| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2021: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2020: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Outline of Final Research Achievements |
We found the CDP-glycerol (CDP-Gro), which is a novel sugar alcohol nucleotide donor in mammals, and identified that the PCYT2 is CDP-Gro synthase. The biosynthesis of functional O-mannosyl glycans require the formation of tandem ribitol phosphate structure by transfer from CDP-ribitol (CDP-Rbo), but glycerol phosphate (GroP) transferred from CDP-Gro inhibited the formation of tandem ribitol phosphate structure. We also determined the crystal structures of POMGNT2, POMK, and FKRP, which are biosynthetic enzymes of O-mannosyl glycans in mammals, revealing the detailed mechanism of the glycosylation by each enzyme.
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