|Budget Amount *help
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
|Outline of Final Research Achievements
Although EMT has been studied in vivo and in the developmental context, aberrant EMT, which mimics certain human pathogenesis, has not been systematically investigated due to the lack of tractable in vivo model.To characterize the initial cellular events following spindle misorientaiton, I took a live imaging approach to monitor the localization of junction and polarity proteins and examine the cytoskeletal dynamics during misoriented cell division and the following basal movement. In order to characterize the molecular signatures of EMT effects, I performed genome-wide transcriptional profiling by isolating mesenchymal-like cells via FACS and performing RNA sequencing analyses in both epithelial and mesenchymal-like cells. By defining the cellular and molecular features of spindle misorientation-induced EMT, this project has established a general mechanistic understanding of EMT events in proliferating epithelia.