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Osteogenic capillaries - new aspect of endochondral ossification

Research Project

Project/Area Number 17H04015
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionKeio University

Principal Investigator

MATSUO Koichi  慶應義塾大学, 医学部(信濃町), 教授 (40229422)

Co-Investigator(Kenkyū-buntansha) 黒田 有希子  慶應義塾大学, 医学部(信濃町), 助教 (70455343)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywords耳小骨 / 聴覚骨芽細胞 / ミネラリゼーション / 蝸牛 / 骨芽細胞 / 骨形成 / 石灰化 / コラーゲン / 中耳 / 内耳 / 内軟骨性骨化 / 成長板
Outline of Final Research Achievements

Auditory ossicles in the middle ear and bony labyrinth of the inner ear are highly mineralized in adult mammals. Cellular mechanisms underlying formation of dense bone during development are unknown. Here, we found that osteoblast-like cells synthesizing highly mineralized hearing-related bones produce both type I and type II collagens as the bone matrix, while conventional osteoblasts and chondrocytes primarily produce type I and type II collagens, respectively. Furthermore, these osteoblast-like cells were not labeled in a "conventional osteoblast"-specific green fluorescent protein mouse line. Type II collagen-producing osteoblast-like cells were not chondrocytes as they express osteocalcin, localize along alizarin-labeled osteoid, and form osteocyte lacunae and canaliculi. Therefore, we conclude that these type II collagen-producing hypermineralizing osteoblasts (named auditory osteoblasts) represent a new osteoblast subtype.

Academic Significance and Societal Importance of the Research Achievements

耳小骨が硬いことにより音の伝導効率が良くなること、蝸牛骨が硬いことにより、蝸牛の構造的な安定性が増すものと考えられており、本研究はそのような硬い骨を生み出す骨芽細胞が、これまで知られていた骨芽細胞とは異なることを、いくつもの異なる研究手法を組み合わせて示した。実際に、骨の石灰化異常をきたす疾患では、難聴を認めるものも知られている。本研究は、骨代謝異常と難聴の関係を理解するための手がかりとなるものであるとともに、中耳や内耳で聴覚器を構成する骨組織に特化した骨芽細胞の存在が明らかになった。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (23 results)

All 2021 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (16 results) (of which Int'l Joint Research: 6 results,  Invited: 3 results) Remarks (1 results)

  • [Int'l Joint Research] Ludwig Boltzmann Institute of Osteology(オーストリア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Ludwig Boltzmann Institute of Osteology(オーストリア)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Hypermineralization of hearing‐related bones by a specific osteoblast subtype2021

    • Author(s)
      Yukiko Kuroda, Katsuhiro Kawaai, Naoya Hatano, Yanlin Wu, Hidekazu Takano, Atsushi Momose, Takuya Ishimoto, Takayoshi Nakano, Paul Roschger, Stephane Blouin, Koichi Matsuo
    • Journal Title

      Journal of Bone and Mineral Research

      Volume: - Issue: 8 Pages: 1535-1547

    • DOI

      10.1002/jbmr.4320

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Trans-pairing between osteoclasts and osteoblasts shapes the cranial base during development.2019

    • Author(s)
      Edamoto M, Kuroda Y, Yoda M, Kawaai K, Matsuo K.
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 297-297

    • DOI

      10.1038/s41598-018-38471-w

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bone2019

    • Author(s)
      Matsuo K, Ji S, Miya A, Yoda M, Hamada Y, Tanaka T, Takao-Kawabata R, Kawaai K, Kuroda Y, Shibata S.
    • Journal Title

      Innervation of the tibial epiphysis through the intercondylar foramen.

      Volume: 120 Pages: 297-304

    • DOI

      10.1016/j.bone.2018.11.007

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of long-term cigarette smoke exposure on bone metabolism, structure, and quality in a mouse model of emphysema2018

    • Author(s)
      Sasaki Mamoru、Chubachi Shotaro、Kameyama Naofumi、Sato Minako、Haraguchi Mizuha、Miyazaki Masaki、Takahashi Saeko、Nakano Takayoshi、Kuroda Yukiko、Betsuyaku Tomoko、Matsuo Koichi
    • Journal Title

      PLoS One

      Volume: 13 Issue: 1 Pages: e0191611-e0191611

    • DOI

      10.1371/journal.pone.0191611

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Osteoclast-Osteoblast “Trans-pairing” across Cortical Bone Shapes Developing Long Bones.2019

    • Author(s)
      Koichi Matsuo, Masaki Yoda, Yukiko Kuroda, Katsuhiko Kawaai, Yanlin Wu, Hidekazu Takano, Atsushi Momose.
    • Organizer
      米国骨代謝学会(ASBMR)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウス耳小骨のタルボ位相イメージング2019

    • Author(s)
      松尾光一
    • Organizer
      SPring-8シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 超石灰化骨芽細胞によって形成された骨はII型コラーゲンを含み骨密度が高くなる2019

    • Author(s)
      黒田有希子、河合克宏、松尾光一
    • Organizer
      第37回日本骨代謝学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 内軟骨性骨化で形成される耳小骨は軟骨原器より小さい2019

    • Author(s)
      松尾光一、黒田有希子、河合克宏、百生敦、姫しゅうてい
    • Organizer
      第37回日本骨代謝学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] X-ray Talbot imaging from bone to tunicate2019

    • Author(s)
      松尾光一
    • Organizer
      The 15th Symposium of Japanese Research Community on X-ray Imaging Optics
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 高回折効率FZPを用いたTalbot干渉計型X線位相顕微鏡.2019

    • Author(s)
      呉彦霖、高野秀和、Karol Vegso、星野真人、松尾光一、百生敦
    • Organizer
      第32回日本放射光学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Hypermineralization of bones by Col2a1-expressing osteoblasts.2018

    • Author(s)
      Yukiko Kuroda, Koichi Matsuo.
    • Organizer
      ASBMR 2018 Annual Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] X-Ray phase nano-tomography by FZP-based X-ray microscopy combined with Talbot interferometry.2018

    • Author(s)
      Yanlin Wu, Hidekazu Takano, Karol Vegso, Masato Hoshino, Koichi Matsuo, Atsushi Momose.
    • Organizer
      X-Ray Microscopy (XRM2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウス腓骨における骨形成部位は成長に伴い変移する.2018

    • Author(s)
      依田昌樹、黒田有希子、松尾光一
    • Organizer
      第36回日本骨代謝学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シュワン細胞可視化マウスにおける脛骨近位骨端の痛覚神経の解析2018

    • Author(s)
      姫しゅうてい、依田昌樹、田中智哉、高尾亮子、黒田有希子、芝田晋介、松尾光一
    • Organizer
      第36回日本骨代謝学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 骨密度の高い骨はII型コラーゲンを産生する「超石灰化骨芽細胞」によって作られる2018

    • Author(s)
      黒田有希子、依田昌樹、松尾光一
    • Organizer
      第36回日本骨代謝学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] A novel osteoblast subpopulation initiates endochondral ossification by forming osteogenic capillaries.2017

    • Author(s)
      Yukiko Kuroda, Ayako Sakamoto, Masaki Yoda, Yanlin Wu, Hidekazu Takano, Atsushi Momose, Koichi Matsuo.
    • Organizer
      American Society for Bone and Mineral Research 2017 Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The Primary Trabecular Bone Becomes Lamellar by Continuous Parathyroid Hormone Infusion2017

    • Author(s)
      Masaki Yoda, Yukiko Kuroda, Koichi Matsuo.
    • Organizer
      American Society for Bone and Mineral Research 2017 Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Developmental bone biology inspired by interferometric x-ray phase imaging2017

    • Author(s)
      Koichi Matsuo
    • Organizer
      X-ray and Neutron Phase Imaging with Gratings (XNPIG) 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Col1a1low骨芽細胞は内軟骨性骨化で働く新たな骨芽細胞群である2017

    • Author(s)
      黒田有希子、依田昌樹、松尾光一
    • Organizer
      第35回日本骨代謝学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マウス腓骨における内軟骨性骨化後の断面形状の変化2017

    • Author(s)
      依田昌樹、黒田有希子、松尾光一
    • Organizer
      第35回日本骨代謝学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Remarks] Laboratory of Cell and Tissue Biology

    • URL

      http://wp4.matsuo-lab.com/

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2022-01-27  

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