|Budget Amount *help
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2017: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
|Outline of Final Research Achievements
The effect of muscle-derived exosomes on innate immunity was investigated by the experimental system treating bone marrow-derived macrophages with the supernatant of cultured myotubes or extracted exosomes from the media.
M1 polarity (pro-inflammatory) of macrophages was downregulated by the addition of myotube supernatant or muscle exosomes. Ultrasound irradiation to myotubes doubled the release of muscle exosomes, and the ultrasound stimulation of the myotube enhanced the inhibitory effect of myotube supernatant on M1 polarity of macrophages. In addition, the effect of ultrasound was canceled by depleting exosomes from the culture supernatant.
This study suggested that the anti-inflammatory effect of skeletal muscle was exerted via exosomes.