pages: 181-181

    • DOI

      10.1007/s00227-018-3440-x

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Karyological evidence of hybridogenesis in Greenlings (Teleostei: Hexagrammidae)2017

    • Author(s)
      Shota Suzuki, Katsutoshi Arai, Hiroyuki Munehara
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 7 Pages: e0180626-e0180626

    • DOI

      10.1371/journal.pone.0180626

    • NAID

      120006335572

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] クジメ系半クローン雑種由来の野外組み換え雑種の出現2019

    • Author(s)
      鈴木将太・宗原弘幸
    • Organizer
      日本水産学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 非捕獲的手法によるアイナメHexagrammos otakiiなわばり雄の成長と繁殖成功度の経年変化2019

    • Author(s)
      大河内裕典・宗原弘幸・橋爪伸崇・鈴木将太・吉田隼祐・中村陽一
    • Organizer
      日本水産学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 海産の卵寄託魚では宿主の種類やサイズの違いが産卵管形態の種内変異を生み出す2019

    • Author(s)
      安房田智司・五十嵐直・瓜生知史・古屋康則・宗原弘幸
    • Organizer
      日本生態学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] クジメ系半クローン雑種由来の野外組み換え雑種の出現2019

    • Author(s)
      鈴木 将太・宗原 弘幸
    • Organizer
      日本水産学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 非捕獲的手法によるアイナメHexagrammos otakiiなわばり雄の成長と繁殖成功度の経年変化2019

    • Author(s)
      大河内裕典・宗原弘幸・橋爪伸崇・ 鈴木将太・吉田隼祐・中村陽一
    • Organizer
      日本水産学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 海産の卵寄託魚では宿主の種類やサイズの違いが産卵管形態の種内変異を生み出す2019

    • Author(s)
      安房田 智司・五十嵐 直・瓜生 知史・古屋 康則・宗原 弘幸
    • Organizer
      日本生態学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] アイナメ属野外雑種の双方向戻し交配2018

    • Author(s)
      鈴木 将太・三宅 翔太・宗原 弘幸
    • Organizer
      日本魚類学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] RNA-seqによるアイナメ属半クローンの発現変動遺伝子の抽出2018

    • Author(s)
      宗原弘幸・鈴木将太・川原玲香
    • Organizer
      日本魚類学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 半クローン生殖由来のスジアイナメを用いたF1雑種の遺伝様式の検証2017

    • Author(s)
      吉田隼祐・鈴木将太・宗原弘幸
    • Organizer
      日本魚類学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 半クローンの細胞遺伝学2017

    • Author(s)
      鈴木将太・三宅翔太・荒井克俊・藤本貴史・宗原弘幸
    • Organizer
      日本魚類学会シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 人為的環境改変を利用するアイナメの繁殖生態2017

    • Author(s)
      宗原弘幸
    • Organizer
      日本魚類学会シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 築港工事根固め材(網袋)で出来るアイナメの繁殖コロニー (ホッケ資源回復へのヒント)2017

    • Author(s)
      宗原弘幸・鈴木将太・橋爪伸崇・加藤大棋・吉田隼祐・大河内裕典・東村拓志
    • Organizer
      日本水産学会北海道支部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 3Dデジタルカメラを用いたアイナメなわばり雄の非捕獲的全長測定2017

    • Author(s)
      橋爪伸崇・宗原弘幸
    • Organizer
      日本魚類学会
    • Related Report
      2017 Annual Research Report
  • [Book] 北海道の磯魚たちのグレートジャーニー2020

    • Author(s)
      宗原弘幸
    • Total Pages
      128
    • Publisher
      海文堂出版
    • ISBN
      9784303800048
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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