|Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|Outline of Final Research Achievements
Ampullary carcinoma is rare and a highly malignant neoplasm. Therefore, the genomic biology of ampullary carcinomas is currently poorly defined. We have conducted the in-depth analysis of the genomic abnormalities of these carcinomas through an international multi-center collaboration. We identified a characteristic significantly mutated driver gene (ELF3) in these carcinomas by whole-exome sequencing. In this study, we performed transcriptome analysis using these samples and functional analysis of ELF3 by using an immortalized normal epithelial cell line of common bile duct origin. Ampullary carcinomas can be separated into two histological phenotype, intestinal-type or pancreatobilliary-type. The transcriptome analysis demonstrated the different clusters based on the histological phenotype. Functional studies demonstrated that ELF3 silencing in normal human epithelial cells enhances abnormalities of several pathways.