Studies on a novel regulatory mechanisms for neurotransmission through controlling the morphology of spine neck
| Project/Area Number |
17H04014
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Osaka University |
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Principal Investigator |
SATO MAKOTO 大阪大学, 連合小児発達学研究科, 教授 (10222019)
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| Co-Investigator(Kenkyū-buntansha) |
岡 雄一郎 大阪大学, 連合小児発達学研究科, 講師 (30614432)
TIONG Sheena 大阪大学, 連合小児発達学研究科, 特任講師(常勤) (80771815)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Keywords | 細胞機能形態学 / 解剖学 / 神経科学 / 脳・神経 / 細胞・組織 |
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| Outline of Final Research Achievements |
Learning induces morphological changes of the postsynaptic spines. However, 3D structure of the spine has not been intensively investigated. We here study the fine 3D structure of spines of hippocampal neurons by reconstructing the whole spine images using FIB-SEM. FIB-SEM allows us to examine the whole structure of the spine at high resolution. Unexpectedly, most stubby type spines, of which neck widths appeared to be as wide as the head diameters, belonged to the mushroom type; their neck widths were much narrow when they were observed from the different angles. This indicates that most necks of the spines are narrower compared to the heads, suggesting that the spine neck works as the limiting region for the receptor transports between the dendritic shafts and the spine heads where synaptic transmission occurs.
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| Academic Significance and Societal Importance of the Research Achievements |
後シナプス側であるスパインの形態変化は、学習の細胞基盤の一つである。しかし必ずしもその形態が正確に評価できていなかった。そこで、FIB-SEMを用いて、スパインの形態の正確な評価を試みた。結果、従来スパインの頚部が太くstubby型と考えられていたスパインのほとんどが、別角度からの観察では頚部の細いマッシュルーム型であった。また、頚部は従来考えられていたより細く、樹状突起とスパイン頭部との間を行き来する受容体にとり、頚部が律速部位となりうること、つまり、シナプス可塑性の一つの制御部位である可能性が考えられた。
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] PIP3 -Phldb2 is crucial for LTP regulating synaptic NMDA and AMPA receptor density and PSD95 turnover.2019
Author(s)
Xie M.-J., Ishikawa Y., Yagi H., Iguchi T., Oka Y., Kuroda K., Iwata K., Kiyonari H., Matsuda S., Matsuzaki H., Yuzaki M., Fukazawa Y., Sato M.
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Journal Title
Sci Rep
Volume: 9
Pages: 4305
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Autism-associated protein kinase D2 regulates embryonic cortical neuron development.2019
Author(s)
Matsumura K., Baba M., Nagayasu K., Yamamoto K., Kondo M., Kitagawa K., Takemoto T., Seiriki K., Kasai A., Ago Y., Hayata-Takano A., Shintani N., Kuriu T., Iguchi T., Sato M., Takuma K., Hashimoto R., Hashimoto H., Nakazawa T.
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Journal Title
Biochem Bioph Res Co
Volume: 519
Pages: 626-632
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Kcnab1 is expressed in subplate neurons with unilateral long-range inter-areal projections2019
Author(s)
Tiong, YXS, Oka, Y.,Sasaki, T.,Taniguchi, M.,Doi, M., *Sato, M.
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Journal Title
Frontiers in Neuroanatomy
Volume: in press
Pages: in press
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Effects of Buyang Huanwu decoction combined with edaravone on mitochondrial apoptotic pathway of nerve cells in mice with cerebral ischemia-reperfusion injury2017
Author(s)
Zhong, F.-F., Wu, C.-L., Zhang, Y.-X., Sun, X.-F., Xie, M.-J., Sato, M
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Journal Title
Int. J. Clin. Exp. Med
Volume: 10(4)
Pages: 6749-6755
Related Report
Peer Reviewed / Int'l Joint Research
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