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Revelation of the neuronal mechanism of motor learning in non-human primates with the two-photon calcium imaging

Research Project

Project/Area Number 17H04982
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Neurophysiology / General neuroscience
Research InstitutionInstitute of Physical and Chemical Research (2019-2020)
The University of Tokyo (2017-2018)

Principal Investigator

Masamizu Yoshito  国立研究開発法人理化学研究所, 脳神経科学研究センター, 副チームリーダー (90608530)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2017: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywordsin vivo カルシウムイメージング / 運動学習 / マーモセット / カルシウムイメージング / 2光子カルシウムイメージング / 2光子カルシウムイメージング
Outline of Final Research Achievements

Two-photon calcium imaging has revealed neuronal activities related to behavioral and cognitive functions in rodents at a single-cell resolution. To apply this technique to behaving non-human primates, we developed protocols to train head-fixed common marmosets to perform upper-limb movement tasks. Head-fixed marmosets could control a pole with an upper limb to move a cursor to a target on a screen. We conducted two-photon calcium imaging of layer 2/3 neurons in the motor cortex during this motor task performance, and detected task-relevant activity from neuronal somata, dendrites and axons.

Academic Significance and Societal Importance of the Research Achievements

我々は、霊長類コモンマーモセットで、課題実行時に脳のin vivo 2光子カルシウムイメージングをおこなう系を確立した。今後、この系を用いて、様々な精神・神経疾患モデルの遺伝子改変コモンマーモセットの神経基盤を解明することによって、疾患における神経ネットワーク変容の理解に役立てることで、新たな治療方法の開発につながることが期待できる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (7 results)

All 2020 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (2 results)

  • [Journal Article] Arm movements induced by noninvasive optogenetic stimulation of the motor cortex in the common marmoset2019

    • Author(s)
      Ebina Teppei、Obara Keitaro、Watakabe Akiya、Masamizu Yoshito、Terada Shin-Ichiro、Matoba Ryota、Takaji Masafumi、Hatanaka Nobuhiko、Nambu Atsushi、Mizukami Hiroaki、Yamamori Tetsuo、Matsuzaki Masanori
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 116 Issue: 45 Pages: 22844-22850

    • DOI

      10.1073/pnas.1903445116

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks2018

    • Author(s)
      Ebina T, Masamizu Y, Tanaka YR, Watakabe A, Hirakawa R, Hirayama Y, Hira R, Terada S, Koketsu D, Hikosaka K, Mizukami H, Nambu A, Sasaki E, Yamamori Y, Matsuzaki M
    • Journal Title

      Nat Commun

      Volume: 9 Issue: 1 Pages: 1879-1879

    • DOI

      10.1038/s41467-018-04286-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Two-photon calcium imaging in the motor cortex of non-human primates during movement task2020

    • Author(s)
      Yoshito Masamizu
    • Organizer
      The 9th RIEC International Symposium on Brain Functions and Brain Computer
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 構成的アプローチによって脳の情報処理機構を解明するための基盤技術開発2019

    • Author(s)
      正水 芳人
    • Organizer
      行動の多様性を支える神経基盤とその動作様式の解明
    • Related Report
      2019 Annual Research Report
  • [Presentation] Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks.2018

    • Author(s)
      正水 芳人
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Book] ブレインサイエンス・レビュー 20202020

    • Author(s)
      正水 芳人(担当:分担執筆, 範囲:霊長類脳のin vivo 2光子カルシウムイメージング)
    • Total Pages
      20
    • Publisher
      クバプロ
    • ISBN
      9784878051647
    • Related Report
      2019 Annual Research Report
  • [Book] マーモセットラボマニュアル、6.2 2光子顕微鏡を用いた脳のin vivoカルシウムイメージング2018

    • Author(s)
      正水 芳人、松崎 政紀
    • Total Pages
      6
    • Publisher
      アドスリー
    • ISBN
      9784904419786
    • Related Report
      2018 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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