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Establishment of new biological nitrogen fixation technology by plant-endophyte-bacterial symbiotic system

Research Project

Project/Area Number 17H03948
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionIbaraki University

Principal Investigator

Narisawa Kazuhiko  茨城大学, 農学部, 教授 (90431650)

Co-Investigator(Kenkyū-buntansha) 西澤 智康  茨城大学, 農学部, 准教授 (40722111)
太田 寛行  茨城大学, 農学部, 理事 (80168947)
下田 宜司  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 主任研究員 (80415455)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Keywords根部エンドファイト / 内外生バクテリア / 共生 / エンドファイト / 内生バクテリア / 窒素固定
Outline of Final Research Achievements

In recent years, it has been shown that bacteria play an important role in forming a symbiotic relationship between fungi and plants. It has been suggested that multiple types of bacteria interact with the root endophyte V. simplex Y34, but the details are unknown. Therefore, we first tested R. pusense Y9, which was isolated from V. simplex Y34, and found that the bacterium changed the colony morphology of V. simplex Y34 and improved the conidial formation rate. Next, we investigated the bacterial community on the hyphae of V. simplex Y34 in the field, and revealed that differences and diversity of bacterial community significantly affect plant growth through V. simplex Y34.

Academic Significance and Societal Importance of the Research Achievements

本研究により、V. simplex Y34は異なる土壌条件下において土壌由来の細菌種から細菌を選抜し、菌糸圏細菌叢を構築していることが示唆された 。この新しい知見は、 自然界における植物 -菌類生態の理解やその利用において、環境ごとに細菌 -菌類間の相互作用を明らかにすることの必要性を示している。さらに、単独生物に注目するのではなく、植物-エンドファイト-細菌を1つの系としてとらえ、その相互作用や生態を学び、さらに植物根内の環境微生物叢制御技術を確立することで、これまでに実用化が困難であった共生微生物の利用技術に新たな方向性も示している。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (12 results)

All 2019 2018 2017

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results) Presentation (6 results) (of which Invited: 1 results)

  • [Journal Article] Core microbiomes for sustainable agroecosystems.2018

    • Author(s)
      Hirokazu Toju, Kabir G. Peay, Masato Yamamichi, Kazuhiko Narisawa, Kei Hiruma, Ken Naito, Shinji Fukuda, Masayuki Ushio, Shinji Nakaoka, Yusuke Onoda, Kentaro Yoshida, Klaus Schlaeppi, Yang Bai, Ryo Sugiura, Yasunori Ichihashi, Kiwamu Minamisawa and E. Toby Kiers.
    • Journal Title

      Nature Plants

      Volume: 4 Pages: 247-257

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Complete genome sequence of Agrobacterium pusense VsBac-Y9, a bacterial symbiont of the dark septate endophytic fungus Veronaeopsis simplex Y34 with potential for improving fungal colonization in roots.2018

    • Author(s)
      Y. Guo, Y. Matsuoka, T. Miura, T. Nishizawa, H. Ohta, K. Narisawa.
    • Journal Title

      Journal of Biotechnology

      Volume: 284 Pages: 31-36

    • DOI

      10.1016/j.jbiotec.2018.07.045

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fungus-Bacterium Symbionts Promote Plant Health and Performance2018

    • Author(s)
      Y. Guo, K. Narisawa
    • Journal Title

      Microbes and Environments

      Volume: 33 Issue: 3 Pages: 239-241

    • DOI

      10.1264/jsme2.ME3303rh

    • NAID

      130007494404

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2018 Annual Research Report
  • [Journal Article] Effects of <i>Rhizobium</i> Species Living with the Dark Septate Endophytic Fungus <i>Veronaeopsis simplex</i> on Organic Substrate Utilization by the Host2018

    • Author(s)
      Guo,Y., Y.Matsuoka, T.Nishizawa, H.Ohta, K.Narisawa
    • Journal Title

      Microbes and Environments

      Volume: 33 Issue: 1 Pages: 102-106

    • DOI

      10.1264/jsme2.ME17144

    • NAID

      130006581751

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The inhibitory role of dark septate endophytic fungus Phialocephala fortinii against Fusarium disease on the Asparagus officinalis growth in organic source conditions.2018

    • Author(s)
      Surono, Narisawa K.
    • Journal Title

      Biological Control

      Volume: 121 Pages: 159-167

    • DOI

      10.1016/j.biocontrol.2018.02.017

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The dark septate endophytic fungus Phialocephala fortinii is a potential decomposer of soil organic compounds and a promoter of Asparagus officinalis growth.2017

    • Author(s)
      Surono, Narisawa K.
    • Journal Title

      Fungal Ecology

      Volume: 28 Pages: 1-10

    • DOI

      10.1016/j.funeco.2017.04.001

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] 根部エンドファイトVeronaeopsis simplex Y34の内外生細菌Rhizobium pusense Y92019

    • Author(s)
      橋本実佳、郭永、西澤智康、成澤才彦
    • Organizer
      日本土壌微生物学会2019年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ストレス条件下において根部エンドファイトVeronaeopsis simplex Y34 がエンドウ生育に及ぼす影響2019

    • Author(s)
      山崎亜耶乃、成澤才彦
    • Organizer
      日本土壌微生物学会2019年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Veronaeopsis simplex Y34資材を用いたハウス栽培におけるトマト根部微生物叢への影響2018

    • Author(s)
      野口愛、高田圭太、坂上伸生、郭永、西澤智康、太田寛行、成澤才彦
    • Organizer
      日本土壌微生物学会2018年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 根部エンドファイトVeronaeopsis simplex Y34 から分離された Rhizobium pusense Y9 株とその近縁種が宿主に及ぼす影響2018

    • Author(s)
      橋本実佳、郭永、西澤智康、成澤才彦
    • Organizer
      日本土壌微生物学会2018年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] マメ科植物における根部エンドファイトの接種効果の検証2018

    • Author(s)
      下田 宜司、成澤 才彦
    • Organizer
      植物微生物研究会 第28回研究交流会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 共に生きる菌類と細菌―内生細菌は宿主菌類をどう変えるのか?2017

    • Author(s)
      高島勇介、成澤才彦
    • Organizer
      環境微生物系学会合同大会2017 企画シンポジウムS06:共生微生物―我々の理解はどこまで進んだのか?
    • Related Report
      2017 Annual Research Report
    • Invited

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Published: 2017-04-28   Modified: 2021-02-19  

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