Explore of unconventional microtubules
Project/Area Number |
17H04047
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General medical chemistry
|
Research Institution | Osaka City University |
Principal Investigator |
|
Project Period (FY) |
2017-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
|
Keywords | 細胞骨格 / 神経発生 / 神経変性疾患 / 細胞内物質輸送 / 神経細胞 / 中枢神経系 / 微小管 / 細胞質ダイニン / 神経科学 |
Outline of Final Research Achievements |
We demonstrated that αSyn-bound tMTs carrying 14pfs, was confirmed by immuno-TEM analysis. This is the first evidence that shows the distinctive function of MTs with unconventional pfs numbers. Although in vitro tubulin polymerization yields MTs comprising 15pfs, the MTs in mammalian cells were previously thought to exclusively contain 13-pfs 6. MTs carrying 14pfs yield a helical arrangement 60 that is unsuitable for kinesin1 movement. However, tMTs would not be used for regular cytoskeletal MTs, as they are too small to provide a trafficking rail for motor proteins. Rather, tMTs are used as a transport platform to the periphery for proteins such as cytoplasmic dynein and the dynactin complex for which a helical pfs arrangement is not disadvantageous. It is worth exploring the distinctive functions of unconventional MTs through the identification of MAPs that can recognize MTs with unconventional pfs numbers.
|
Academic Significance and Societal Importance of the Research Achievements |
非定型微小管の進化と神経機能の高度化の研究は高度な神経機能をもつヒトの中枢神経研究に多大な示唆を与えることが期待される。さらに非定型微小管が神経の部位によってどのような特殊性を持っているのかの解析、たとえば運動神経と感覚神経でどのように異なっているのかの解明はALSの病態生理の解明に資することが期待される。さらに中枢神経系の部位特異的なパターンの解析はその領域での軸索伸展やネットワーク形成の制御の解明につながることが期待される一方、その機構の破綻は神経変性疾患や精神神経疾患の病態解明や新たな治療戦略の創出につながることが期待される。
|
Report
(4 results)
Research Products
(4 results)
-
-
-
-
[Journal Article] Katanin p80, NuMA and cytoplasmic dynein cooperate to control microtubule dynamics2017
Author(s)
Mingyue Jin, Oz Pomp, Tomoyasu Shinoda, Shiori Toba, Takayuki Torisawa, Ken’ya Furuta, Kazuhiro Oiwa, Takuo Yasunaga, Daiju Kitagawa, Shigeru Matsumura, Takaki Miyata, Thong Teck Tan, Bruno Reversade & Shinji Hirotsune
-
Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 39902-39902
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research