Development of in vivo recording system for analyzing disturbance of these clock gene expression rhythms in freely moving mouse

• Project/Area Number: 17H04022
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: International University of Health and Welfare
• Principal Investigator: hamada toshiyuki 国際医療福祉大学, 薬学部, 准教授 (20360208)
• Co-Investigator(Kenkyū-buntansha): 中村 孝博 明治大学, 農学部, 専任准教授 (00581985) ; サザランド ケネス・リー 北海道大学, 医学研究院, 助教 (70643914) ; 尾崎 倫孝 北海道大学, 保健科学研究院, 教授 (80256510)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
• Keywords: 生体リズム / 生体リズムの乱れ / 時計遺伝子 / freely moving / 発光計測 / ルシフェリン / 体内時計 / Period1 / 自由行動マウス / フリームービング / in vivo / 遺伝子発現計測 / 発光イメージング / in vivo イメージング / 遺伝子 / 計測工学 / 生理学 / 環境 / 脳・神経

Outline of Final Research Achievements

Clock genes express circadian rhythms in most organs. These rhythms are organized throughout the whole body, regulated by the suprachiasmatic nucleus in the brain. Disturbance of these clock gene expression rhythms is a risk factor for diseases such as obesity. We developed a multiple recording system of clock gene expression rhythm for the central nervous system and peripheral tissues in freely moving condition. Using this system, we found clock gene: Period1 response to subtle blood glucose changes with high sensitivity and elevated Period1 expression was occurred in early stage of diabetes. Present results show the elevated Period1 expression plays an important role in the aggravation of diabetes. Finally, we established a method to measure abnormal Period1 gene expression (risk factor) before the onset of severe diabetes using whole hair root tissues. These our experimental procedure is extremely simple and makes it easy to detect risk factors for severe diabetes.

Academic Significance and Societal Importance of the Research Achievements

昼夜を問わない高度情報化社会では、食生活や睡眠サイクルの乱れを誘発し生体リズムの乱れを無意識のまま過すことが多い。生体リズムの乱れは睡眠障害、糖尿病、乳癌など様々な疾患を発症する。リズムの乱れは、長期間、体の各組織の活動リズムを各々計測することで可能となる。本研究では生体各組織の時計遺伝子発現リズムを長期間リアルタイムで解析するシステムを開発し、時計遺伝子発現の一過性の上昇が糖尿病の重篤化に重要な役割をもつことを示し、糖尿病の極めて初期段階をとらえることに成功した。疾患の初期段階をとらえることは、疾患発症の予防法確立に大きく貢献できるだけでなく疾患の重篤化も防ぐことができる。

Research Products

• [Journal Article] Period1 gene expression in the olfactory bulb and liver of freely moving streptozotocin-treated diabetic mouse (2021)
  • Author(s): Kanou H, Nagasawa K, Ishi Y, Chishima A, Hayashi J, Sutherland K, Ishikawa M, Ozaki M, Shirato H., Hamada K, Hamada T
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 560
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Stability of D-luciferin for bioluminescence to detect gene expression in freely moving mice for long duration (2021)
  • Author(s): Nakajima K, Hamada K, Ito R, Yoshida Y, Sutherland K, Ishikawa M，Ozaki M, Shirato H, Hamada T
  • Journal Title: Luminescence Volume: 38 Pages: 94-98
  • DOI: 10.1002/bio.3917
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 時計遺伝子発現の自動定量化技術を用いた体内時計関連疾患発症機構解析 システムの開発 (2021)
  • Author(s): 浜田和子, 狩野晴美, 中島かな子, 浜田俊幸
  • Journal Title: Medical Science Digest・ニューサイエンス社 Volume: Vol.47.No.4
• [Journal Article] Circadian clock regulates tear secretion in the lacrimal gland, (2021)
  • Author(s): Vu CHV, Kawashima M, Nakamura W, Nakamura TJ, Tsubota K.
  • Journal Title: Experimental Eye Research Volume: 108524 Pages: 108524-108524
  • DOI: 10.1016/j.exer.2021.108524
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] GABA from vasopressin neurons regulates the time at which suprachiasmatic nucleus molecular clocks enable circadian behavior (2021)
  • Author(s): Maejima Takashi、Tsuno Yusuke、Miyazaki Shota、Tsuneoka Yousuke、Hasegawa Emi、Islam Md Tarikul、Enoki Ryosuke、Nakamura Takahiro J.、Mieda Michihiro
  • Journal Title: Proceedings of the National Academy of Sciences (PNAS) Volume: 118 Pages: 1-18
  • DOI: 10.1073/pnas.2010168118
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Electrophysiological Approaches to Studying the Suprachiasmatic Nucleus (2021)
  • Author(s): Michel S, Nakamura TJ, Meijer JH, Colwell CS.
  • Journal Title: Methods Mol Biol . Volume: 2130 Pages: 303-324
  • DOI: 10.1007/978-1-0716-0381-9_23
• [Journal Article] Double recording system of Period1 gene expression rhythm in the olfactory bulb and liver in freely moving mouse (2020)
  • Author(s): Hamada K, Oota A, Ito R, Kasahara S,Nakajima K, Kikuchi K, Sutherland K, Ishikawa M，Shirato H, Ozaki M, Hamada T
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 529 Pages: 898-903
  • DOI: 10.1016/j.bbrc.2020.05.224
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mouse period1 gene expression recording from olfactory bulb under free moving conditions with a portable optic fiber device (2020)
  • Author(s): Ito R, Hamada K, Kasahara S, Kikuchi Y, Nakajima K, Sutherland K, Shirato H, Ozaki M, Ishikawa M, Hamada T
  • Journal Title: Luminescence Volume: 35 Pages: 1248-1253
  • DOI: 10.1002/bio.3884
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 一個体の自由行動マウスの中枢および末梢深部組織の時計遺伝子Period1 発現の 同時計測システムの確立 (2020)
  • Author(s): 浜田俊幸
  • Journal Title: 未病と老化 博慈会 老人病研究所 Volume: Vol.29
• [Journal Article] 生体リズムの乱れる過程を可視化する計測システムの開発 (2020)
  • Author(s): 伊藤遼河, 浜田和子, 浜田俊幸
  • Journal Title: 地域ケアリング・北隆館 Volume: Vol.22, No.11
  • NAID: 40022639447
• [Journal Article] 生体リズムの乱れを超高感度で検出する計測システムの開発～疾患発症解明の 応用を目指して～ (2020)
  • Author(s): 伊藤遼河, 浜田和子, 浜田俊幸
  • Journal Title: 細胞・ ニューサイエンス社 Volume: Vol.52, No.10
• [Journal Article] 生体リズムの乱れを超高感度で検出する計測システムの開発～疾患発症解明への応用を目指して～ (2020)
  • Author(s): 伊藤遼河, 浜田和子, 浜田俊幸
  • Journal Title: アグリバイオ・北隆館 Volume: Vol.4, No.8
  • NAID: 40022661482
• [Journal Article] Sample preparation method with ultrafiltration for whole blood thiosulfate measurement (2020)
  • Author(s): Jin Shigeki、Murakami Manabu、Matoba Kotaro、Matoba Tomoko、Haga Sanae、Ozaki Michitaka、Takeuchi Akiko、Hyodoh Hideki
  • Journal Title: Leg. Med. Volume: 47 Pages: 101765-101765
  • DOI: 10.1016/j.legalmed.2020.101765
  • Peer Reviewed
• [Journal Article] Cellular and molecular mechanisms of liver regeneration: proliferation, growth, death and protection of hepatocytes (2020)
  • Author(s): Michitaka Ozaki
  • Journal Title: Semin Cell Dev Biol. Volume: 100 Pages: 62-73
  • DOI: 10.1016/j.semcdb.2019.10.007
  • Int'l Joint Research
• [Journal Article] Modeling circadian regulation of ovulation timing: age-related disruption of estrous cyclicity (2020)
  • Author(s): Ohara Takayuki、Nakamura Takahiro J.、Nakamura Wataru、Tokuda Isao T.
  • Journal Title: Sci Rep. Volume: 10 Pages: 16767-16767
  • DOI: 10.1038/s41598-020-73669-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reciprocal Expression Patterns of Placental Leucine Aminopeptidase/Insulin-Regulated Aminopeptidase and Vasopressin in the Murine Brain (2020)
  • Author(s): Goto Yoshikuni、Nakamura Takahiro J.、Ogawa Kenji、Hattori Akira、Tsujimoto Masafumi
  • Journal Title: Front Mol Biosci. Volume: 7 Pages: 168-168
  • DOI: 10.3389/fmolb.2020.00168
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A high-salt/high fat diet alters circadian locomotor activity and glucocorticoid synthesis in mice (2020)
  • Author(s): Yokoyama Yoko、Nakamura Takahiro J.、Yoshimoto Karen、Ijyuin Honoka、Tachikawa Naoyuki、Oda Haruka、Shiraishi Rena、Shinohara Kaori、Kumadaki Kayo、Honda Shiori、Nakamura Anna、Kitamura Naho、Tsubota Kazuo、Watanabe Mitsuhiro
  • Journal Title: PLoS One. Volume: 15
  • DOI: 10.1371/journal.pone.0233386
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Secretin receptor-deficient mice exhibit altered circadian rhythm in wheel-running activity (2020)
  • Author(s): Sugiyama Mizuki、Nishijima Ichiko、Miyazaki Shota、Nakamura Takahiro J.
  • Journal Title: Neuroscience Letters Volume: 722 Pages: 134814-134814
  • DOI: 10.1016/j.neulet.2020.134814
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Validation of Fully Automatic Quantitative Software for Finger Joint Space Narrowing Progression for Rheumatoid Arthritis Patients. (2020)
  • Author(s): Narisawa C, Sutherland K, Lu Y, Furusaki A, Sagawa A, Kamishima T.
  • Journal Title: J Digit Imaging. Volume: 33 Pages: 1387-1392
  • DOI: 10.1007/s10278-020-00390-6
  • Peer Reviewed / Open Access
• [Journal Article] Fully automatic quantitative software for assessment of minute finger joint space narrowing progression on radiographs: evaluation in rheumatoid arthritis patients with long-term sustained clinical low disease activity. (2020)
  • Author(s): Kato K, Sutherland K, Tanaka Y, Kato M, Fukae J, Tanimura K, Kamishima T.
  • Journal Title: Japanese Journal of Radiology Volume: 38 Issue: 10 Pages: 979-986
  • DOI: 10.1007/s11604-020-00996-4
  • NAID: 120007042002
  • ISSN: 1867-1071, 1867-108X
  • Peer Reviewed
• [Journal Article] Convolutional neural network for classification of two-dimensional array images generated from clinical information may support diagnosis of rheumatoid arthritis. (2020)
  • Author(s): Fukae J, Isobe M, Hattori T, Fujieda Y, Kono M, Abe N, Kitano A, Narita A, Henmi M, Sakamoto F, Aoki Y, Ito T, Mitsuzaki A, Matsuhashi M, Shimizu M, Tanimura K, Sutherland K, Kamishima T, Atsumi T, Koike T.
  • Journal Title: Sci Rep. Volume: 10 Pages: 5648-5648
  • DOI: 10.1038/s41598-020-62634-3
  • Peer Reviewed / Open Access
• [Journal Article] Quick and accurate selection of hand images among radiographs from various body parts using deep learning. (2020)
  • Author(s): Fujiwara K, Fang W, Okino T, Sutherland K, Furusaki A, Sagawa A, Kamishima T.
  • Journal Title: J Xray Sci Technol. Volume: 28 Pages: 1199-1206
  • DOI: 10.3233/xst-200694
  • Peer Reviewed
• [Journal Article] Characterization of the circadian oscillator in the choroid plexus of rats (2020)
  • Author(s): Yamaguchi Takeshi、Hamada Toshiyuki、Matsuzaki Toshiyuki、Iijima Norio
  • Journal Title: Biochem Biophys Res Commun. Volume: 524 Pages: 497-501
  • DOI: 10.1016/j.bbrc.2020.01.125
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Secretin receptor-deficient mice exhibit altered circadian rhythm in wheel-running activity (2020)
  • Author(s): Sugiyama Mizuki、Nishijima Ichiko、Miyazaki Shota、Nakamura Takahiro J.
  • Journal Title: Neuroscience Letters Volume: 772 Pages: 134814-134814
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Detection of Fine Radiographic Progression in Finger Joint Space Narrowing Beyond Human Eyes: Phantom Experiment and Clinical Study with Rheumatoid Arthritis Patients. (2019)
  • Author(s): Kato K, Yasojima N, Tamura K, Ichikawa S, Sutherland K, Kato M, Fukae J, Tanimura K, Tanaka Y, Okino T, Lu Y, Kamishima T.
  • Journal Title: Sci Rep. Volume: 9 Pages: 8526-8526
  • DOI: 10.1038/s41598-019-44747-6
  • Peer Reviewed / Open Access
• [Journal Article] Quantitative power Doppler signal assessment in the subchondral bone region of the metacarpophalangeal joint is an effective predictor of radiographic progression in the hand of rheumatoid arthritis: a pilot study. (2019)
  • Author(s): Fujimori M, Kamishima T, Narita A, Henmi M, Kato M, Sutherland K, Nishida M, Tanaka Y, Yutong L, Tanimura K, Atsumi T.
  • Journal Title: Rheumatol Int. Volume: 39 Pages: 1413-1421
  • DOI: 10.1007/s00296-019-04320-w
  • Peer Reviewed
• [Journal Article] Intravoxel incoherent motion MRI for discrimination of synovial proliferation in the hand arthritis: A prospective proof-of-concept study. (2019)
  • Author(s): Fujimori M, Murakami K, Sugimori H, Lu Y, Sutherland K, Oki N, Aoki T, Kamishima T.
  • Journal Title: J Magn Reson Imaging. Volume: 50 Pages: 1199-1206
  • DOI: 10.1002/jmri.26660
  • Peer Reviewed
• [Journal Article] 3D Transformation Matrix Calculation and Pixel Intensity Normalization for the Dual Focus Tracking System (2019)
  • Author(s): Kennth Sutherland, Toshiyuki Hamada*, Masayori Ishikawa*, Naoki Miyamoto, MasahiroMizuta, Hiroyuki Date, Hiroki Shirato ( * Correspondence)
  • Journal Title: Journal of Medical and Biological Engineering Volume: 39 Pages: 952-959
  • DOI: 10.1007/s40846-019-00474-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Type XVII collagen coordinates proliferation in the interfollicular epidermis. (2019)
  • Author(s): Watanabe M, Natsuga K, Nishie W, Kobayashi Y, Donati G, Suzuk Si, Fujimura Y, Tsukiyama T, Ujiie H, Shinkuma S, Nakamura H, Murakami M, Ozaki M, Nagayama M, Watt F and Shimizu H
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 6:e26635 Pages: 2110-2120
  • DOI: 10.7554/elife.26635
  • NAID: 120006337772
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Activation of caspase-3 during Chlamydia trachomatis-induced apoptosis at a late stage (2019)
  • Author(s): Matsuo Junji、Haga Sanae、Hashimoto Kent、Okubo Torahiko、Ozawa Takeaki、Ozaki Michitaka、Yamaguchi Hiroyuki
  • Journal Title: Can. J. Microbiol. Volume: 65 Pages: 135-143
  • DOI: 10.1139/cjm-2018-0408
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Suppression of Blue Light at Night Ameliorates Metabolic Abnormalities by Controlling Circadian Rhythms (2019)
  • Author(s): Nagai Norihiro、Ayaki Masahiko、Yanagawa Tatsuo、Hattori Atsuhiko、Negishi Kazuno、Mori Takuro、Nakamura Takahiro J.、Tsubota Kazuo
  • Journal Title: Investigative Opthalmology & Visual Science Volume: 60 Pages: 3786-3786
  • DOI: 10.1167/iovs.19-27195
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of testosterone on circadian rhythmicity in old mice (2019)
  • Author(s): Atsuyoshi Hashimoto, Shingo Fujiki, Wataru Nakamura & Takahiro J. Nakamura
  • Journal Title: J Physiol Sci. Volume: 69 Pages: 791-798
  • DOI: 10.1007/s12576-019-00695-4
  • NAID: 40022006474
  • Peer Reviewed / Open Access
• [Journal Article] Photo-Activatable Akt Probe: A New Tool to Study the Akt-Dependent Physiopathology of Cancer Cells. (2018)
  • Author(s): Haga, Sanae; Ozawa, Takeaki; Morita, Naoki; Asano, Mami; Jin, Shigeki; Yimin; Ozaki, Michitaka.
  • Journal Title: Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics Volume: 26(3) Pages: 467-472
  • NAID: 120006473610
  • Peer Reviewed
• [Journal Article] Detection of Necroptosis in Ligand-Mediated and Hypoxia-Induced Injury of Hepatocytes Using a Novel Optic Probe-Detecting Receptor-Interacting Protein (RIP)1/RIP3 Binding (2018)
  • Author(s): Haga, Sanae; Kanno, Akira; Ozawa, Takeaki; Morita, Naoki; Asano, Mami; Ozaki, Michitaka
  • Journal Title: Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics Volume: 26 Pages: 503-513
  • NAID: 120006473611
  • Peer Reviewed
• [Journal Article] The central clock controls the daily rhythm of Aqp5 expression in salivary glands. (2018)
  • Author(s): Uchida H, Nakamura TJ, Takasu NN, Obana-Koshino A, Ono H, Todo T, Sakai T, Nakamura W
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  • DOI: 10.1007/s12576-017-0540-1
  • NAID: 40021574351
  • Peer Reviewed
• [Journal Article] 光技術を用いた臓器・細胞機能評価と制御 (2017)
  • Author(s): 尾崎倫孝、芳賀 早苗、小澤岳昌、森田直樹、浜田俊幸
  • Journal Title: Organ Biology Volume: 24 Pages: 87-91
  • Peer Reviewed
• [Journal Article] Semi-Automated Quantification of Finger Joint Space Narrowing Using Tomosynthesis in Patients with Rheumatoid Arthritis (2017)
  • Author(s): Ichikawa S, Kamishima T, Sutherland K, Kasahara H, Shimizu Y, Fujimori M, Yasojima N, Ono Y, Kaneda T, Koike T
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  • DOI: 10.1007/s10278-017-9949-6
  • Peer Reviewed
• [Journal Article] Pre-operative volume rather than area of skeletal muscle is a better predictor for post-operative risks for respiratory complications in living-donor liver transplantation. (2017)
  • Author(s): Wada Y, Kamishima T, Shimamura T, Kawamura N, Yamashita K, Sutherland K, Takeda H
  • Journal Title: Br J Radiol Volume: 1072 Pages: 20160938-20160938
  • DOI: 10.1259/bjr.20160938
  • Peer Reviewed
• [Journal Article] Computer-Based Radiographic Quantification of Joint Space Narrowing Progression Using Sequential Hand Radiographs: Validation Study in Rheumatoid Arthritis Patients from Multiple Institutions (2017)
  • Author(s): Ichikawa S, Kamishima T, Sutherland K, Fukae J, Katayama K, Aoki Y, Okubo T, Okino T, Kaneda T, Takagi S, Tanimura K
  • Journal Title: J Digit Imaging Volume: 30(5) Pages: 648-656
  • DOI: 10.1007/s10278-017-9970-9
  • Peer Reviewed
• [Journal Article] Cartilage quantification using contrast-enhanced MRI in the wrist of rheumatoid arthritis: cartilage loss is associated with bone marrow edema (2017)
  • Author(s): Fujimori M, Nakamura S, Hasegawa K, Ikeno K, Ichikawa S, Sutherland K, Kamishima T.
  • Journal Title: Br J Radiol Volume: 90(1077) Pages: 20170167-20170167
  • DOI: 10.1259/bjr.20170167
  • Peer Reviewed
• [Journal Article] Visceral adipose tissue area measurement at a single level: can it represent visceral adipose tissue volume? (2017)
  • Author(s): Noumura Y, Kamishima T, Sutherland K, Nishimura H
  • Journal Title: Br J Radiol Volume: 90(1077) Pages: 20170253-20170253
  • DOI: 10.1259/bjr.20170253
  • Peer Reviewed
• [Journal Article] Relevance of FXR-p62/SQSTM1 pathway for survival and protection of mouse hepatocytes and liver, especially with steatosis. (2017)
  • Author(s): Sanae Haga, Yimin, Michitaka Ozaki.
  • Journal Title: BMC Gastroenterol. Volume: 17 Pages: 9-9
  • DOI: 10.1186/s12876-016-0568-3
  • NAID: 120005981455
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Age-related circadian disorganization caused by sympathetic dysfunction in peripheral clock regulation (2017)
  • Author(s): Tahara Y, Takatsu Y, Shiraishi T, Kikuchi Y, Yamazaki M, Motohashi H, Muto A, Sasaki H, Haraguchi A, Kuriki D, Nakamura TJ, Shibata S
  • Journal Title: Npj Aging and Mechanisms of Disease Volume: 3 Pages: 16030-16030
  • DOI: 10.1038/npjamd.2016.30
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 自由行動マウスの複数組織における時計遺伝子発現のin vivo追跡定量化 (2017)
  • Author(s): 浜田俊幸
  • Journal Title: 未病と抗老化 Volume: 26 Pages: 82-82
• [Presentation] Real time recording of Period1 gene in the central and peripheral tissues of the freely moving streptozotocin-treated diabetic mouse (2021)
  • Author(s): Kouki Nagasawa, Yukina Yoshida, Ryousuke Sato, Mizuki Nakaya, Juri Hayashi, Yuki Ishi, Masayori Ishikawa, Mitchitaka Ozaki, Kazuko Hamada, Toshiyuki Hamada
  • Organizer: 第94回 日本薬理学会年会
• [Presentation] The analysis of Period1 gene expression in freely moving mouse using micro PMT system (2021)
  • Author(s): Yuki Ishii, Juri Hayashi, Mizuki Nakaya, Rousuke Sato, Yukina Yoshida, Kouki Nagasawa, Masayori Ishikawa, Mitchitaka Ozaki, Kazuko Hamada, Toshiyuki Hamada
  • Organizer: 第94回 日本薬理学会年会
• [Presentation] administration of methamphetamine on circadian rhythm of mice (2021)
  • Author(s): Mizuki Nakaya, Ryousuke Sato, Kouki Nagasawa, Yukina Yoshida , Juri Hayashi, Yuki Ishi, Masayori Ishikawa, Mitchitaka Ozaki, Kazuko Hamada, Toshiyuki Hamada
  • Organizer: 第94回 日本薬理学会年会
• [Presentation] 生体リズムが乱れる過程をリアルタイムに解析するシステムの構築 ～病態モデルマウスを用いての検討～ (2020)
  • Author(s): 長沢光樹,石井悠暉, 林珠理, 吉田幸那, 狩野晴美, 石川正純, 尾崎倫孝, 浜田和子, 浜田俊幸
  • Organizer: 第64回日本薬学会 関東支部大会
• [Presentation] 時計遺伝子発現定量解析を用いた糖尿病発症機構解明 (2020)
  • Author(s): 石井悠暉, 長沢光樹, 林珠理, 吉田幸那, 狩野晴美, 石川正純, 尾崎倫孝, 浜田和子, 浜田俊幸
  • Organizer: 第64回日本薬学会 関東支部大会
• [Presentation] 覚せい剤による生体リズムの乱れを毛1本から推測するシステムの開発 (2020)
  • Author(s): 浜田俊幸
  • Organizer: 第10回 国際医療福祉大学学会学術大会
• [Presentation] Anesthetic effects on various clocks in the rat brain (2020)
  • Author(s): 山口剛史、浜田俊幸、飯島典生
  • Organizer: 第27回日本生物学会学術大会
• [Presentation] Poly(ADP-ribose) polymerase plays a critical role in oxidative stress- and hypoxia/reoxygenation-induced programmed cell death in mouse liver cells. (2020)
  • Author(s): Michitaka Ozaki, Sanae Haga, Mami Asano, Naoki Morita.
  • Organizer: The Digital International Liver Congress 2020
  • Int'l Joint Research
• [Presentation] 「肝臓疾患モデルについて」研究セミナー FBRA研究会 (2020)
  • Author(s): 尾崎倫孝
  • Organizer: 玄米酵素（株）本社4階会議室、札幌
• [Presentation] 「PARP が関与する肝傷害の意義」 第32回日本肝臓医生物学研究会；LBSG-J（プロメテウスの会） (2020)
  • Author(s): 尾崎倫孝
  • Organizer: 東京医科歯科大学 M&D タワー21階 大学院講義室
• [Presentation] 「肝虚血再灌流傷害進展における Poly（ADP-ribose）polymerase（PARP）依存性細胞死の解析」 (2020)
  • Author(s): 尾崎倫孝、芳賀早苗、浅野真未、菅野憲、小澤岳昌、森田直樹
  • Organizer: 第27回肝細胞研究会（デジタル開催）
• [Presentation] 「様々な様式の細胞死は、慢性脂肪肝における遷延化した組織傷害に関与する可能性がある」 (2020)
  • Author(s): 芳賀早苗、森田直樹、小澤岳昌、尾崎倫孝
  • Organizer: 第93回日本生化学会大会
• [Presentation] 「視交叉上核隔離が概日リズムに与える影響」 (2020)
  • Author(s): 宮﨑翔太、前嶋大智、水田習斗、中村孝博
  • Organizer: 第15回環境生理学プレコングレス
• [Presentation] 「雌性生殖におけるAVPニューロンのGABAの役割」 (2020)
  • Author(s): 陳佳旭、杉山瑞樹、三枝理博、中村孝博
  • Organizer: 第27回日本時間生物学会学術大会
• [Presentation] 「セクレチン受容体欠損マウスにおける給餌予知活動」 (2020)
  • Author(s): 杉山瑞樹、西島維知子、中村渉、中村孝博
  • Organizer: 第27回日本時間生物学会学術大会
• [Presentation] 第27回日本時間生物学会学術大会 (2020)
  • Author(s): 宮﨑翔太、中村渉、中村孝博
  • Organizer: 「視交叉上核隔離がマウス糞中コルチコステロンリズムに与える影響」
• [Presentation] Effects of the isolation of suprachiasmatic nucleus on circadian rhythmicity. (2020)
  • Author(s): Shota Miyazaki, Daichi Maejima, Shuto Mizuta, Wataru Nakamura, Takahiro J. Nakamura
  • Organizer: SRBR 2020 Society for Research on Biological Rhythms
  • Int'l Joint Research
• [Presentation] Roles of GABA in suprachiasmatic AVP Neurons on Female Reproductive Function. (2020)
  • Author(s): Jiaxu Chen, Mizuki Sugiyama, Michihiro Mieda, Takahiro J. Nakamura
  • Organizer: SRBR 2020 Society for Research on Biological Rhythms
  • Int'l Joint Research
• [Presentation] Circadian Rhythms of Triglyceride Accumulation in Adipocytes. (2020)
  • Author(s): Rena Shiraishi, Satomi Morita, Yoshikuni Goto, Hikari Yoshitane, Yoshimasa Asano, Yoshitaka Fukada, Yasushi Mizoguchi, Takahiro J. Nakamura
  • Organizer: SRBR 2020 Society for Research on Biological Rhythms
  • Int'l Joint Research
• [Presentation] The circadian clock in mammals (2020)
  • Author(s): Takahiro J. Nakamura, Nana N. Takasu, Wataru Nakamura
  • Organizer: ICMMA2019 International Conference on "Spatio-temporal patterns on various levels of the hierarchy of life"
  • Int'l Joint Research
• [Presentation] 「自由行動マウスの長期間遺伝子発現を検出するための機器製作」 (2020)
  • Author(s): 千島綾、狩野晴美、太田あかり、中島かな子、菊池祥裕、伊藤遼河、石川正純、尾崎倫孝、浜田和子、浜田俊幸
  • Organizer: 日本薬学会 第140年会
• [Presentation] 「In vivo 長期間遺伝子発現定量解析におけるL-ルシフェリンのルシフェリン-ルシフェラーゼ反応阻害効果」 (2020)
  • Author(s): 中島かな子, 伊藤遼河, 菊池祥裕, 狩野晴美, 千島綾, 太田あかり, 石川正純, 尾崎倫孝, 浜田和子, 浜田俊幸
  • Organizer: 日本薬学会 第140年会
• [Presentation] 「PMT埋め込みマウスの長期間遺伝子発現解析」 (2020)
  • Author(s): 菊池祥裕, 中島かな子, 狩野晴美, 千島綾, 太田あかり, 伊藤遼河, 石川正純, 尾崎倫孝, 浜田和子, 浜田俊幸
  • Organizer: 日本薬学会 第140年会
• [Presentation] 「自由行動マウスの中枢と末梢組織の遺伝子発現を長期間同時計測するシステム開発 」 (2020)
  • Author(s): 伊藤遼河, 菊池祥裕, 中島かな子, 狩野晴美, 太田あかり,千島綾, 石川正純, 尾崎倫孝, 浜田和子, 浜田俊幸
  • Organizer: 日本薬学会 第140年会
• [Presentation] 「サーカディアンリズム同調と性周期 (2019)
  • Author(s): 中村渉、中村孝博、織田善晃、高須奈々
  • Organizer: 第26回日本時間生物学会学術大会
• [Presentation] 「体内時計が制御する睡眠覚醒リズムと加齢に伴う機能低下」 (2019)
  • Author(s): 中村渉、中村孝博、高須奈々
  • Organizer: 「体内時計が制御する睡眠覚醒リズムと加齢に伴う機能低下」
• [Presentation] Effects of the isolation of suprachiasmatic nucleus on circadian rhythmicity (2019)
  • Author(s): Shota Miyazaki, Daichi Maejima, Shuto Mizuta, Wataru Nakamura, Takahiro J. Nakamura
  • Organizer: 16th Congress of the European Biological Rhythms Society
  • Int'l Joint Research
• [Presentation] Time-restricted feeding is effective in the BACHD mouse model of Huntington’s disease (2019)
  • Author(s): Yu Tahara, Daniel S. Whittaker, Dawn H. Loh, Huei-Bin Wang, Christopher S.H. Luk, Dika Kuljis, Takahiro J. Nakamura, Shigenobu Shibata, Gene D. Block, Christopher S. Colwell
  • Organizer: 16th Congress of the European Biological Rhythms Society, Lyon
  • Int'l Joint Research
• [Presentation] In vivo monitoring reveals two different oscillators in methamphetamine treated mice (2019)
  • Author(s): Shota Miyazaki, Yu Tahara, Christopher S. Colwell, Gene D. Block, Wataru Nakamura, Takahiro J. Nakamura,
  • Organizer: ICMMA2019 International Conference on "Spatio-temporal patterns on various levels of the hierarchy of life"
  • Int'l Joint Research
• [Presentation] Roles of GABA in Suprachiasmatic AVP Neurons on Female Reproductive Functions (2019)
  • Author(s): Mizuki Sugiyama, Michihiro Mieda, Takahiro J. Nakamura
  • Organizer: ICMMA2019 International Conference on "Spatio-temporal patterns on various levels of the hierarchy of life"
  • Int'l Joint Research
• [Presentation] Effects of the isolation of suprachiasmatic nucleus on rhythms of body temperature and peripheral clocks (2019)
  • Author(s): Daichi Maejima, Shota Miyazaki, Shuto Mizuta, Wataru Nakamura, Takahiro J. Nakamura
  • Organizer: ICMMA2019 International Conference on "Spatio-temporal patterns on various levels of the hierarchy of life"
  • Int'l Joint Research
• [Presentation] 時間給餌によるハンチントンモデル病モデルマウスの疾患予防効果」 (2019)
  • Author(s): 田原優、Daniel S. Whittaker, Dawn H. Loh, Huei-Bin Wang, Christopher SH.Luk, Dika Kujis ,中村孝博、Gene D. Block, Christopher S. Colwell
  • Organizer: 第26回日本時間生物学会学術大会
• [Presentation] 「視交叉上核隔離が概日リズムに与える影響」 (2019)
  • Author(s): 宮崎翔太、前嶋大智、水田習斗、中村孝博
  • Organizer: 第26回日本時間生物学会学術大会
• [Presentation] 「雌性生殖機能に対する概日リズムストラテジー」 (2019)
  • Author(s): 坂詰小百合、中村渉、中村孝博
  • Organizer: 第26回日本時間生物学会学術大会
• [Presentation] 「雌性生殖機能における視交叉上核AVP細胞特異的GABAの役割 (2019)
  • Author(s): 杉山瑞樹、三枝理博、中村孝博
  • Organizer: 第26回日本時間生物学会学術大
• [Presentation] 「排卵タイミングの概日制御に関する数理モデル」 (2019)
  • Author(s): 大原隆之、中村孝博、中村渉、徳田功
  • Organizer: 第26回日本時間生物学会学術大会
• [Presentation] 光触媒窒化タンタルの細胞毒性と細胞時計への影響 (2019)
  • Author(s): 立石真由、渡邉友亮、中村孝博
  • Organizer: 第26回日本時間生物学会学術大会
• [Presentation] 「時間栄養学による神経変性疾患の予防」 (2019)
  • Author(s): 田原優、Daniel S. Whittaker, Dawn H. Loh, Huei-Bin Wang, Christopher SH. Luk, Dika Kujis, 中村孝博、Gene D. Block, Christopher S. Colwell
  • Organizer: 第6回時間栄養科学研究会
• [Presentation] Roles of GABA in Suprachiasmatic AVP Neurons on Female Reproductive Functions (2019)
  • Author(s): Mizuki Sugiyama, Michihiro Mieda, Takahiro J. Nakamura
  • Organizer: 16th Congress of the European Biological Rhythms Society, Lyon
  • Int'l Joint Research
• [Presentation] Modeling circadian regulation of ovulation timing: Age-related disruption of estrous cyclicity (2019)
  • Author(s): Takayuki Ohara, Takahiro J. Nakamura, Wataru Nakamura, Isao T. Tokuda
  • Organizer: 16th Congress of the European Biological Rhythms Society, Lyon
  • Int'l Joint Research
• [Presentation] Effects of the isolation of suprachiasmatic nucleus on circadian rhythmicity (2019)
  • Author(s): Shota Miyazaki, Daichi Maejima, Shuto Mizuta, Wataru Nakamura, Takahiro J. Nakamura
  • Organizer: V World Congress of Chronobiology
  • Int'l Joint Research
• [Presentation] 「生体リズムの乱れを超高感度で検出する組織密着型センサーの開発 ～疾患発症解明への応用を目指して～」 (2019)
  • Author(s): 狩野晴美, 伊藤遼河, 浜田和子, 菊池祥裕, 中島かな子, 石川正純, 尾崎 倫孝, 浜田俊幸
  • Organizer: 第26回日本時間生物学会学術大会
• [Presentation] 「生体リズムの乱れを超高感度で検出する組織密着型センサーシステムの開発」 (2019)
  • Author(s): 浜田俊幸
  • Organizer: 第11回トランスポーター研究会九州部会
  • Invited
• [Presentation] 生物時計の発振と同調機構にみられる共通性と多様性 シンポジウム 「新規遺伝子発現定量イメージング技術をもちいた自由行動マウスの時計遺伝子発現リズム」 (2019)
  • Author(s): 浜田俊幸
  • Organizer: 第26回 日本時間生物学会
  • Invited
• [Presentation] 「自由行動マウス生体深部の遺伝子発現を長期間定量解析する」 (2019)
  • Author(s): 浜田俊幸
  • Organizer: 文部科学省科研費新学術Resonance Bio全体会議2019 Buy Me! Discover Resonance in the CHAOS
• [Presentation] 「リアルタイムに乳癌を検出する組織密着型センサーシステムの開発と応用」 (2019)
  • Author(s): 浜田俊幸
  • Organizer: 晝馬輝夫 光科学賞の授与及び平成30年度研究助成金贈呈の式典
  • Invited
• [Presentation] Panelist, MathWorkMathWorks (2019)
  • Author(s): Hamada Toshiyuki
  • Organizer: MathWorks Research Summit 2019 Asia
  • Int'l Joint Research / Invited
• [Presentation] 「Real time recording of clock gene expression in multiple tissues of freely moving mice」 (2019)
  • Author(s): Hamada K, Ito R, Kikuchi Y, Nakagjima K,Ishikawa M, Ozaki M, Hamada T
  • Organizer: Resonance Bio International Symposium
  • Int'l Joint Research
• [Presentation] 「Real time recording of clock gene expression in peripheral tissues of freely moving mice」 (2019)
  • Author(s): Hamada K, Ito R, Kikuchi Y, Nakagjima K, Hamada T
  • Organizer: XVI European Biological Rhythms socitety Congress
  • Int'l Joint Research
• [Presentation] 「Real time recording of clock gene expression in multiple tissues of freely moving mice」 (2019)
  • Author(s): Hamada K, Kenneth Sutherland K, Ishikawa M, Hamada T
  • Organizer: 9th Federation of the Asian and Oceanian Physiological Societies Congress
  • Int'l Joint Research
• [Presentation] 「肝傷害および進展におけるPoly(ADP-ribose) polymerase-1(PARP1) の役割」 (2019)
  • Author(s): 尾崎倫孝
  • Organizer: 第31回日本肝臓医生物学研究会LBSG-J（プロメテウスの会）
• [Presentation] 「肝虚血・再灌流、酸化ストレスと細胞死（臓器傷害）とその制御 -マウス肝における虚血再灌流傷害進展の分子機構 -」シンポジウム１「酸素が糾う病態と疾患 -細胞死・傷害、炎症、再生、代謝-」 (2019)
  • Author(s): 尾崎倫孝、芳賀 早苗、森田 直樹、小澤 岳昌
  • Organizer: 第72回日本酸化ストレス学会学術集会
• [Presentation] 「Fasリガンド/酸化ストレスによって引き起こされるプログラム細胞死の機序解析 Analysis of programmed cell death in hepatocytes induced by Fas ligand and oxidative stress」 (2019)
  • Author(s): 芳賀早苗、森田直樹、尾崎倫孝
  • Organizer: 第92回日本生化学会総会
• [Presentation] 「脂肪肝における易傷害性メカニズム解析の基礎的研究」 (2019)
  • Author(s): 芳賀早苗、浅野真未、森田直樹、尾崎倫孝
  • Organizer: 第26回肝細胞研究会
• [Presentation] 「脳内メトアンフェタミン概日振動体の観察」 (2019)
  • Author(s): 宮崎翔太、田原優、ColwellChristopher S、Block Gene D、中村渉、中村孝博
  • Organizer: 第97回日本生理学会大会
• [Presentation] The circadian clock in mammals (2019)
  • Author(s): Takahiro J. Nakamura, Nana N. Takasu, Wataru Nakamura
  • Organizer: ICMMA2019 International Conference on "Spatio-temporal patterns on various levels of the hierarchy of life"
  • Int'l Joint Research
• [Presentation] In vivo monitoring reveals two different oscillators in methamphetamine treated mice (2019)
  • Author(s): Shota Miyazaki, Yu Tahara, Christopher S. Colwell, Gene D. Block, Wataru Nakamura, Takahiro J. Nakamura
  • Organizer: ICMMA2019 International Conference on "Spatio-temporal patterns on various levels of the hierarchy of life"
  • Int'l Joint Research
• [Presentation] Circadian Strategy for Female Reproduction (2019)
  • Author(s): Sayuri Sakazume, Wataru Nakamura, Takahiro J. Nakamura
  • Organizer: ICMMA2019 International Conference on "Spatio-temporal patterns on various levels of the hierarchy of life"
  • Int'l Joint Research
• [Presentation] 「サーカディアンリズム同調と性周期」 (2019)
  • Author(s): 中村渉、中村孝博、織田善晃、高須奈々
  • Organizer: 第26回日本時間生物学会学術大会
  • Invited
• [Presentation] Effects of the isolation of suprachiasmatic nucleus on circadian rhythmicity (2019)
  • Author(s): Shota Miyazaki, Daichi Maejima, Shuto Mizuta, Wataru Nakamura, Takahiro J. Nakamura,
  • Organizer: 16th Congress of the European Biological Rhythms Society
  • Int'l Joint Research
• [Presentation] Roles of GABA in Suprachiasmatic AVP Neurons on Female Reproductive Functions (2019)
  • Author(s): Mizuki Sugiyama, Michihiro Mieda, Takahiro J. Nakamura,
  • Organizer: 16th Congress of the European Biological Rhythms Society, Lyon
• [Presentation] Time-restricted feeding is effective in the BACHD mouse model of Huntington’s disease (2019)
  • Author(s): Yu Tahara, Daniel S. Whittaker, Dawn H. Loh, Huei-Bin Wang, Christopher S.H. Luk, Dika Kuljis, Takahiro J. Nakamura, Shigenobu Shibata, Gene D. Block, Christopher S. Colwell
  • Organizer: 16th Congress of the European Biological Rhythms Society
  • Int'l Joint Research
• [Presentation] 「体内時計が制御する睡眠覚醒リズムと加齢に伴う機能低下」 (2019)
  • Author(s): 中村渉、中村孝博、高須奈々
  • Organizer: 日本睡眠学会第44回定期学術集会
  • Invited
• [Presentation] In vivo imaging of clock gene expression in multiple tissues of freely moving mice (2017)
  • Author(s): 浜田俊幸
  • Organizer: 第94回日本生理学会大会
  • Invited
• [Presentation] iPRECIOをもちいた自由行動マウスの全身遺伝子発現の可視化と定量化 (2017)
  • Author(s): 浜田俊幸
  • Organizer: 第40回日本神経科学大会 ランチョンセミナー
  • Invited
• [Presentation] 生体複数部位の遺伝子発現を長期間追跡定量する新規光計測システム (2017)
  • Author(s): 浜田俊幸
  • Organizer: 第３回可視化マウス研究会
• [Presentation] Type XVII collagen suppresses interfollicular epidermal proliferation in neonatal and aged skin, and helps rejuvenate epidermis. (2017)
  • Author(s): Mika Watanabe, Ken Natsuga, Wataru Nishie, Giacomo Donati, Yu Fujimura, Tadasuke Tsukiyama, Hideyuki Ujiie, Michitaka Ozaki, Fiona M Watt, and Hiroshi Shimizu
  • Organizer: 47th Annual ESDR Meeting Salzburg
  • Int'l Joint Research
• [Presentation] Type XVII collagen regulates proliferation in the interfollicular epidermis (2017)
  • Author(s): Mika Watanabe, Ken Natsuga, Yasuaki Kobayashi, Wataru Nishie, Giacomo Donati, Shotaro Suzuki, Yu Fujimura, Tadasuke Tsukiyama, Hideyuki Ujiie, Satoru Shinkuma, Masamoto Murakami, Michitaka Ozaki, Masaharu Nagayama, Fiona. M Watt, Hiroshi Shimizu
  • Organizer: 日本研究皮膚科学会 第42回年次学術大会・総会
• [Presentation] カスパーゼ3プローブ発現細胞を用いたクラミジア感染宿主細胞内のアポトーシス制御機構の探索 (2017)
  • Author(s): 松尾 淳司、芳賀 早苗、大久保 寅彦、中村 眞二、小澤 岳昌、尾崎 倫孝、山口 博之
  • Organizer: ConBio2017
• [Presentation] ビルベリー抽出物の糖尿病性網膜症発症・進行予防効果に関する基礎的研究 (2017)
  • Author(s): 浅野 真未、芳賀 早苗、柴崎 彩、黒澤 和也、荘厳 哲哉、尾崎 倫孝
  • Organizer: ConBio2017
• [Presentation] 肝細胞におけるレドックス依存性ネクロプトーシスの動態解析 (2017)
  • Author(s): 芳賀 早苗、菅野 憲、小澤 岳昌、森田 直樹、浅野 真未、伊 敏、尾崎 倫孝
  • Organizer: ConBio2017
• [Presentation] マウス脂肪化肝細胞・脂肪肝に対するビルベリーの抑制効果の検討 (2017)
  • Author(s): 芳賀 早苗、荘厳 哲哉、伊 敏、森田 直樹、浅野 真未、尾崎 倫孝
  • Organizer: 第38回日本肥満学会
• [Presentation] 肝核内受容体FXRはp62/SQSTM1およびSHPを経由して、それぞれ抗酸化・細胞保護効果、脂肪化抑制効果を示す (2017)
  • Author(s): 尾崎 倫孝、芳賀 早苗、伊 敏
  • Organizer: 第2４回肝細胞研究会
• [Presentation] 脂肪化肝細胞・脂肪肝に対するビルベリーの効果とその機序の検討 (2017)
  • Author(s): 芳賀 早苗、荘厳 哲哉、伊 敏、森田 直樹、浅野 真未、尾崎 倫孝
  • Organizer: 第2４回肝細胞研究会
• [Presentation] Circadian Disruption on the Female Reproductive System (2017)
  • Author(s): 中村孝博
  • Organizer: 第44回日本毒性学会学術年会
  • Invited
• [Presentation] Effects of the isolation of suprachiasmatic nucleus on circadian system (2017)
  • Author(s): 中村孝博
  • Organizer: 第95回日本生理学会大会
  • Invited
• [Presentation] Effects of the isolation of suprachasmatic nucleus on circadian wheel running activity (2017)
  • Author(s): Shuto Mizuta, Wataru Nakamura, Takahiro J. Nakamura
  • Organizer: 15th Congress of the European Biological Rhythms Society
• [Presentation] 加齢による生体リズムと皮膚機能への影響 (2017)
  • Author(s): 織田はるか、横山葉子、立川直之、中村孝博、渡辺光博
  • Organizer: 第17回日本抗加齢医学会総会
• [Presentation] Aging in Circadian Rhythms and Reproductive Functions (2017)
  • Author(s): 中村渉、高須奈々、中村孝博
  • Organizer: 第24回日本時間生物学会学術大会
• [Presentation] Molecular basis of lipid metabolism control by circadian clock (2017)
  • Author(s): 森田里美、後藤芳邦、吉種光、浅野吉政、深田吉孝、八木田和弘、中村孝博
  • Organizer: 第24回日本時間生物学会学術大会
• [Book] マウス肝におけるアポトーシス・イメージングの実際「発光イメージング実験ガイド 機能イメージングから細胞・組織・個体まで蛍光で観えないものを観る！」（永井健治、小澤岳昌編） (2019)
  • Author(s): 芳賀早苗、尾崎倫孝
  • Publisher: 羊土社
• [Book] 環境時刻と概日リズムとの調和による不妊解消戦略 (2019)
  • Author(s): 中村渉、中村孝博
  • Publisher: 実験医学
• [Remarks] Hamada Lab. 国際医療福祉大学 薬学部 年齢軸生命機能解析学分野
  • URL: https://hamada-lab.amebaownd.com/