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Search for germline epigenetic information that induces post-fertilization imprinted methylation

Research Project

Project/Area Number 17H05012
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Genetics/Chromosome dynamics
Research InstitutionUniversity of Tsukuba

Principal Investigator

Matsuzaki Hitomi  筑波大学, 生命環境系, 助教 (50436242)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Keywordsエピジェネティクス / ゲノム刷り込み / DNAメチル化 / 遺伝子 / 発現制御
Outline of Final Research Achievements

In mammals, mono-allelic expression of a subset of genes (genomic imprinting) is essential for normal development, which is governed by allele-specific DNA methylation at the imprinting control regions (ICRs) within the loci. In the present study, I searched for epigenetic information used to discriminate parental alleles in the process of post-fertilization imprinted DNA methylation of the ICR at the Igf2/H19 locus. In addition, I identified cis sequences which are necessary and sufficient for the regulation.

Academic Significance and Societal Importance of the Research Achievements

ヒトでは、刷り込み遺伝子のDNAメチル化異常による疾患が知られている。また、近年、親が曝された環境要因によりエピジェネティックな変化が配偶子で生じ、子での遺伝子発現に影響を与える可能性も指摘されている。本研究による、受精後のアリル特異的DNAメチル化制御メカニズムの解析結果は、疾患の原因究明やエピジェネティック遺伝の実体の解明に向けて、基礎となる情報を提供できると期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (12 results)

All 2020 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (10 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Recapitulation of gametic DNA methylation and its post-fertilization maintenance with reassembled DNA elements at the mouse Igf2/H19 locus2020

    • Author(s)
      Matsuzaki, H., Kuramochi, D., Okamura, E., Hirakawa, K., Ushiki, A., and Tanimoto, K.
    • Journal Title

      Epigenetics & Chromatin

      Volume: 13 Issue: 1 Pages: 2-2

    • DOI

      10.1186/s13072-019-0326-1

    • NAID

      120007132843

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synthetic DNA fragments bearing ICR cis elements become differentially methylated and recapitulate genomic imprinting in transgenic mice2018

    • Author(s)
      Matsuzaki, H,. Okamura, E., Kuramochi, D., Ushiki, A., Hirakawa, K., Fukamizu, A., and Tanimoto, K.
    • Journal Title

      Epigenetics & Chromatin

      Volume: 11 Issue: 1 Pages: 36-36

    • DOI

      10.1186/s13072-018-0207-z

    • NAID

      120006939623

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Reconstituted H19 ICR sequence with identified cis elements is capable of maintaining genomic imprinting in knock-in mouse2019

    • Author(s)
      Matsuzaki, H., Kuramochi, D., Hirakawa, K., and Tanimoto, K.
    • Organizer
      33rd International Mammalian Genome Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Test for imprinted DNA methylation activity of the human H19-ICR in YAC transgenic mice by adopting a transgene co-placement strategy2019

    • Author(s)
      Hirakawa, K., Matsuzaki, H., and Tanimoto, K
    • Organizer
      The 15th Transgenic Technology Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウスのゲノム刷り込み制御におけるH19-ICR配列反転の効果2019

    • Author(s)
      宮嶋優、谷本啓司、松崎仁美
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] YACトランスジェニック・マウスを用いたヒトH19-ICR刷り込みメチル化メカニズムの検証2019

    • Author(s)
      平川勝彦、松崎仁美、谷本啓司
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] In vivo analysis of H19 gene transcriptional mechanism by inversion of the H19 imprinting control region2018

    • Author(s)
      Matsuzaki, H., Miyajima, Y., and Tanimoto, K
    • Organizer
      32nd International Mammalian Genome Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] H19-ICR反転によるH19遺伝子転写制御機構のin vivo解析2018

    • Author(s)
      宮嶋優、谷本啓司、松崎仁美
    • Organizer
      第12回日本エピジェネティクス研究会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ヒトβグロビン遺伝子座におけるH19-ICR断片挿入の効果とクロマチン立体構造解析2018

    • Author(s)
      平川勝彦、松﨑仁美、谷本啓司
    • Organizer
      第12回日本エピジェネティクス研究会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] H19-ICR 反転マウスを用いたゲノム刷り込み制御機構の解析2018

    • Author(s)
      松﨑仁美、宮嶋優、谷本啓司
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Imprinted gene expression can be reconstituted by synthetic DNA fragment composed of specified cis regulatory elements from the H19 ICR2018

    • Author(s)
      Matsuzaki, H., Kuramochi, D., Hirakawa, K., and Tanimoto, K.
    • Organizer
      Keystone Symposia (Gene Control in Development and Disease)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 人工刷り込み制御配列によるゲノム刷り込みの再構築2017

    • Author(s)
      倉持大地、松崎仁美、牛木亜季、谷本啓司
    • Organizer
      第64回日本実験動物学会総会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2021-12-27  

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