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Development of the fish pathogenic bacterial infection prevention technique utilizing the polysaccharide recognition mechanism derived from pathogenic bacteria

Research Project

Project/Area Number 17H03863
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic bioproduction science
Research InstitutionUniversity of Miyazaki

Principal Investigator

Hikima Jun-ichi  宮崎大学, 農学部, 教授 (70708130)

Co-Investigator(Kenkyū-buntansha) 木下 政人  京都大学, 農学研究科, 助教 (60263125)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2020: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords魚病細菌 / インフラマソーム / ゲノム編集 / メダカ / ヒラメ / LPS 認識 / LPS認識 / 魚病 / 感染症 / 免疫 / LPS / CRISPR/Cas
Outline of Final Research Achievements

In this research project, the development of the prevention method to intracellular bacteria infection was conducted using the lipopolysaccharide (LPS) recognition mechanism of fish. However, the LPS recognition mechanism in fish was greatly different from the mammals, especially for the function of the fish inflammasome-related molecules, which we focused on this research project. Interestingly, the analyses using knockout Japanese medaka of inflammasome-related genes (i.e., Casp1 and ASC), which we established in this project, elucidated the importance to induce inflammatory responses and proptosis (inflammatory cell death) as defense mechanism for the infection of not only Edwardsiella piscicida (intracellular bacteria) but also Aeromonas hydrophila.

Academic Significance and Societal Importance of the Research Achievements

魚類のエドワジエラ症は細胞内寄生細菌による感染症のため、効果的な疾病対策が困難とされてきた。その中でリポ多糖(LPS)の免疫賦活効果に着目し、インフラマソーム関連分子の機能について解析してきた。今回解析したメダカにおいて、インフラマソーム関連分子がエドワジエラ症に対して重要な生体防御能を示したことは、今後の本感染症対策法の開発に重要な発見である。また、哺乳類同様、魚類においてASCやCasp1によりパイロトーシスが誘導されることを解明した事実は、分子進化的検知から見ても今後の細胞死研究にとって非常に重要である。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (45 results)

All 2021 2020 2019 2018 2017

All Journal Article (16 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 16 results,  Open Access: 8 results,  Acknowledgement Compliant: 1 results) Presentation (28 results) (of which Int'l Joint Research: 8 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Inflammasomes in Teleosts: Structures and Mechanisms That Induce Pyroptosis during Bacterial Infection.2021

    • Author(s)
      Morimoto N, Kono T, Sakai M, Hikima J
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 9 Pages: 4389-4389

    • DOI

      10.3390/ijms22094389

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization and expression analysis of tandemly-replicated asc genes in the Japanese medaka, Oryzias latipes2021

    • Author(s)
      Morimoto N, Okamura Y, Kono T, Sakai M, Hikima J
    • Journal Title

      Developmental and Comparative Immunology

      Volume: 115 Pages: 103894-103894

    • DOI

      10.1016/j.dci.2020.103894

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative Analysis of Plasmid DNAs from Two Strains of <i>Photobacterium damselae</i> subsp. <i>piscicida</i> Isolated from Japan and the United States2020

    • Author(s)
      Tsuchiya Akito、Nishihara Aki、Saeki Ayumi、Teru Yuki、Aoki Takashi、Kono Tomoya、Sakai Masahiro、Hikima Jun-ichi
    • Journal Title

      Fish Pathology

      Volume: 55 Issue: 1 Pages: 18-21

    • DOI

      10.3147/jsfp.55.18

    • NAID

      130007827169

    • ISSN
      0388-788X, 1881-7335
    • Year and Date
      2020-03-15
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fish cytokines: current research and applications2020

    • Author(s)
      Sakai Masahiro、Hikima Jun-ichi、Kono Tomoya
    • Journal Title

      Fisheries Science

      Volume: 87 Issue: 1 Pages: 1-9

    • DOI

      10.1007/s12562-020-01476-4

    • NAID

      40022461712

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cytosolic Sensors for Pathogenic Viral and Bacterial Nucleic Acids in Fish2020

    • Author(s)
      Mojzesz Miriam、Rakus Krzysztof、Chadzinska Magdalena、Nakagami Kentaro、Biswas Gouranga、Sakai Masahiro、Hikima Jun-ichi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 19 Pages: 7289-7289

    • DOI

      10.3390/ijms21197289

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ASC-deficiency impairs host defense against Aeromonas hydrophila infection in Japanese medaka, Oryzias latipes.2020

    • Author(s)
      Morimoto N, Okamura Y, Maekawa S, Wang HC, Aoki T, Kono T, Sakai M, Hikima J
    • Journal Title

      Fish and Shellfish Immunology

      Volume: 105 Pages: 427-437

    • DOI

      10.1016/j.fsi.2020.07.027

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular characterization and expression of two interleukin-17 receptor A genes on different chromosomes in Japanese medaka, Oryzias latipes2020

    • Author(s)
      Okamura Yo、Morimoto Natsuki、Sawada Shuzo、Kono Tomoya、Hikima Jun-ichi、Sakai Masahiro
    • Journal Title

      Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology

      Volume: 240 Pages: 110386-110386

    • DOI

      10.1016/j.cbpb.2019.110386

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interleukin-17A/F1 Deficiency Reduces Antimicrobial Gene Expression and Contributes to Microbiome Alterations in Intestines of Japanese medaka (Oryzias latipes)2020

    • Author(s)
      Okamura Yo、Morimoto Natsuki、Ikeda Daisuke、Mizusawa Nanami、Watabe Shugo、Miyanishi Hiroshi、Saeki Yuichi、Takeyama Haruko、Aoki Takashi、Kinoshita Masato、Kono Tomoya、Sakai Masahiro、Hikima Jun-ichi
    • Journal Title

      Frontiers in Immunology

      Volume: 11 Pages: 425-425

    • DOI

      10.3389/fimmu.2020.00425

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Interleukin-17A/F1 from Japanese pufferfish (Takifugu rubripes) stimulates the immune response in head kidney and intestinal cells2020

    • Author(s)
      Takahashi Yoshie、Okamura Yo、Morimoto Natsuki、Mihara Koshin、Maekawa Shun、Wang Han-Ching、Aoki Takashi、Kono Tomoya、Sakai Masahiro、Hikima Jun-ichi
    • Journal Title

      Fish & Shellfish Immunology

      Volume: 103 Pages: 143-149

    • DOI

      10.1016/j.fsi.2020.05.016

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular characterization and expression of the teleost cytosolic DNA sensor genes cGAS, LSm14A, DHX9, and DHX36 in Japanese medaka, Oryzias latipes2019

    • Author(s)
      Murakami Shiori、Morimoto Natsuki、Kono Tomoya、Sakai Masahiro、Hikima Jun-ichi
    • Journal Title

      Developmental & Comparative Immunology

      Volume: 99 Pages: 103402-103402

    • DOI

      10.1016/j.dci.2019.103402

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nonconservation of TLR5 activation site in Edwardsiella tarda flagellin decreases expression of interleukin-1β and NF-κB genes in Japanese flounder, Paralichthys olivaceus2019

    • Author(s)
      Morimoto Natsuki、Kondo Masakazu、Kono Tomoya、Sakai Masahiro、Hikima Jun-ichi
    • Journal Title

      Fish & Shellfish Immunology

      Volume: 87 Pages: 765-771

    • DOI

      10.1016/j.fsi.2019.02.024

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Circadian oscilattion of TNF-alpha gene expression regulated by clock gene, BMAL1 and CLOCK1 in the Japanese medaka (Oryzias latipes)2019

    • Author(s)
      Teika Onoue, Goshi Nishi, Jun-ichi Hikima, Masahiro Sakai and Tomoya Kono
    • Journal Title

      International Immunopharmacology

      Volume: 70 Pages: 362-371

    • DOI

      10.1016/j.intimp.2019.02.004

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular Characterization and Expression Analysis of Tumor Necrosis Factor Alpha-induced Protein 3 (TNFAIP3/A20) Gene from Japanese Pufferfish <i>Takifugu rubripes</i>2017

    • Author(s)
      Hikima J, Morita M, Kinoshita S, Basu M, Biswas G, Kono T, Sakai M
    • Journal Title

      Fish Pathology

      Volume: 52 Issue: 1 Pages: 15-22

    • DOI

      10.3147/jsfp.52.15

    • NAID

      130005548817

    • ISSN
      0388-788X, 1881-7335
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Complete Genome Sequence of Photobacterium damselae subsp. piscicida Strain OT-51443 Isolated from Yellowtail (Seriola quinqueradiata) in Japan.2017

    • Author(s)
      Aoki T, Teru Y, Kono T, Sakai M , Takano T, Hawke JP, Fukuda Y, Takeyama H, Hikima J.
    • Journal Title

      Genome Announcements

      Volume: 5 Issue: 21

    • DOI

      10.1128/genomea.00404-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Whole Genome Sequence of Photobacterium damselae subsp. piscicida strain 91-197 isolated from hybrid striped bass (Morone sp.) in the USA2017

    • Author(s)
      Teru Y, Hikima J, Kono T, Sakai M, Takano T, Hawke JP, Takeyama H, Aoki T
    • Journal Title

      Genome Announcements

      Volume: 5 Issue: 29

    • DOI

      10.1128/genomea.00600-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transcriptome analysis of immune response against Vibrio harveyi infection in orange-spotted grouper (Epinephelus coioides)2017

    • Author(s)
      Maekawa S, Byadgi O, Chen YC, Aoki T, Takeyama H, Yoshida T, Hikima J, Sakai M, Wang PC, Chen SC
    • Journal Title

      Fish Shellfish Immunol

      Volume: 70 Pages: 628-637

    • DOI

      10.1016/j.fsi.2017.09.052

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 魚類の病原微生物に対する自然免疫システムに関する研究2021

    • Author(s)
      引間 順一
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] メダカにおける3 つのasc遺伝子の同定および発現解析2021

    • Author(s)
      森本和月、岡村 洋、河野智哉、酒井正博、引間順一
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ゲノム編集メダカを用いた魚病最近に対するカスパーゼ1の役割2021

    • Author(s)
      押川周平、森本和月、木下政人、河野智哉、酒井正博、引間順一
    • Organizer
      令和3年度日本魚病学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ASC変異メダカにおけるAeromonas hydrophila感染時の細胞死誘導機構2020

    • Author(s)
      森本和月、岡村 洋、河野智哉、酒井正博、引間順一
    • Organizer
      令和2年度日本魚病学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ASC変異メダカにおけるAeromonas hydrophila 感染時の細胞死誘導機構2020

    • Author(s)
      森本和月、岡村 洋、河野智哉、酒井正博、引間順一
    • Organizer
      令和2年度日本魚病学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ASC-mutated Japanese medaka Oryzias latipes byCRISPR-Cas9 system obtains resistance to infection of Edwardsiella piscicida2019

    • Author(s)
      J. Hikima, N. Morimoto, Y. Okamura, T. Kono, M. Sakai
    • Organizer
      7th Annual Meeting of the International Cytokine & Interferon Society
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Immune responses to bacterial infection in an ASC-mutated Japanese medaka (Oryzias latipes) established by CRISPR-Cas9 system2019

    • Author(s)
      N. Morimoto, Y. Okamura, S. Maekawa, H.C. Wang, T. Aoki, T. Kono, M. Sakai, J. Hikima
    • Organizer
      Marine Biotechnology Conference 2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] Interaction between the soluble- and membrane-forms of TLR5 induces expression of IL-1β gene in Japanese flounder, Paralichthys olivaceus2019

    • Author(s)
      M. Sakai, N. Morimoto, T. Kono, J. Hikima
    • Organizer
      International Conference on Diseases of Fish and Shellfish (19th EAFP Porto 2019)
    • Related Report
      2019 Annual Research Report
  • [Presentation] The Soluble-Form Of Toll-Like Receptor 5 Interacts With The Membrane-Form and Induces Expression Of Interleukin-1β Gene In Japanese Flounder, Paralichthys olivaceus2019

    • Author(s)
      Jun-ichi Hikima, Natsuki Morimoto, Masakazu Kondo, Tomoya Kono, Masahiro Sakai
    • Organizer
      World Brackishwater Aquaculture Conference BRAQCON2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ASC 変異メダカにおける細菌感染時の炎症性サイトカイン遺伝子の発現動態2019

    • Author(s)
      森本和月、河野智哉、酒井正博、引間順一
    • Organizer
      平成30年日本魚病学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] インターロイキン17 受容体 A 遺伝子変異メダカの作製2019

    • Author(s)
      岡村 洋、河野智哉、酒井正博、引間順一、木下政人
    • Organizer
      平成30年日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 炎症応答関連分子ASC遺伝子変異メダカの作出2018

    • Author(s)
      森本和月、中崎 司、竹山春子、河野智哉、酒井正博、引間順一、青木 宙
    • Organizer
      平成30年度マリンバイオテクノロジー学会宮崎大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ヒラメ分泌型TLR5および膜型TLR5の相互作用による免疫関連遺伝子の発現誘導機構2018

    • Author(s)
      森本和月、近藤昌和、河野智哉、酒井正博、引間順一
    • Organizer
      平成30年度マリンバイオテクノロジー学会宮崎大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メダカcGASおよびLSm14A遺伝子の発現動態の解明2018

    • Author(s)
      村上栞理、森本和月、河野智哉、酒井正博、引間順一
    • Organizer
      平成30年度マリンバイオテクノロジー学会宮崎大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Characterization and expression analysis of cytosolic DNA sensor genes in Japanese medaka, Oryzias latipes2018

    • Author(s)
      Shiori Murakami, Natsuki Morimoto, Gouranga Biswas, Tomoya Kono, Masahiro Sakai, Jun-ichi Hikima
    • Organizer
      14th International Society of Developmental and Comparative Immunology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Interaction of soluble- and membrane-forms of toll-like receptor 5 induce expression of interleukin-1β gene in Japanese flounder, Paralichthys olivaceus2018

    • Author(s)
      Natsuki Morimoto, Masakazu Kondo, Tomoya Kono, Masahiro Sakai, Jun-ichi Hikima
    • Organizer
      8th International Symposium of Aquatic Animal Health
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Edwardsiella tarda感染メダカにおけるASCの役割2018

    • Author(s)
      森本和月、青木 宙、河野智哉、酒井正博、引間順一
    • Organizer
      平成30年日本魚病学会秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ヒラメ分泌型TLR5によるIL-1β遺伝子の発現誘導機構の解明2018

    • Author(s)
      森本和月、近藤昌和、河野智哉、酒井正博、引間順一
    • Organizer
      平成30年日本水産学会秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 炎症応答関連分子ASC遺伝子の特徴および変異メダカの作製2018

    • Author(s)
      引間順一、中崎 司、森本和月、前川 峻、王 涵青、青木 宙、河野智哉、酒井正博
    • Organizer
      平成30年度日本水産学会春季大会(東京海洋大学、品川、東京)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Transcriptomic analysis in the intestine of IL-17A/F1-knockout medaka, Oryzias latipes.2017

    • Author(s)
      Hikima, J, Ikeda, D, Izumi, M, Nagaoka, M, Morimoto, N, Kono, T, Sakai, M, Takeyama, H, Aoki, T
    • Organizer
      11th Asia-Pacific Marine Biotechnology Conference (Honolulu, Hawaii, USA)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] メダカ腸管におけるIL-17A/F1の役割について2017

    • Author(s)
      引間順一、池田大介、和泉幹久、森本和月、河野智哉、酒井正博、竹山春子、青木 宙、水澤奈々美、渡部終五、木下政人
    • Organizer
      マリンバイオテクノロジー学会大会(東北大学、宮城・仙台)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Photobacterium damselae subsp. piscicida 日本分離株および米国分離株のゲノム比較解析2017

    • Author(s)
      輝 祐希、引間順一、河野智哉、酒井正博、高野倫一、John P. Hawke、竹山春子、青木 宙
    • Organizer
      平成29年度日本魚病学会秋季大会(ホテルメリージュ宮崎、宮崎)
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒラメ分泌型TLR5およびフラジェリンによるインターロイキン1β遺伝子の発現誘導2017

    • Author(s)
      森本和月、河野智哉、近藤昌和、酒井正博、引間順一
    • Organizer
      平成29年度日本魚病学会秋季大会(ホテルメリージュ宮崎、宮崎)
    • Related Report
      2017 Annual Research Report
  • [Presentation] メダカcGASおよびLSm14A遺伝子の構造解析および発現解析2017

    • Author(s)
      村上栞理、森本 和月、和泉幹久、河野 智哉、酒井 正博、引間 順一
    • Organizer
      平成29年度日本魚病学会秋季大会(ホテルメリージュ宮崎、宮崎)
    • Related Report
      2017 Annual Research Report
  • [Presentation] メダカCaspase-1遺伝子の同定および遺伝子発現2017

    • Author(s)
      和泉幹久、河野智哉、酒井正博、引間順一
    • Organizer
      平成29年度日本魚病学会秋季大会(ホテルメリージュ宮崎、宮崎)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Whole genome sequences of Photobacterium damselae subsp. piscicida isolated in Japan and the USA2017

    • Author(s)
      Yuki Teru, Tomoya Kono, Masahiro Sakai, Tomokazu Takano, John P. Hawke, Yutaka Fukuda, Haruko Takeyama, Takashi Aoki, Jun-ichi Hikima
    • Organizer
      The JSFS 85th Anniversary-Commemorative International Symposium (Tokyo University of Marine Science and Technology, Shinagawa, Tokyo)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Two bacterial flagellin genes induce expression of inflammatory cytokine genes in the Japanese flounder, Paralichthys olivaceus2017

    • Author(s)
      Nastuki Morimoto, Tomoya Kono, Masakazu Kondo, Masahiro Sakai, Jun-ichi Hikima
    • Organizer
      The JSFS 85th Anniversary-Commemorative International Symposium (Tokyo University of Marine Science and Technology, Shinagawa, Tokyo)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization and expression of inflammasome-associated genes in medaka, Oryzias latipes2017

    • Author(s)
      Mikihisa Izumi, Gouranga Biswas, Tomoya Kono, Masahiro Sakai, Jun-ichi Hikima
    • Organizer
      The JSFS 85th Anniversary-Commemorative International Symposium (Tokyo University of Marine Science and Technology, Shinagawa, Tokyo)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Book] Immune system in teleosts. Edwin L. Cooper (Ed.) Advances in Comparative Immunology2018

    • Author(s)
      Teruyuki Nakanishi, Jun-ichi Hikima, Takashi Yada
    • Total Pages
      1107
    • Publisher
      Springer International Publishing
    • ISBN
      9783319767673
    • Related Report
      2018 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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