Development of multicontrast three-dimensional ocular imaging system using novel bioimaging techniques

• Project/Area Number: 17H04350
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: University of Tsukuba
• Principal Investigator: Oshika Tetsuro 筑波大学, 医学医療系, 教授 (90194133)
• Co-Investigator(Kenkyū-buntansha): 安野 嘉晃 筑波大学, 医学医療系, 教授 (10344871) ; 岡本 史樹 筑波大学, 医学医療系, 講師 (30334064) ; 平岡 孝浩 筑波大学, 医学医療系, 准教授 (30359575) ; 加治 優一 筑波大学, 医学医療系, 准教授 (50361332)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
• Keywords: 光干渉断層計 / OCT / 偏光感受型 / 複屈折 / phase retardation / 円錐角膜 / 濾過胞 / 線維化 / 偏光 / 緑内障 / 緑内障手術 / Jones matrix / イメージング

Outline of Final Research Achievements

We developed a novel multicontrast three-dimensional ocular imaging system using original bioimaging techniques. By analyzing the phase retardation of incident light signals into the eye, the device can conduct in vivo evaluation of collagen fiber degeneration and changes in tissue strength of the eye. The test model was engineered and registered as a designated management medical equipment (class II). New findings were obtained in human eyes with early keratoconus and after glaucoma filtering surgery.

Academic Significance and Societal Importance of the Research Achievements

眼の形状を検査する方法はこれまでに様々なものが開発されてきたが，眼の組織の質的変化や強度といった情報を，in vivoにおいて非侵襲で評価する方法はこれまでに無かった．多くの眼疾患は，組織のコラーゲンが変性して生じたり，強度が変化することが発症の引き金になる．眼に対する手術後の評価でもこういった情報は重要である．我々は新しい光干渉断層計を開発し，世界に先駆けてヒト眼の前眼部疾患に応用することにより，種々の知見を得ている．

Research Products

• [Journal Article] Volumetric multi-contrast dynamics imaging for ex vivo liver microvasculature activity visualization using Jones matrix optical coherence tomography (2021)
  • Author(s): Mukherjee P, Miyazawa A, Fukuda S, Yamashita T, Abd El-Sadek IG, Makita S, Oshika T, Yasuno Y
  • Journal Title: Proc. SPIE Volume: 11630 Pages: 11630-11630
  • Peer Reviewed
• [Journal Article] Optical coherence tomography-based tissue dynamics imaging for longitudinal and drug response evaluation of tumor spheroids (2020)
  • Author(s): Abd El-Sadek Ibrahim、Miyazawa Arata、Tzu-Wei Shen Larina、Makita Shuichi、Fukuda Shinichi、Yamashita Toshiharu、Oka Yuki、Mukherjee Pradipta、Matsusaka Satoshi、Oshika Tetsuro、Kano Hideaki、Yasuno Yoshiaki
  • Journal Title: Biomedical Optics Express Volume: 11 Issue: 11 Pages: 6231-6231
  • DOI: 10.1364/boe.404336
  • NAID: 120007192296
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of anterior segment and lens biometric measurements in patients with cataract (2020)
  • Author(s): Fukuda S, Ueno Y, Fujita A, Mori H, Tasaki K, Murakami T, Beheregaray S, Oshika T
  • Journal Title: Graefes Arch Clin Exp Ophthalmol Volume: 258 Issue: 1 Pages: 137-146
  • DOI: 10.1007/s00417-019-04482-0
  • Peer Reviewed
• [Journal Article] Comparison of intensity, phase retardation, and local birefringence images for filtering blebs using polarization-sensitive optical coherence tomography (2018)
  • Author(s): Fukuda S, Fujita A, Kasaragod D, Beheregaray S, Ueno Y, Yasuno Y, Oshika T
  • Journal Title: Sci Rep Volume: 8 Issue: 1 Pages: 7519-7519
  • DOI: 10.1038/s41598-018-25884-w
  • NAID: 120007134065
  • Peer Reviewed
• [Journal Article] Effects of astigmatic defocus on binocular contrast sensitivity (2018)
  • Author(s): Hasegawa Y, Hiraoka T, Nakano S, Okamoto F, Oshika T
  • Journal Title: PLoS One Volume: 13 Issue: 8 Pages: e0202340-e0202340
  • DOI: 10.1371/journal.pone.0202340
  • NAID: 120007128124
  • Peer Reviewed
• [Journal Article] SHG-specificity of cellular Rootletin filaments enables naïve imaging with universal conservation (2017)
  • Author(s): Toshihiro Akiyama, Akihito Inoko, Yuichi Kaji, Shigenobu Yonemura, Kisa Kakiguchi, Hiroki Segawa, Kei Ishitsuka, Masaki Yoshida, Osamu Numata, Philippe Leproux, Vincent Couderc, Tetsuro Oshika, Hideaki Kano
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 39967
  • DOI: 10.1038/srep39967
  • NAID: 120007135221
  • URL: https://tokushima-u.repo.nii.ac.jp/records/2005531
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Raman microscopy: A noninvasive method to visualize the localizations of biomolecules in the cornea (2017)
  • Author(s): Kaji Y, Akiyama T, Segawa H, Oshika T, Kano H
  • Journal Title: Cornea Volume: 36 Issue: 1 Pages: S67-S71
  • DOI: 10.1097/ico.0000000000001369
  • Peer Reviewed
• [Journal Article] Voltage-controlled IPMC actuators for accommodating intra-ocular lens systems (2017)
  • Author(s): Horiuchi T, Mihashi T, Fujikado T, Oshika T, Asaka K
  • Journal Title: Smart Materials and Structure Volume: 26
  • Peer Reviewed
• [Presentation] 偏光OCTで測定した角膜偏光位相差の加齢変化の検討 (2020)
  • Author(s): 上野勇太，森 悠大，福田慎一，大鹿哲郎
  • Organizer: 角膜カンファランス2020
• [Presentation] 偏光OCTを用いた角膜偏光位相差測定ソフトの繰り返し精度の検討 (2020)
  • Author(s): 森 悠大，上野勇太，福田慎一，大鹿哲郎
  • Organizer: 角膜カンファランス2020
• [Presentation] 光トポグラフィを用いた白内障手術前後の脳血流評価 (2020)
  • Author(s): 木住野源一郎，加治優一，菊地千一郎，小沢忠彦，高橋秀徳，井上裕治，大鹿哲郎，川島秀俊
  • Organizer: 第124回日本眼科学会総会
• [Presentation] 前眼部OCTを使用した蛋白質溶液および標準粒子溶液の定量評価 (2020)
  • Author(s): 上野勇太，高木星宇，大鹿哲郎
  • Organizer: 第124回日本眼科学会総会
• [Presentation] OCT with auto-alignment function: fundus shape model for myopia screening (2020)
  • Author(s): Palchunova K，三橋俊文，三野聡大，Liu J，平岡孝浩，長谷川優実，大鹿哲郎
  • Organizer: 第56回日本眼光学学会
• [Presentation] 高深達前眼部OCTによる前部硝子体剥離の観察 (2020)
  • Author(s): 福田慎一，森 悠大，上野勇太，藤田亜佳里，長谷川優実，平岡孝浩，大鹿哲郎
  • Organizer: 第74回日本臨床眼科学会
• [Presentation] 角膜厚を考慮した正常角膜・円錐角膜・円錐角膜僚眼の偏光位相差の比較 (2020)
  • Author(s): 藤田亜佳里，上野勇太，福田慎一，大鹿哲郎
  • Organizer: 第74回日本臨床眼科学会
• [Presentation] 3D label-free dynamics imaging of liver microvasculature by optical coherence microscopy (2020)
  • Author(s): Mukherjee P, Miyazawa A, Fukuda S, Yamashita T, Abd El-Sadek IG), Makita S, Oshika T, Yasuno Y
  • Organizer: Optics & Photonics Japan 2020
  • Int'l Joint Research
• [Presentation] 速広画角偏光OCTで撮影した正常眼と円錐角膜の偏光位相差の比較 (2019)
  • Author(s): 藤田亜佳里，上野勇太，阿部里沙，山成正宏，大鹿哲郎
  • Organizer: 第73回日本臨床眼科学会
• [Presentation] 高速広画角偏光OCTを使用した緑内障術後濾過胞の局所複屈折の評価 (2019)
  • Author(s): 藤井寛紀，上野勇太，藤田亜佳里，山成正宏，大鹿哲郎
  • Organizer: 第73回日本臨床眼科学会
• [Presentation] Birefringence dynamics imaging for ex-vivo tissue activity quantification using polarization-sensitive optical coherence tomography (2019)
  • Author(s): Mukherjee P, Abd El-Sadek IG, Miyazawa A, Shen LTW, Fukuda S, Yamashita T, Oka Y, Matsusaka S, Oshika T, Kano H, Yasuno Y
  • Organizer: Optics & Photonics Japan 2019
  • Invited
• [Presentation] Short and long time-course ex vivo tissues activity quantification by multifunctional OCT (2019)
  • Author(s): Abd El-Sadek IG, Miyazawa A, Shen LTW, Fukuda S, Yamashita T, Oka Y, Mukherjee P, Makita S, Matsusaka S, Oshika T, Kano H, Yasuno Y
  • Organizer: Optics & Photonics Japan 2019
  • Invited
• [Presentation] Simultaneous imaging of scleral birefringence and biomechanics by Jones matrix optical coherence elastography (2018)
  • Author(s): Li E, Ueno Y, Makita S, Oshika T, Yasuno Y
  • Organizer: 2018 ARVO Imaging in the Eye Conference
  • Int'l Joint Research
• [Presentation] An attempt to determine normal range of corneal higher-order aberrations (2018)
  • Author(s): Hiraoka T, Kiuchi G, Ueno U, Oshika T
  • Organizer: Association for Research in Vision and Ophthalmology
  • Int'l Joint Research
• [Presentation] Transparency distribution of cataractous crystalline lens from analysis of images obtained by a Shack-Hartmann wavefront aberrometer (2018)
  • Author(s): Mihashi T, Hirohara Y, Morishima S, Mori H, Hiraoka T, Okamoto F, Oshika T
  • Organizer: Association for Research in Vision and Ophthalmology
  • Int'l Joint Research
• [Presentation] CASIA2を使用した嚢内固定されている眼内レンズ度数の推測 (2018)
  • Author(s): 上野勇太，藤田亜佳里，高橋尚子，阿部里沙，大鹿哲郎
  • Organizer: 第72回日本眼科臨床眼科学会
• [Presentation] CASIA2を使用した3種類の眼内レンズ形態の比較 (2018)
  • Author(s): 藤田亜佳里，上野勇太，高橋尚子，阿部里沙，大鹿哲郎
  • Organizer: 第72回日本眼科臨床眼科学会
• [Presentation] Evaluation of filtration surgery outcome using multi contrast anterior eye segment Jones matrix optical coherence tomography (2017)
  • Author(s): Kasaragod D, Makita S, Ueno Y, Hoshi S, Fukuda S, Oshika T, Yasuno Y:
  • Organizer: BIOS 2017 - Ophthalmic Technologies XXVII
  • Int'l Joint Research
• [Presentation] Birefringent imaging of limbal region using Jones matrix optical coherence tomography (2017)
  • Author(s): Kasaragod D, Fujita A, Makita S, Ueno Y, Hoshi S, Okubo T, Oshika T, Yasuno Y
  • Organizer: Association for Research in Vision and Ophthalmology 2017
  • Int'l Joint Research
• [Presentation] Visualization of depth-localized birefringence of pterygium using polarization sensitive optical coherence tomography (2017)
  • Author(s): Hoshi S, Kasaragod D, Ueno Y, Fujita A, Fukuda S, Yasuno Y, Oshika T
  • Organizer: Association for Research in Vision and Ophthalmology 2017
  • Int'l Joint Research
• [Presentation] Evaluation of fibrosis in filtering bleb before and after needling revision using polarization-sensitive optical coherence tomography: two case reports (2017)
  • Author(s): Tasaki K, Ueno Y, Kasaragod D, Hoshi S, Fujita A, Okubo T, Fukuda S, Yasuno Y, Oshika T
  • Organizer: Association for Research in Vision and Ophthalmology 2017
  • Int'l Joint Research
• [Presentation] Longitudinal changes in the fibrosis score of filtering bleb using polarization-sensitive optical coherence tomography (2017)
  • Author(s): Ueno T, Kasaragod K, Hoshi S, Fujita A, Okubo T, Fukuda S, Yasuno Y, Oshika T
  • Organizer: Association for Research in Vision and Ophthalmology 2017