Creation of artificial enzymes that enable targeted editing of any DNA bases
Project/Area Number |
17H04994
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
System genome science
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Research Institution | Kobe University |
Principal Investigator |
Nishida Keiji 神戸大学, 先端バイオ工学研究センター, 教授 (10620338)
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Project Period (FY) |
2017-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2020: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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Keywords | ゲノム編集 / CRISPR / 塩基編集 / 合成生物学 / Target-AID / Base editing / synthetic biology / 合成生物 / バイオテクノロジー / ゲノム / 遺伝子 |
Outline of Final Research Achievements |
As a new base-editing technology, we have succeeded in creating an artificial enzyme that can be expressed in cells without causing genomic instability using DNA glycosylase as a matrix, and can debase thymine only in targeted regions. This enabled the conversion of thymine to other bases. Furthermore, they increased the efficiency by adding factors involved in post-debase repair. In order to increase the versatility of the enzyme, they were able to find a way to make it work in combination with CRISPR variants.
Translated with www.DeepL.com/Translator (free version)
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Academic Significance and Societal Importance of the Research Achievements |
生物のゲノムを書き換えることができる新たなゲノム編集技術として、これまで困難であったTの一文字の書き換えに関わる酵素について、自然界では異なる文字を認識する酵素を改変することによって新たに生み出すことに成功した。これによってより自由度の高いゲノム情報の書き換えが可能になり、遺伝子治療法や、微生物・農水産物の育種技術として応用可能性が期待される。
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Report
(5 results)
Research Products
(19 results)
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[Journal Article] Base editors for simultaneous introduction of C-to-T and A-to-G mutations2020
Author(s)
Sakata Rina C.、Ishiguro Soh、Mori Hideto、Tanaka Mamoru、Tatsuno Kenji、Ueda Hiroki、Yamamoto Shogo、Seki Motoaki、Masuyama Nanami、Nishida Keiji、Nishimasu Hiroshi、Arakawa Kazuharu、Kondo Akihiko、Nureki Osamu、Tomita Masaru、Aburatani Hiroyuki、Yachie Nozomu
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Journal Title
Nature Biotechnology
Volume: na
Issue: 7
Pages: 865-869
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] In vivo targeted single-nucleotide editing in zebrafish.2018
Author(s)
Tanaka, S., Yoshioka, S., Nishida, K., Hosokawa, H., Kakizuka, A., and Maegawa, S.
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Journal Title
Sci. Rep.
Volume: 8
Issue: 1
Pages: 11423-11423
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments2017
Author(s)
Yoshida Yamato, Kuroiwa Haruko, Shimada Takashi, Yoshida Masaki, Ohnuma Mio, Fujiwara Takayuki, Imoto Yuuta, Yagisawa Fumi, Nishida Keiji, Hirooka Shunsuke, Misumi Osami, Mogi Yuko, Akakabe Yoshihiko, Matsushita Kazunobu, Kuroiwa Tsuneyoshi
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 114
Issue: 50
Pages: 13284-13289
DOI
Related Report
Peer Reviewed / Open Access
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