• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Why noroviruses are accumulated in oysters: To unravel the mechanisms

Research Project

Project/Area Number 17H06213
Research Category

Grant-in-Aid for Challenging Research (Pioneering)

Allocation TypeSingle-year Grants
Research Field Civil engineering and related fields
Research InstitutionTohoku University

Principal Investigator

Omura Tatsuo  東北大学, 未来科学技術共同研究センター, 名誉教授 (30111248)

Co-Investigator(Kenkyū-buntansha) 藤井 学  東京工業大学, 環境・社会理工学院, 特任准教授 (30598503)
三浦 尚之  国立保健医療科学院, その他部局等, 主任研究官 (70770014)
渡辺 幸三  愛媛大学, 理工学研究科(工学系), 教授 (80634435)
佐野 大輔  東北大学, 工学研究科, 准教授 (80550368)
Project Period (FY) 2017-06-30 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2019: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2018: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Keywordsノロウイルス / カキ / プランクトン / 遺伝子解析 / 生物濃縮 / 動物プランクトン / 植物プランクトン
Outline of Final Research Achievements

The dynamics of norovirus, one of the causative agent of infectious gastroenteritis, in the estuarine environment is not well understood. In this study, based on the hypothesis that planktons in seawater can be the important carrier of norovirus to oysters, field study was conducted. Norovirus GII RNA were detected from sorted zooplankton and phytoplankton samples, and oyster digestive tissues. The maximum concentrations of norovirus GII RNA were 3900 copies/L in plankton samples, 58 copies/individual of zooplankton, and 2000 copies/g of oyster digestive tissues. DNA metabarcoding analysis indicated that oysters prey on zooplankton (phylums Cercozoa and Ciliophora) and phytoplankton (phylum Chlorophyta, and Ranthkea_octopunctata). Correlation of norovirus GII with some specific planktonic species (e.g., Rathkea_octopunctata, Sarsia_tubulosa) were found, implying the important roles of such species as potential norovirus carrier.

Academic Significance and Societal Importance of the Research Achievements

年間一千万人を超える感染性胃腸炎患者数を減少させるには、これまでのワクチンの開発による予防医学的な手法だけでは不可能である。本申請プロジェクトにおけるノロウイルスのカキへの蓄積メカニズムの解明を突破口として、様々な病原ウイルスの自然環境や社会環境での動態を把握し、社会的な取り組みによって感染性胃腸炎流行のトリガーとなる食品や水の汚染を極力防止することが現状を打破するための非常に有効な手段と考える。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (31 results)

All 2019 2018

All Journal Article (6 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 6 results,  Open Access: 6 results) Presentation (22 results) (of which Int'l Joint Research: 14 results,  Invited: 5 results) Book (3 results)

  • [Journal Article] Unraveling the Genetic Structure of the Coconut Scale Insect Pest (Aspidiotus rigidus Reyne) Outbreak Populations in the Philippines2019

    • Author(s)
      Serrana, J. M., N. Ishitani, T. M. Carvajal, B. J. M. Almarinez, A. T. Barrion, D. M. Amalin, K. Watanabe
    • Journal Title

      Insects

      Volume: 10 (11) Issue: 11 Pages: 374-374

    • DOI

      10.3390/insects10110374

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of Wolbachia Infection in Field-collected Mosquito Vector, Aedes aegypti in Metropolitan Manila, Philippines2019

    • Author(s)
      Carvajal,T. M., K. Hashimoto, R. K. Harnandika, D. M. Amalin and K. Watanabe
    • Journal Title

      Parasites & Vectors

      Volume: 12 (1) Issue: 1 Pages: 361-361

    • DOI

      10.1186/s13071-019-3629-y

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparison of DNA Metabarcoding and Morphological Identification for Stream Macroinvertebrate Biodiversity Assessment and Monitoring2019

    • Author(s)
      Serrana, J. M., Y. Miyake, M. Gamboa and K. Watanabe
    • Journal Title

      Ecological Indicators

      Volume: 101 Pages: 963-972

    • DOI

      10.1016/j.ecolind.2019.02.008

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Weekly Variation of Rotavirus A Concentrations in Sewage and Oysters in Japan, 2014-20162019

    • Author(s)
      Ito, E., J. Pu, T. Miura, S. Kazama, M. Nishiyama, H. Ito, Y. Konta, G. T. Nguyen, T. Omura, T. Watanabe
    • Journal Title

      Pathogens

      Volume: 8 Issue: 3 Pages: 89-89

    • DOI

      10.3390/pathogens8030089

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Genetic Determinant of Norovirus for Susceptibility to Free Chlorine2018

    • Author(s)
      Andri Rachmadi, Masaaki Kitajima, Kozo Watanabe, Sakiko Yaegashi, Joeselle Serrana, Arata Nakamura, Toyoko Nakagomi, Osamu Nakagomi, Kazuhiko Kazuhiko Katayama, Satoshi Okabe, and Daisuke Sano
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 印刷中 Issue: 13

    • DOI

      10.1128/aem.00244-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ecological Influence of Sediment Bypass Tunnels on Macroinvertebrates in Dam-Fragmented Rivers by DNA Metabarcoding2018

    • Author(s)
      J. Serrana, S. Yaegashi, S. Kondoh, B. Li, C.T. Robinson, K. Watanabe
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 10185-10185

    • DOI

      10.1038/s41598-018-28624-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] "Development and assessment of Mozzify app: an integrated mhealth for real-time Dengue reporting and mapping, health communication and behavior modification reporting and mapping, health communication and behavior modification"2019

    • Author(s)
      Herbuela, Von Ralph Dane M., Karita Tomonori, Francisco, Micanaldo, Watanabe Kozo
    • Organizer
      1st JSPS Core-to-Core Symposium: Center of Excellence in Health Risk Assessment for Adaptation to Climate Change
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular Approaches to the Study of Freshwater Biodiversity: Genomic Adaptation and DNA Metabarcoding.2019

    • Author(s)
      Kozo Watanabe
    • Organizer
      International Workshop of Water Quality Monitoring
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Detection of Wolbachia in Field-collected Dengue Vector Mosquito, Aedes aegypti in the Philippines2019

    • Author(s)
      Kozo Watanabe
    • Organizer
      Philippine Society for Microbiology 48th Annual Convention
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] "Sediment Microbial Diversity and Community Composition of Restored Gravel Bars in the Trinity River, California "2019

    • Author(s)
      Joeselle Serrana, Bin Li, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe
    • Organizer
      Philippine Society for Microbiology 48th Annual Convention
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Contributing Factors to Childhood Stunting in Indonesia: Water Quality and Diarrhea2019

    • Author(s)
      Savira Ekawardhani, Hesti Lina Wiraswati, Lia Faridah, Kozo Watanabe
    • Organizer
      Water and Environment Technology Conference 2019 (WET2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparison of DNA metabarcoding and morphological identification for stream macroinvertebrate biodiversity assessment and monitoring2019

    • Author(s)
      Joeselle Serrana, Yo Miyake, Maribet Gamboa, Kozo Watanabe
    • Organizer
      Pest Management Council of the Philippines 51st Anniversary and Annual Scientific Conference
    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparative Performance of Formularity and Two Automated Bath Algorithms for Formula Assignment of Organic Matter with Other Methods2019

    • Author(s)
      Fu, Q-L., Fujii, M.
    • Organizer
      Water and Environment Technology Conference 2019 (WET2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 沿岸域における鉄と有機物の動態に関する研究2019

    • Author(s)
      藤井学, 吉村千洋
    • Organizer
      第22 回日本水環境学会シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Two-year monitoring of norovirus and rotavirus present in suspended and dissolved forms in drinking water sources in Japan2019

    • Author(s)
      Takayuki Miura, Arisa Gima, Marina Tokuyasu, Michihiro Akiba
    • Organizer
      20th Symposium on Health-Related Water Microbiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 水環境におけるノロウイルスの挙動と養殖カキを原因とする食中毒対策2019

    • Author(s)
      三浦尚之
    • Organizer
      農林水産省令和元年度食品安全に係る科学セミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Sediment Microbial Diversity and Community Composition of Restored Gravel Bars in the Trinity River, California2019

    • Author(s)
      Joeselle Serrana, Bin Li, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe
    • Organizer
      ,American Society for Microbiology (ASM) Microbe
    • Related Report
      2018 Annual Research Report
  • [Presentation] 病原ウイルスの表流水中存在形態を考慮した 汚染指標に関する検討2019

    • Author(s)
      三浦尚之,鈴木知美,儀間ありさ,越後信哉,秋葉道宏
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 水性藍藻類の細胞酸化ストレスならびに毒素生成に及ぼす水質因子の影響2019

    • Author(s)
      那須川康平, 藤井学
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ノロウイルスの水環境中動態:ヒトからカキへ2018

    • Author(s)
      三浦尚之
    • Organizer
      第17回ウイルス学夏の学校 みちのくウイルス塾
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Industrial Wastewater as a Feedstock for Fermentative Biohydrogen Production Enhanced by Addition of Different Metal Nanoparticles2018

    • Author(s)
      Elreedy, A., Fujii, M, Tawfik A.
    • Organizer
      Water and Environment Technology Conference 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Iron Uptake Kinetics of Bloom-Forming Cyanobacteria2018

    • Author(s)
      Fu, Q.L., Fujii M.
    • Organizer
      Water and Environment Technology Conference 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The Effect of 1,4-Dioxane Inhibition on The Activity, Performance and Recovery on Anaerobic Ammonium Oxidation (Anammox) Process2018

    • Author(s)
      Ismail, S., Elsamadony, M., Fujii, M, Tawfik A.
    • Organizer
      Water and Environment Technology Conference 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Simple Fabrication of Economically-Viable Magnetic Carbon Nanotubes-TiO2 Composite for Degradation of Organic Pollutants2018

    • Author(s)
      Awfa, D., Ateia, M., Fujii, M., Yoshimura, C.
    • Organizer
      IWA World Water Congress, 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Increased toxin production by metal induced oxidative stress for freshwater cyanobacterium Microcystis aeruginosa2018

    • Author(s)
      Fujii, M.
    • Organizer
      6th Australian & New Zealand Cyanobacteria Workshop
    • Related Report
      2018 Annual Research Report
  • [Presentation] Iron Uptake Kinetics of Bloom-Forming Cyanobacteria2018

    • Author(s)
      Fu, Q., L., Fujii, M., Yeung, A. C. Y., and Waite, T. D.
    • Organizer
      6th Australian & New Zealand Cyanobacteria Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 湖沼水質が藍藻毒生成と微生物多様性に及ぼす影響2018

    • Author(s)
      中谷鴻太、藤井
    • Organizer
      第5回環境水質工学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Primary Result of Relationship between Microbial Communities and Water Quality in a Large Tropical Lake2018

    • Author(s)
      Ann, V., Ung, P., Uk, S., Kodikara, D., Siev, S., Peng, C., Yuk, S., Sann, S., Khanal, R., Tan, R., Hul, S., Miyanaga, K., Fujii, M., Yoshimura, C., Tanji, Y.,
    • Organizer
      The 3rd International Symposium on Conservation and Management of Tropical Lakes
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Book] Integrated Coastal Management in the Japanese Satoumi: Restoring Estuaries and Bays2019

    • Author(s)
      Fujii, M., Natsuike, M., Ito, H., Yoshimura C.,
    • Total Pages
      250
    • Publisher
      Elsevier Science Publishing Co Inc.
    • ISBN
      9780128130605
    • Related Report
      2019 Annual Research Report
  • [Book] Health in Ecological Perspectives in the Anthropocene2019

    • Author(s)
      Carvajal, T. M., H. T. Ho, L. F. T. Hernandez, K. M. Viacrusis, D. M. Amalin, and K. Watanabe
    • Total Pages
      159
    • Publisher
      Springer
    • ISBN
      9789811325267
    • Related Report
      2018 Annual Research Report
  • [Book] 第2章開放性内湾の持続可能な利用と海洋環境の実現を目指して 2.3 森は海の恋人か(「里海管理論-きれいで豊かで賑わいのある持続的な海」 柳哲雄編)2019

    • Author(s)
      藤井学、夏池真史、伊藤紘晃、吉村千洋
    • Total Pages
      368
    • Publisher
      農林統計協会
    • ISBN
      9784541042811
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2017-07-21   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi