| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
In this study, we aimed to identify "authentic totipotent cells" that are presumably existed in MuERV-L-positive cells. We found a culture condition that efficiently induced MuERV-L-positive cells. Ascorbic acid promoted the induction of MuERV-L-positive cells while insulin inhibited it. Gene expression analysis and experiments using inhibitors revealed that the conversion of the energy metabolic pathway from glycolytic to oxidative phosphorylation is critical for the transition from MuERV-L negative cells to MuERV-L-positive cells. Furthermore, we found a subpopulation of MuERV-L-positive cells with a lipid droplet, a structure specific to the totipotent oocytes.
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