| Project/Area Number |
17H04399
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
窪木 拓男 岡山大学, 医歯薬学総合研究科, 教授 (00225195)
大野 充昭 岡山大学, 医歯薬学総合研究科, 准教授 (60613156)
佐藤 守俊 東京大学, 大学院総合文化研究科, 教授 (00323501)
戸村 道夫 大阪大谷大学, 薬学部, 教授 (30314321)
戸口田 淳也 京都大学, ウイルス・再生医科学研究所, 教授 (40273502)
渡辺 亮 京都大学, iPS細胞研究所, 特定拠点助教 (60506765)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | 光操作 / 遺伝子改変マウス / 間葉系幹細胞 / 光操作技術 / 幹細胞 |
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| Outline of Final Research Achievements |
In this study, we generated a tTA-dependent photoactivatable Cre-loxP recombinase knock-in mouse model (TRE-PA-Cre mice) using a CRISPR/Cas9 system. These mice were crossed with ROSA26-tdTomato mice (Cre reporter mouse) to visualize DNA recombination as marked by tdTomato expression. We demonstrated that external noninvasive LED blue light illumination allows efficient DNA recombination in the liver of TRE-PA-Cre:ROSA26-tdTomato mice transfected with tTA expression vectors using hydrodynamic tail vein injection. The TRE-PA-Cre mouse established here promises to be useful for optogenetic genome engineering in a noninvasive, spatiotemporal, and cell-type specific manner in vivo.
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| Academic Significance and Societal Importance of the Research Achievements |
従来不可能であった「時間・空間（光照射時/部位）特異的」な精度を持つ生体内遺伝子操作が可能となり、今後、様々な組織・細胞で生物医学研究の新規戦略手法として幅広く使用されることが期待される。
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