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Unbiased analysis and therapeutic strategies on cancer cell dormancy by using optical imaging techniques

Research Project

Project/Area Number 17H04989
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Tumor biology
Research InstitutionJichi Medical University

Principal Investigator

Kuchimaru Takahiro  自治医科大学, 医学部, 講師 (10570591)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Keywords転移 / 細胞間相互作用 / 近接細胞蛍光標識技術 / 一細胞オミクス解析 / 光標識技術 / 光イメージング / 単一細胞マルチオミクス解析 / 骨転移 / ニッチ / 冬眠がん細胞 / 単一細胞解析 / 冬眠
Outline of Final Research Achievements

Most of death from cancer is caused by metastasis. Cancer-stroma interactions are supposed to play critical roles to establish metastasis. To identify and characterize stromal cells interacting with cancer cells in murine metastasis models, we developed a genetically encoded fluorescent labeling technology named sGRAPHIC. sGRAPHIC enables efficient fluorescent labeling of proximal stromal cells interacting with cancer cells in living tissues. sGRAPHIC-labeled stomal cells can be harvested by a fluorescent activated cell sorter. Single cell omics analysis would be powerful to characterize the isolated stromal cells by sGRAPHIC, elucidating cancer-stroma interactions in metastasis.

Academic Significance and Societal Importance of the Research Achievements

がん細胞と組織間質細胞の相互作用の解析は、これまで組織切片試料の観察から得られるごく限られた情報から構築された仮説に基づいて進められてきた。本研究で開発したsGRAPHICによって、がん細胞と相互作用する組織間質細胞のオミクス解析が実現すれば、相互作用に関する包括的な情報が手に入り、転移の成立を制御する決定的な分子機構が明らかになる可能性が高い。それらは、転移の予防法の確立など、がんを撲滅する新しいアプローチの礎となるはずである。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (6 results)

All 2019

All Presentation (6 results) (of which Invited: 1 results)

  • [Presentation] A novel technology for fluorescent labeling of cells involved in cellular interactions in living tissues2019

    • Author(s)
      峯岸美紗、近藤科江、口丸高弘
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] がん細胞-間質細胞相互作用解析のための新規蛍光イメージング技術2019

    • Author(s)
      口丸高弘
    • Organizer
      第7回細胞凝集研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] がん細胞-間質細胞相互作用解析のための近接細胞蛍光標識技術2019

    • Author(s)
      口丸高弘、峯岸美紗、近藤科江
    • Organizer
      第17回がんとハイポキシア研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] A novel technology for optically labeling stromal cells interacting with cancer cells in the metastatic microenvironment,2019

    • Author(s)
      Misa Minegishi, Shinae Kondoh, Takahiro Kuchimaru
    • Organizer
      第78回日本癌学会学術総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細胞間相互作用のシングルセル解析を指向した蛍光タンパク質技術2019

    • Author(s)
      口丸高弘
    • Organizer
      シングルセルゲノミクス研究会2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] 生体組織で転移ニッチ構成細胞を蛍光標識する新規技術の開発2019

    • Author(s)
      峯岸美紗、宮内ひとみ、近藤科江、口丸高弘
    • Organizer
      第28回日本がん転移学会学術集会・総会
    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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