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Studies on a novel regulatory mechanisms for neurotransmission through controlling the morphology of spine neck

Research Project

Project/Area Number 17H04014
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionOsaka University

Principal Investigator

SATO MAKOTO  大阪大学, 連合小児発達学研究科, 教授 (10222019)

Co-Investigator(Kenkyū-buntansha) 岡 雄一郎  大阪大学, 連合小児発達学研究科, 講師 (30614432)
TIONG Sheena  大阪大学, 連合小児発達学研究科, 特任講師(常勤) (80771815)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywords細胞機能形態学 / 解剖学 / 神経科学 / 脳・神経 / 細胞・組織
Outline of Final Research Achievements

Learning induces morphological changes of the postsynaptic spines. However, 3D structure of the spine has not been intensively investigated. We here study the fine 3D structure of spines of hippocampal neurons by reconstructing the whole spine images using FIB-SEM. FIB-SEM allows us to examine the whole structure of the spine at high resolution. Unexpectedly, most stubby type spines, of which neck widths appeared to be as wide as the head diameters, belonged to the mushroom type; their neck widths were much narrow when they were observed from the different angles. This indicates that most necks of the spines are narrower compared to the heads, suggesting that the spine neck works as the limiting region for the receptor transports between the dendritic shafts and the spine heads where synaptic transmission occurs.

Academic Significance and Societal Importance of the Research Achievements

後シナプス側であるスパインの形態変化は、学習の細胞基盤の一つである。しかし必ずしもその形態が正確に評価できていなかった。そこで、FIB-SEMを用いて、スパインの形態の正確な評価を試みた。結果、従来スパインの頚部が太くstubby型と考えられていたスパインのほとんどが、別角度からの観察では頚部の細いマッシュルーム型であった。また、頚部は従来考えられていたより細く、樹状突起とスパイン頭部との間を行き来する受容体にとり、頚部が律速部位となりうること、つまり、シナプス可塑性の一つの制御部位である可能性が考えられた。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (47 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (6 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (31 results) (of which Int'l Joint Research: 4 results,  Invited: 5 results) Remarks (3 results)

  • [Int'l Joint Research] マラヤ大学(マレーシア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] 紹興市人民医院(中国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] カールスルーエ工科大学(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] マラヤ大学(マレーシア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 紹興市人民医院(中国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] カールスルーエ工科大学(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Pathogenic POGZ mutation causes impaired cortical development and reversible autism-like phenotypes.2020

    • Author(s)
      Matsumura K, (33名中略), Hashimoto R, (37名中35番目),Hashimoto H, Nakazawa T.
    • Journal Title

      Nat. Commun

      Volume: 11(1) Issue: 1 Pages: 859-859

    • DOI

      10.1038/s41467-020-14697-z

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PIP3-Phldb2 is crucial for LTP regulating synaptic NMDA and AMPA receptor density and PSD95 turnover2019

    • Author(s)
      Xie Min-Jue、Ishikawa Yasuyuki、Yagi Hideshi、Iguchi Tokuichi、Oka Yuichiro、Kuroda Kazuki、Iwata Keiko、Kiyonari Hiroshi、Matsuda Shinji、Matsuzaki Hideo、Yuzaki Michisuke、Fukazawa Yugo、Sato Makoto
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 4305-4305

    • DOI

      10.1038/s41598-019-40838-6

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autism-associated protein kinase D2 regulates embryonic cortical neuron development.2019

    • Author(s)
      Matsumura K, Baba M, Nagayasu K, Yamamoto K, Kondo M, Kitagawa K, Takemoto T, Seiriki K, Kasai A, Ago Y, Hayata-Takano A, Shintani N, Kuriu T, Iguchi T, Sato M, Takuma K, Hashimoto R, Hashimoto H.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 519(3) Issue: 3 Pages: 626-632

    • DOI

      10.1016/j.bbrc.2019.09.048

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Kcnab1 is expressed in subplate neurons with unilateral long-range inter-areal projections2019

    • Author(s)
      Tiong Y.X.S., Oka Y., Sasaki T., Taniguchi M., Doi M., Akiyama H., Sato, M.
    • Journal Title

      Frontiers in Neuroanatomy

      Volume: 印刷中 Pages: 39-39

    • DOI

      10.3389/fnana.2019.00039

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Subcellular distribution of non-muscle myosin IIb is controlled by FILIP through Hsc70.2017

    • Author(s)
      Yagi H, Takabayashi T, Xie MJ, Kuroda K, Sato M
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 2 Pages: e0172257-e0172257

    • DOI

      10.1371/journal.pone.0172257

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effects of Buyang Huanwu decoction combined with edaravone on mitochondrial apoptotic pathway of nerve cells in mice with cerebral ischemia-reperfusion injury2017

    • Author(s)
      Zhong, F.-F., Wu, C.-L., Zhang, Y.-X., Sun, X.-F., Xie, M.-J., Sato, M
    • Journal Title

      Int. J. Clin. Exp. Med

      Volume: 10(4) Pages: 6749-6755

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Developmental downregulation of LIS1 expression limits axonal extension and allows axon pruning2017

    • Author(s)
      Kumamoto Kanako、Iguchi Tokuichi、Ishida Ryuichi、Uemura Takuya、Sato Makoto、Hirotsune Shinji
    • Journal Title

      Biology Open

      Volume: 6 Pages: 1041-1055

    • DOI

      10.1242/bio.025999

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Neocortical neurons develop neuronal circuits for cooperative tasks by multiple-targeting with collateral formation.2020

    • Author(s)
      Makoto Sato
    • Organizer
      第2回 OIST-OU Joint Symposium
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] An early subplate marker Kcnab1 is expressed in cortico-cortical projection neurons in layer 6b in the mouse neocortex.2020

    • Author(s)
      Oka Yuichiro, Tiong Sheena Y.X., Yahaya Murtala Hamza, Sasaki, Tatsuya, Doi Miyuki, Yasumura Misato, Taniguchi Manabu, Sato Makoto.
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 発達段階の大脳新皮質において、Hsd11b1発現領域は一時的に拡大する2020

    • Author(s)
      土井美幸、岡雄一郎、佐藤真
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Kcnab1 is an early marker for subplate of the mouse cerebral cortex and expressed in cortico-cortical projection neurons in layer 6b2019

    • Author(s)
      Yuichiro Oka, Sheena Y. X. Tiong, Tatsuya Sasaki, Miyuki Doi, Manabu Taniguchi, Makoto Sato
    • Organizer
      第42回日本神経学会大会/第62回日本神経化学大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] LAR-RPTPs dimer formation: a potential key-step in the receptors activation control2019

    • Author(s)
      Mai Q Nguyen, Tokuichi Iguchi, Misato Yasumura, Makoto Sato
    • Organizer
      第42回日本神経学会大会/第62回日本神経化学大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 単一大脳皮質2/3層ニューロンによる長連合性および交連性回路の形成過程の解析2019

    • Author(s)
      岡 雄一郎、土井 美幸、谷口 学、猪口 徳一、佐藤 真
    • Organizer
      第46回日本脳科学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] FIB-SEMを用いたPSD陽性樹状突起スパイン形態の解析2019

    • Author(s)
      三田村耕平、猪口徳一、安村美里、岡雄一郎、黒田一樹、謝敏カク、Ruwaida Elhanbaly、深澤有吾、佐藤真
    • Organizer
      第95回日本解剖学会近畿支部学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 受容体型チロシン脱リン酸化酵素を介した皮質脊髄路軸索側枝形成の分子機構の解析2019

    • Author(s)
      安村美里、猪口徳一、Nguyen Quynh Mai、三田村耕平、吉田知之、佐藤真
    • Organizer
      第92回日本薬理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 脳内局所環境感知を担う受容体による軸索側枝制御機構2019

    • Author(s)
      猪口 徳一、安村 美里、Nguyen Quynh Mai、三田村 耕平、吉田 知之、岡 雄一郎、佐藤 真
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Long associational projections of Plxnd1-expressing cortical neurons are formed by interstitial collaterals from the callosally-projecting primary axonal shaft2018

    • Author(s)
      Yuichiro Oka, Yuka Lin, Sheena Y.X. Tiong, Tatsuya Sasaki, Miyuki Doi, Tokuichi Iguchi, Makoto Sato
    • Organizer
      22nd Biennial Meeting of the International Society for Developmental Neuroscience
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elevated extension speed for a selected long associational collateral of a single cortical dual-projection neuron2018

    • Author(s)
      Yuichiro Oka, Yuka Lin, Sheena Y.X. Tiong, Tatsuya Sasaki, Miyuki Doi, Tokuichi Iguchi, Makoto Sato
    • Organizer
      2018 Joint Meeting between The Neurodevelopmental Biology Section of The Korean Society for Molecular and Cellular Biology and Japanese Developmental Neuroscientist
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Potassium channel subunit Kcnab1 is expressed in cortical layer 6b long-range corticocortical projection neurons2018

    • Author(s)
      Sheena Tiong, Yuichiro Oka, Tatsuya Sasaki, Makoto Sato
    • Organizer
      11th FENS Forum of Neuroscience
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Faster extension for a predetermined associational collateral of a cortical dual-projection neuron2018

    • Author(s)
      Makoto Sato
    • Organizer
      THE 5TH INTERNATIONAL ANATOMICAL SCIENCES AND CELL BIOLOGY CONFERENCE Malaysia
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 単一皮質ニューロンは軸索毎に異なる伸長速度により複数領野へ同調的に投射する2018

    • Author(s)
      岡雄一郎, 林由佳, Sheena Y. X. Tiong, 佐々木達也, 土井美幸, 猪口徳一, 佐藤真
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] バレル皮質におけるPlxnd1発現第5a層ニューロンの軸索伸展様式の解析2018

    • Author(s)
      林由佳, 岡雄一郎, 佐藤真
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 受容体型チロシンフォスファターゼを介した軸索側枝形成の分子機構の解析2018

    • Author(s)
      安村美里, 猪口徳一, Nguyen Quynh Mai, 三田村耕平, 吉田知之, 佐藤真
    • Organizer
      第41回日本神経科学大会(神戸コンベンションセンター
    • Related Report
      2018 Annual Research Report
  • [Presentation] Regulation of intra-spine distribution of myosin is associated with morphology of spine2018

    • Author(s)
      Hideshi Yagi(八木 秀司)、佐藤 真
    • Organizer
      第61回日本神経化学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Fork cell-like neurons in the mouse insular cortex2018

    • Author(s)
      服部美咲, 谷口学, 岡雄一郎, 佐藤真
    • Organizer
      第61回日本神経化学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Analysis on axonal development of Plxnd1-expressing L5a neurons in the barrel cortex2018

    • Author(s)
      Y. Lin, Y. Oka, M. Sato
    • Organizer
      第11回神経発生討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Functional analysis of receptor involvement in controlling axonal collateralization during brain development2018

    • Author(s)
      Nguyen Quynh Mai, Tokuichi Iguchi, Manabu Taniguchi, Misato Yasumura, Makoto Sato
    • Organizer
      第11回神経発生討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Inter-areal collateral branches of layer 2/3 neurons grow more rapidly than intra-areal collateral branches in the mouse neocortex2018

    • Author(s)
      Makoto Sato, Yuichiro Oka
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 脳発生における軸索側枝形成を制御する受容体群の役割について2018

    • Author(s)
      猪口 徳一、Nguyen Quynh Mai、安村 美里、吉田 知之、岡 雄一郎、佐藤 真
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 大脳皮質ニューロン軸索側枝による領野間回路形成過程の解析2018

    • Author(s)
      岡 雄一郎、林 由佳、Tiong Sheena、土井 美幸、佐々木 達也、猪口 徳一、佐藤 真
    • Organizer
      第95回日本生理学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Analysis of The Molecular Mechanism for Corticospinal Tract Collateralization2017

    • Author(s)
      Nguyen Quynh Mai, 猪口 徳一, 佐藤 真
    • Organizer
      第64回日本生化学会近畿支部例会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Molecular characterisation and axon projection pattern of deep cortical layer 6 neurons in mouse2017

    • Author(s)
      張 永欣, 岡 雄一郎, 佐藤 真
    • Organizer
      第64回日本生化学会近畿支部例会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Developmental analysis of the inter-areal connections from cortical neurons in different layers2017

    • Author(s)
      Y. Oka, Y. Lin, M. Hattori, S.Y.X. Tiong, M. Doi, T. Iguchi, M. Sato
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Antero-posterior organization of pontine nuclei contributes to the connection of cortico-ponto-cerebellar pathway through the expression of axon guidance molecules2017

    • Author(s)
      T. Iguchi, M. Omi, Y. Oka, M. Sato
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] A potassium channel subunit as a novel molecular marker for long-range projection neurons in the earliest-formed cortical layer2017

    • Author(s)
      S.Y.X. Tiong, Y. Oka, T. Sasaki, M. Sato
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Inter-laminar comparison of inter-areal circuit development in the mouse cerebral cortex2017

    • Author(s)
      M. Sato
    • Organizer
      第60回日本神経化学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Distant area-targeting axonal branches of layer 2/3 neurons develop more rapidly than locally targeting branches in mouse neocortex2017

    • Author(s)
      Y. Oka, Y. Lin, M. Hattori, S.Y.X. Tiong, M. Doi, T. Sasaki, T. Iguchi, M. Sato
    • Organizer
      第48回生理研国際シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 発生過程における一次体性感覚野内の第5層神経細胞の軸索側枝伸展2017

    • Author(s)
      林由佳, 岡雄一郎, 佐藤真
    • Organizer
      第93回日本解剖学会近畿支部学術集会
    • Related Report
      2017 Annual Research Report
  • [Remarks] 大阪大学大学院医学系研究科 解剖学講座(神経機能形態学)

    • URL

      http://www.anat2.med.osaka-u.ac.jp/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 大阪大学大学院医学系研究科解剖学講座(神経機能形態学)

    • URL

      http://www.anat2.med.osaka-u.ac.jp/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 解剖学講座(神経機能形態学)

    • URL

      http://www.anat2.med.osaka-u.ac.jp/index.html

    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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