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Exploring unknown mechanism of anaerobic soil disinfestations for encouraging reductive transitions and specific disinfestations

Research Project

Project/Area Number 17H03955
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Kobara Yuso  国立研究開発法人農業・食品産業技術総合研究機構, 農業環境変動研究センター, ユニット長 (20354045)

Co-Investigator(Kenkyū-buntansha) 宇佐見 俊行  千葉大学, 大学院園芸学研究科, 准教授 (50334173)
宍戸 雅宏  千葉大学, 大学院園芸学研究科, 教授 (80302537)
天知 誠吾  千葉大学, 大学院園芸学研究科, 教授 (80323393)
門馬 法明  公益財団法人園芸植物育種研究所, その他部局等, 科長 (80469626)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords土壌還元消毒 / 低濃度エタノール / フザリウム / センチュウ / トマト萎凋病菌 / ウリ科植物黒点根腐病菌 / 半身萎凋病菌 / 検定法 / 黒根病菌 / 白紋羽病菌 / 環境調和型農業 / 還元化促進土場 / 選択的消毒メカニズム / 還元化促進土壌 / 環境調和型農林水産
Outline of Final Research Achievements

Detailed mechanisms of anaerobic soil disinfestation with diluted ethanol (Et-ASD) were investigated on physicochemical and biological factors such as organic matter content, water-soluble organic matter fraction, soil microorganisms, etc. by comparing some reduction-promoting soils with non-reduction-promoting soils under several temperature conditions.
Especially, for Et-ASD treatment under low temperature conditions, the factors were confirmed whether these exerted density-reducing effects of soil borne pathogens in vitro between two soil types. It was proved that identified factors were applied to non-reduction-promoting soils to enhance ASD efficiency even under the low temperature condition.

Academic Significance and Societal Importance of the Research Achievements

土壌還元消毒効果は、特に低温下で効果が不安定になる欠点があった。この要因が明らかになり、この物理化学的、生物的要因を土壌還元消毒効果が不安定な土壌に付与する事によって、土壌還元消毒効果が改善した。すなわち、幅広い作物の土壌伝染性病原微生物に対して、冷涼な地域や低温期でも安定した土壌還元消毒効果が発揮できることで、低濃度エタノールを用いた土壌還元消毒は世界中で利用可能な革新的な汎用技術となり得ることが実証できた。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (17 results)

All 2020 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (16 results) (of which Int'l Joint Research: 7 results)

  • [Journal Article] PCR primers for identification and detection of Berkeleyomyces rouxiae, a causal agent of black root rot of lettuce2020

    • Author(s)
      Nakane, R., Usami, T.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 86

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 低濃度エタノールを用いた土壌還元消毒法によるトマト栽培でのネコブセンチュウ・フザリウム対策の現地実証試験について2020

    • Author(s)
      小原 裕三、北岡大知、作田光洋、門馬法明
    • Organizer
      日本農薬学会第45回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Berkeleyomyces rouxiaeに特異的なプライマーを用いたレタス黒根病の診断2020

    • Author(s)
      中根 麗・宇佐見俊行
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ナシ白紋羽病菌に対する土壌還元消毒法の適用性2020

    • Author(s)
      櫻井えり花・門馬法明・高橋真秀・小原 均・村田義宏・宍戸雅宏
    • Organizer
      日本土壌微生物学会2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 還元消毒処理土壌中におけるトマト萎凋病菌密度の土壌の化学性の経時変化2020

    • Author(s)
      門馬法明・白根正太・天知誠吾
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] OPTIMIZED SOIL DISINFESTATION TRIALS AGAINST FUNGAL PATHOGENS OF TOMATOES2019

    • Author(s)
      Yuso Kobara, Noriaki, Momma, Mitsuo Horita
    • Organizer
      2019 MBAO: Fumigation and Alternatives for Production, Storage and Trade Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] レタス黒根病菌に対する土壌還元消毒の効果2019

    • Author(s)
      中根 麗・宇佐見俊行
    • Organizer
      日本土壌微生物学会2019年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 土壌還元消毒においてVerticillium dahliaeの微小菌核を死滅させる成分2019

    • Author(s)
      柳岡 麻由子, 門馬 法明, 宇佐見 俊行
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] SOIL DISINFESTATION TRIALS AGAINST FUNGAL PATHOGENS OF LISIANTHUS PLANTS2018

    • Author(s)
      Yuso Kobara, Noriaki Momma, Ikuro Furuzono, Akira Ogata, Norihiro Yamauchi
    • Organizer
      2018 MBAO: Fumigation and Alternatives for Production, Storage and Trade Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Applicable Expansion of Anaerobic Soil Disinfestation Technique Using Diluted Ethanol in Japan2018

    • Author(s)
      Yuso Kobara, Noriaki Momma, Mitsuo Horita, Isamu Matsumoto, Yasushi Narimatsu, Daisuke Miyahara
    • Organizer
      9th International Symposium on Soil and Substrate Disinfestation (SD 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hints for optimization of anaerobic soil disinfestation2018

    • Author(s)
      Noriaki Momma, Yuso Kobara, Taro Isoyama, Seigo Amachi
    • Organizer
      9th International Symposium on Soil and Substrate Disinfestation (SD 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 低濃度エタノール消毒土壌から単離した細菌は低温でもトマト萎凋病菌を抑制する2018

    • Author(s)
      磯山太郎、堀知行、門馬法明、宇佐美俊行、天知誠吾
    • Organizer
      日本微生物生態学会第32回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Possible mechanisms of anaerobic soil disinfestation (ASD) using low concentration of ethanol as a carbon source2018

    • Author(s)
      Taro Isoyama, Noriaki Momma, Toshiyuki Usami, Tmomoyuki Hori, Seigo Amachi
    • Organizer
      9th International Symposium on Soil and Substrate Disinfestation (SD 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 炭素源の相違が土壌表層部と下層部における還元消毒効果に及ぼす影響2018

    • Author(s)
      嶋本和也,門馬法明,天知誠吾,小原裕三,宇佐見俊行,宍戸雅宏
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] NEW APPROACHES BY ANAEROBIC SOIL DISINFESTATION FOR STRAWBERRY CULTIVATION MEDIA2017

    • Author(s)
      Yuso Kobara, Noriaki Momma, Ysushi Narimatsu, Isamu Matsumoto, Daisuke Miyahara
    • Organizer
      2017 Annual International Research Conference on Methyl Bromide Alternatives and Emissions Reductions
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CURRENT STATUS OF DISSEMINATION OF ASD IN JAPAN AND FUTURE DIRECTIONS2017

    • Author(s)
      Noriaki Momma, Kazuya Shimamoto, Yuso Kobara
    • Organizer
      2017 Annual International Research Conference on Methyl Bromide Alternatives and Emissions Reductions
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 低濃度エタノールを用いた土壌還元消毒法における殺菌メカニズムの解明2017

    • Author(s)
      磯山太郎, 堀知行, 門馬法明, 宇佐見俊行, 天知誠吾
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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