Three-dimensional human placental buds synthesized from induced pluripotent stem cells for regenerative therapy that can restore placental function
| Project/Area Number |
17H04339
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kyoto University |
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Principal Investigator |
Kondoh Eiji 京都大学, 医学研究科, 准教授 (10544950)
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| Co-Investigator(Kenkyū-buntansha) |
曽根 正勝 京都大学, 医学研究科, 特定准教授 (40437207)
山原 研一 兵庫医科大学, 医学部, 准教授 (50450888)
千草 義継 京都大学, 医学研究科, 助教 (80779158)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | placental organoid / 胎盤形成不全 / 胎盤機能再生医療 / 妊娠高血圧腎症 / 胎児機能不全 / iPS細胞 / 人工胎盤 / 胎盤機能不全 / 妊娠高血圧症候群 / ヒトiPS細胞 / 胎盤機能再生 / 器官芽 |
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| Outline of Final Research Achievements |
Human iPS cell-derived trophoblast-like cells, human amnion-derived mesenchymal stem cells, and human umbilical vein endothelial cells were co-cultured to produce a three-dimensional organ bud (mini placenta) that produces human chorionic gonadotropin (hCG). Immunohistochemistry confirmed that three types of trophoblasts coexist and localize to this mini-placenta. Therefore, the mini-placenta established in this study may be used as an in vitro 3D model that reflects trophoblast differentiation during placenta formation. This mini-placenta has also been confirmed to engraft immunodeficient mice and might be used as an in vivo model of placental formation.
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| Academic Significance and Societal Importance of the Research Achievements |
早発型の妊娠高血圧腎症（pre-eclampsia; PE）や胎児発育不全（fetal growth restriction; FGR）の児の予後は不良である。PE、FGRは胎盤形成不全に起因しており、胎盤機能再生治療の開発は喫緊の課題である。本研究で作成したミニ胎盤は、より生体に近い機能的な胎盤組織培養系としても使用でき胎盤を対象とした研究が飛躍的に進むことが期待される。また、ミニ胎盤を生着させたヒト化マウスは病的胎盤に起因する産科疾患のin vivoモデルとして、個別化医療に向けた研究材料としても有望であり、当該研究分野において革新的な研究手法の確立に貢献する可能性がある。
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Report
(4 results)
Research Products
(3 results)
