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Natural history of rain frogs: elucidating the adaptive evolution of frog glue

Research Project

Project/Area Number 17H04608
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Biodiversity/Systematics
Research InstitutionNagahama Institute of Bio-Science and Technology (2018-2021)
Hiroshima University (2017)

Principal Investigator

Atsushi Kurabayashi  長浜バイオ大学, バイオサイエンス学部, 准教授 (00327701)

Co-Investigator(Kenkyū-buntansha) 熊澤 慶伯  名古屋市立大学, 大学院理学研究科, 教授 (60221941)
森 哲  京都大学, 理学研究科, 教授 (80271005)
土岐田 昌和  東邦大学, 理学部, 准教授 (80422921)
澤田 均  名古屋大学, 理学研究科, 教授 (60158946)
Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2020: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords生体糊 / アフリカ / フクラガエル / 形質進化 / 環境要因 / 接着物質 / mRNA-Seq / 相関分析 / 適応進化 / プロテオーム / 南アフリカ / アミノ酸組成 / RNA-Seq / 新産地 / フクラガエル属 / 有用生体物質 / 繁殖生態 / タンパク質 / フクラガエル科 / 正の淘汰 / 生体機能分子 / 機能進化 / 遺伝子進化 / 比較系統 / 自然史 / フクラガエル類 / プロテオミクス / 糊状物質 / プロテオーム解析 / mRNA解析
Outline of Final Research Achievements

The genus Breviceps is known as a strange tetrapod in which males and females mate by gluing their bodies together using “glue secretion” from their skin. We have studied the glue secretion, which have remained a mystery for 60 years, and have gained new knowledge on their physical characteristics such as adhesive strength and duration, chemical composition such as water and amino acid contents, and glue proteins and their candidate genes. Furthermore, fieldwork was conducted in South Africa, where the adhesive strength of glue was measured in 13 Breviceps species, and differences in glue adhesion between these species were found. Furthermore, which morphological and ecological factors are responsible for the strength of glue adhesion between species was examined.

Academic Significance and Societal Importance of the Research Achievements

生殖時に雌雄の接着に用いられるフクラガエル糊は、そのユニークさから世界的に注目されていたが、任意のタイミングで採取ができなかったため、60年間研究が進んでいなかった。本研究では、電気刺激により任意に糊粘液を分泌させる技術を応用し、さらに、フィールドワークを組み合わせた研究を遂行することで、フクラガエル糊について多くの知見を得た。これは動物学上の長年の謎に答えを与えると同時に、カエル糊の工業利用を行う上で必要な基礎データでもある。なお、成果の一部を論文として公表したが、これは新聞4社で紹介され、社会的反響も大きかった。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (9 results)

All 2021 2020 2017 Other

All Int'l Joint Research (4 results) Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (1 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Int'l Joint Research] ノースウェスト大学(南アフリカ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] ノースウェスト大学(南アフリカ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] North West University(南アフリカ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ノースウェスト大学(南アフリカ)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Better than mere attraction - adhesive properties of skin secretion in the Common Rain Frog, Breviceps adspersus2021

    • Author(s)
      Kakehashi, R., Hemmi, K., Landman, W., Furuno, N., Du Preez, L., Minter. L., and Kurabayashi, A.
    • Journal Title

      Salamandra

      Volume: 58 Pages: 43-51

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Exceptional Enlargement of the Mitochondrial Genome Results from Distinct Causes in Different Rain Frogs (Anura: Brevicipitidae: Breviceps)2020

    • Author(s)
      Hemmi Keitaro、Kakehashi Ryosuke、Kambayashi Chiaki、Du Preez Louis、Minter Leslie、Furuno Nobuaki、Kurabayashi Atsushi
    • Journal Title

      International Journal of Genomics

      Volume: 2020 Pages: 1-12

    • DOI

      10.1155/2020/6540343

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Attempt at artificial breeding and analysis of mitochondrial genomes in Breviceps frogs.2017

    • Author(s)
      Hemmi, K., R. Kakehashi, L.D. Preez, L. Minter, A. Kurabayashi
    • Organizer
      The 8th International Conference on Global Resource Conservation
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 進化・多様性生物学 研究室(長浜バイオ大学)

    • URL

      https://biodiv-evolbiol-lab.jimdosite.com/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 夫婦の絆はどれだけ強い?フクラガエルの謎を解く

    • URL

      https://www.nagahama-i-bio.ac.jp/research/

    • Related Report
      2021 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2023-01-30  

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