Revelation of the neuronal mechanism of motor learning in non-human primates with the two-photon calcium imaging
Project/Area Number |
17H04982
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | Institute of Physical and Chemical Research (2019-2020) The University of Tokyo (2017-2018) |
Principal Investigator |
Masamizu Yoshito 国立研究開発法人理化学研究所, 脳神経科学研究センター, 副チームリーダー (90608530)
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Project Period (FY) |
2017-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2017: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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Keywords | in vivo カルシウムイメージング / 運動学習 / マーモセット / カルシウムイメージング / 2光子カルシウムイメージング / 2光子カルシウムイメージング |
Outline of Final Research Achievements |
Two-photon calcium imaging has revealed neuronal activities related to behavioral and cognitive functions in rodents at a single-cell resolution. To apply this technique to behaving non-human primates, we developed protocols to train head-fixed common marmosets to perform upper-limb movement tasks. Head-fixed marmosets could control a pole with an upper limb to move a cursor to a target on a screen. We conducted two-photon calcium imaging of layer 2/3 neurons in the motor cortex during this motor task performance, and detected task-relevant activity from neuronal somata, dendrites and axons.
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Academic Significance and Societal Importance of the Research Achievements |
我々は、霊長類コモンマーモセットで、課題実行時に脳のin vivo 2光子カルシウムイメージングをおこなう系を確立した。今後、この系を用いて、様々な精神・神経疾患モデルの遺伝子改変コモンマーモセットの神経基盤を解明することによって、疾患における神経ネットワーク変容の理解に役立てることで、新たな治療方法の開発につながることが期待できる。
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Report
(5 results)
Research Products
(7 results)
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[Journal Article] Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks2018
Author(s)
Ebina T, Masamizu Y, Tanaka YR, Watakabe A, Hirakawa R, Hirayama Y, Hira R, Terada S, Koketsu D, Hikosaka K, Mizukami H, Nambu A, Sasaki E, Yamamori Y, Matsuzaki M
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Journal Title
Nat Commun
Volume: 9
Issue: 1
Pages: 1879-1879
DOI
Related Report
Peer Reviewed / Open Access
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