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Development of host strains for the production of gametes through germline chimera in marine fish

Research Project

Project/Area Number 17H03873
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic life science
Research InstitutionEhime University

Principal Investigator

Goto Rie  愛媛大学, 南予水産研究センター, 准教授 (70399997)

Co-Investigator(Kenkyū-buntansha) 松原 孝博  愛媛大学, 南予水産研究センター, 教授 (60443389)
斎藤 大樹  愛媛大学, 南予水産研究センター, 准教授(特定教員) (90396309)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywordsスマ / ゲノム編集 / 次世代育種システム / 不妊化 / 借腹 / 低妊化 / 生殖細胞 / 借腹生産 / 宿主開発 / 生殖系列キメラ / 細胞分取装置 / キメラ / 始原生殖細胞 / マグロ類 / 性分化 / dnd / tyr / slc24a5 / CRISPR / 生殖細胞移植 / tyrosinase / dead end / 生物工学 / 海産魚
Outline of Final Research Achievements

A new system for fish seedling production through germline chimera will be able to breed tuna species which is believed to be difficult to perform a conventional selective breeding. This system is based on fish surrogate propagation that produce donor-derived gametes through host fish by transplanting donor germ cells. In this study, we aimed to develop methods for inducing sterilized host in small tuna species, kawakawa, Euthynnus affinis. First, we confirmed that genome editing using TALEN and CRISPR/Cas9 was able to edit body color related genes in kawakawa. Then, we developed methods for inducing a complete or partial sterilization by blocking translation of dead end gene or induction of exogenous gene expression in PGC as a method without gene-modified.

Academic Significance and Societal Importance of the Research Achievements

水産庁が掲げる「みどりの食料システム戦略」では、2050年までに主要養殖魚の人工種苗比率100%など環境負荷に配慮した養殖業への転換を目指している。これら目標を達成するには、育種完全養殖は不可欠である。借腹生産が可能になると、選抜育種の困難な魚種やあらゆる形質を考慮した育種が可能になる。このことから、海産魚配偶子生産システムの必要性は今後ますます高まると考えられる。借腹
魚での効率的な配偶子生産の鍵となる宿主不妊化技術を本研究で確立できたことは、マグロ類育種にとって大きな前進である。また、育種技術の一つとしてゲノム編集に向けた基盤情報がスマで整備されたことは学術的意義が高いといえる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (15 results)

All 2020 2019 2018 2017

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results) Book (4 results)

  • [Journal Article] Sexual dichromatism and dimorphism in the goatfish Parupeneus spilurus (Perciformes: Mullidae) in southern Japan2020

    • Author(s)
      Ayumi Bandai, Takahiro Matsubara, Rie Goto, Taketo Hayakawa, Yukio Iwatsuki, Hiroyuki Motomura
    • Journal Title

      Ichthyological Research

      Volume: 67 Pages: 203-211

    • NAID

      40022132702

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Targeted mutagenesisof the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)2019

    • Author(s)
      Kentaro Higuchi, Yukinori Kazeto, Yuichi Ozaki, Toshiya Yamaguchi, Yukinori Shimada, Yoshiaki Ina, Satoshi Soma, Yoshitaka Sakakura, Rie Goto, Takahiro Matsubara, et. al
    • Journal Title

      Scientific Reports

      Volume: 9 Pages: 13871-13871

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Early development of primordial germ cells in Pacific bluefin tuna Thunnus orientalis2019

    • Author(s)
      Kentaro Higuchi, Rie Goto, Junpei Konishi, Yoshiaki Ina, Yukinori Kazeto, Koichiro Gen
    • Journal Title

      Theriogenology

      Volume: 131 Pages: 106-112

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A state-of the art review of surrogate propagation in fish2019

    • Author(s)
      Goto, R., and Saito, T.
    • Journal Title

      Theriogenology

      Volume: 133 Pages: 216-227

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of Germ-Line Chimeras in Zebrafish2019

    • Author(s)
      Saito Taiju、Goto Rie、Rivers Nicola、Yamaha Etsuro
    • Journal Title

      Methods Mol Biol.

      Volume: 1920 Pages: 327-341

    • DOI

      10.1007/978-1-4939-9009-2_20

    • ISBN
      9781493990085, 9781493990092
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 幾何学的形態計測によるスマEuthynnus affinisの形態評価の試み2019

    • Author(s)
      斎藤大樹, Milos Havelka, 西宮攻, 澤山英太郎, 後藤理恵, 松原孝博
    • Organizer
      平成31年度日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Germ line stem cell and Generation-next breeding attempt in kawakawa, Euthynnus affinis2019

    • Author(s)
      Tapas Chakraborty, Sipra Mohapatra, Eitaro Sawayama, Taiju Saito, Rie Goto, Motohiro Takagi, Yukinori Kazeto, Takahiro Matsubara
    • Organizer
      平成31年度日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] TALEN-mediated gene editing of slc24a5 (solute carrier family 24, member 5) in kawakawa, Euthynnus affinis2019

    • Author(s)
      Dipak Pandey, Rie Goto, Taiju Saito, Yukinori Kazeto, Koichiro Gen, Tetsushi Sakuma, Takashi Yamamoto, Takahiro Matsubara
    • Organizer
      平成31年度日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Induction of sterilized host by knockdown of dead end gene in kawakawa, Euthynnus affinis for the surrogate propagation2018

    • Author(s)
      Rie Goto, Taketo Hayakawa, Dipak Pandey, Kentaro Nakajima, Hirofumi Yamashita, Taiju Saito, Takahiro Matsubara
    • Organizer
      Eighth International Symposium On Vertebrate Sex Determination
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genome-editing using TALENs in kawakawa, Euthynnus affinis.2018

    • Author(s)
      Rie Goto, Dipak Pandey, Ryoko Kawata, Taketo Hayakawa, Yukinori Kazeto, Koichiro Gen, Tetsushi Sakuma, Takashi Yamamoto, Taiju Saito, Takahiro Matsubara
    • Organizer
      3rd aquaculture conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] スマ Euthynnus affinis における不妊化個体の作出2017

    • Author(s)
      早川嵩人・後藤理恵・斎藤大樹・Dipak Pandey・松原孝博
    • Organizer
      日本水産増殖学会第16回大会
    • Related Report
      2017 Annual Research Report
  • [Book] Development of Marine Fish: Several Procedures for the Observation of Embryonic Development, Kazuo Inaba and Jason M. Hall-Spencer ed., Japanese Marine Life, 125-148.2020

    • Author(s)
      Yamaha, E., Goto, R., Saito, T., Takahashi, E., Matsubara, T., Arai, K
    • Total Pages
      24
    • Publisher
      Springer
    • Related Report
      2020 Annual Research Report
  • [Book] Production of Germ-Line Chimeras in Zebrafish. In: Pelegri F. (eds) Vertebrate Embryogenesis2019

    • Author(s)
      Saito T., Goto R., Rivers N., Yamaha E.
    • Total Pages
      15
    • Publisher
      Humana Press, New York, NY
    • ISBN
      9781493990092
    • Related Report
      2018 Annual Research Report
  • [Book] Microinjection of Marine Fish Eggs. In: Liu C., Du Y. (eds) Microinjection. Methods in Molecular Biology2018

    • Author(s)
      Goto R., Saito T., Matsubara T., Yamaha E
    • Total Pages
      13
    • Publisher
      Humana Press, New York, NY
    • ISBN
      9781493988310
    • Related Report
      2018 Annual Research Report
  • [Book] ラボ必携 フローサイトメトリーQ&A2017

    • Author(s)
      戸村道夫、斎藤大樹、後藤理恵、他57名
    • Total Pages
      312
    • Publisher
      羊土社
    • ISBN
      9784758122351
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2024-12-25  

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