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Establishment of highly safe gene therapy without usage of double stranded DNA

Research Project

Project/Area Number 17H06269
Research Category

Grant-in-Aid for Challenging Research (Pioneering)

Allocation TypeSingle-year Grants
Research Field General internal medicine and related fields
Research InstitutionHiroshima University

Principal Investigator

Chayama Kazuaki  広島大学, 医系科学研究科(医), 教授 (00211376)

Co-Investigator(Kenkyū-buntansha) 三木 大樹  広島大学, 医系科学研究科(医), 講師 (10584592)
茶山 弘美  広島大学, 医系科学研究科(医), 准教授 (70572329)
林 亮平  広島大学, 病院(医), 病院助教 (80772053)
Project Period (FY) 2017-06-30 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
Fiscal Year 2019: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Keywordsgenome editing / exosome / dsDNA / cancer therapy / lentivirus / cas9 / ゲノム編集
Outline of Final Research Achievements

Gene therapy can be used for many diseases such as hereditary diseases, cancer and viral infections. Many recessive diseases, which account for a large number of inherited diseases, restore the cell function by normalizing one site of the gene. Although gene therapyis a desirable therapy, it has been regarded as difficult to implement because there is no easy method for gene repair. Genes can be normalized by using the recently developed CRISPR / cas9 system, which is expected to make a breakthrough in gene therapy. However, the conventional methods have a risk of causing unnecessary gene modification. In this study, we attempted to perform a safe gene therapy. To perform this we established a method to express cas9 protein in normal hepatocytes.

Academic Significance and Societal Importance of the Research Achievements

本研究は遺伝子治療を安全性に行うという肝炎からの改良を模索したものである。dsDNAを生体に導入することなく、しかもCas9の長期的な非特異切断も防いでいる点で従来の遺伝子治療とは異なっており、Cas9を用いた遺伝子治療の応用を遺伝病のみならず癌やウイルス感染症、代謝性疾患などなど多方面に大きく広げるものとなる。遺伝子治療のもう一つの重要な要因にdeliveryがある。本研究ではそれらのいずれかを応用することを視野に入れつつ、自己の細胞から産生されるexcreted vesicle(EV)をdeliveryに用いることを考案した。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (61 results)

All 2020 2019 2018 2017 Other

All Journal Article (49 results) (of which Int'l Joint Research: 15 results,  Peer Reviewed: 49 results,  Open Access: 48 results) Presentation (6 results) (of which Int'l Joint Research: 1 results) Book (1 results) Remarks (4 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Three Children Treated with Direct-acting Antivirals for Chronic Hepatitis C Virus Genotype 1b Infection2020

    • Author(s)
      Ohya K, Kawaoka T, Imamura M, Morio K, Nakahara T, Murakami E, Yamauchi M, Hiramatsu A, Tsuge M, Aikata H, Chayama K.
    • Journal Title

      Internal Medicine

      Volume: 59 Issue: 7 Pages: 941-944

    • DOI

      10.2169/internalmedicine.3824-19

    • NAID

      130007825289

    • ISSN
      0918-2918, 1349-7235
    • Year and Date
      2020-04-01
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regression of Idiopathic Thrombocytopenic Purpura in a Patient With Eradication of Hepatitis C Virus by Direct-Acting Antivirals.2020

    • Author(s)
      Johira Y, Hatooka H, Mori N, Fukuhara T, Takaki S, Shimomura Y, Imamura M, Hayes CN, Tsuji K, Chayama K.
    • Journal Title

      Hepatology.

      Volume: 71 Issue: 1 Pages: 389-391

    • DOI

      10.1002/hep.30900

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Successful retreatment with 12 weeks of glecaprevir and pibrentasvir for a genotype 2a HCV-infected hemodialysis patient who failed to respond to 8 weeks of prior glecaprevir and pibrentasvir therapy.2020

    • Author(s)
      Ohya K, Imamura M, Osawa M, Teraoka Y, Morio K, Fujino H, Ono A, Nakahara T, Murakami E, Yamauchi M, Kawaoka T, Hiramatsu A, Tsuge M, Aikata H, Hayes CN, Chayama K.
    • Journal Title

      Clin J Gastroenterol.

      Volume: 13 Issue: 2 Pages: 267-270

    • DOI

      10.1007/s12328-019-01039-w

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Management of refractory ascites attenuates muscle mass reduction and improves survival in patients with decompensated cirrhosis.2020

    • Author(s)
      Namba M, Hiramatsu A, Aikata H, Kodama K, Uchikawa S, Ohya K, Morio K, Fujino H, Nakahara T, Murakami E, Yamauchi M, Kawaoka T, Tsuge M, Imamura M, Chayama K.
    • Journal Title

      J Gastroenterol.

      Volume: 55 Issue: 2 Pages: 217-226

    • DOI

      10.1007/s00535-019-01623-4

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Population Pharmacokinetics of Glecaprevir/Pibrentasvir in HCV-infected Japanese Subjects in Phase 3 CERTAIN-1 and CERTAIN-2 Trials.2020

    • Author(s)
      Suleiman AA, Lin CW, Liu W, Eckert D, Mensing S, Burroughs M, Kato K, Chayama K, Kumada H, Oberoi RK.
    • Journal Title

      J Clin Pharmacol.

      Volume: 60 Issue: 3 Pages: 331-339

    • DOI

      10.1002/jcph.1524

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Cost-Effectiveness Analysis of Glecaprevir/Pibrentasvir Versus Existing Direct-Acting Antivirals to Treat Chronic Hepatitis C in Japan.2020

    • Author(s)
      Kawaguchi I, Chayama K, Gonzalez YS, Virabhak S, Mitchell D, Yuen C, Kumada H.
    • Journal Title

      Adv Ther.

      Volume: 37 Issue: 1 Pages: 457-476

    • DOI

      10.1007/s12325-019-01166-3

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Risk factors for histological progression of non-alcoholic steatohepatitis analyzed from repeated biopsy cases.2020

    • Author(s)
      Daijo K, Nakahara T, Inagaki Y, Nanba M, Nishida Y, Uchikawa S, Kodama K, Oya K, Morio K, Fujino H, Ono A, Murakami E, Yamauchi M, Kawaoka T, Miki D, Tsuge M, Hiramatsu A, Hayes CN, Imamura M, Aikata H, Ochi H, Chayama K.
    • Journal Title

      J Gastroenterol Hepatol.

      Volume: - Issue: 8 Pages: 1412-1419

    • DOI

      10.1111/jgh.14968

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Preoperative PET-CT is useful for predicting recurrent extrahepatic metastasis of hepatocellular carcinoma after resection.2020

    • Author(s)
      Morio K, Kawaoka T, Aikata H, Namba M, Uchikawa S, Kodama K, Ohya K, Fujino H, Nakahara T, Murakami E, Yamauchi M, Tsuge M, Hiramatsu A, Imamura M, Nakamura Y, Akagi M, Awai K, Kobayashi T, Ohdan H, Chayama K.
    • Journal Title

      Eur J Radiol.

      Volume: 124 Pages: 108828-108828

    • DOI

      10.1016/j.ejrad.2020.108828

    • NAID

      120007097014

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A case of advanced HCC treated with lenvatinib after hepatic arterial infusion chemotherapy combined with radiation therapy treatment for portal vein tumor thrombosis in the main trunk.2020

    • Author(s)
      Kosaka Y, Kawaoka T, Aikata H, Suehiro Y, Yamaoka K, Ando Y, Namba M, Takeuchi Y, Fujii Y, Uchikawa S, Kodama K, Oya K, Morio K, Fujino H, Nakahara T, Murakami E, Yamauchi M, Tsuge M, Hiramatsu A, Imamura M, Baba Y, Awai K, Kimura T, Nagata Y, Chayama K.
    • Journal Title

      Clin J Gastroenterol.

      Volume: - Issue: 5 Pages: 839-843

    • DOI

      10.1007/s12328-020-01093-9

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complete response to pembrolizumab in advanced hepatocellular carcinoma with microsatellite instability.2020

    • Author(s)
      Ando Y, Yamauchi M, Suehiro Y, Yamaoka K, Kosaka Y, Fuji Y, Uchikawa S, Kodama K, Morio K, Fujino H, Nakahara T, Ono A, Murakami E, Kawaoka T, Takahashi S, Tsuge M, Hiramatsu A, Imamura M, Chayama K, Aikata H.
    • Journal Title

      Clin J Gastroenterol.

      Volume: - Issue: 5 Pages: 867-872

    • DOI

      10.1007/s12328-020-01099-3

    • NAID

      120007167205

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Publisher Correction: Regulation of the Hepatitis B virus replication and gene expression by the multi-functional protein TARDBP.2020

    • Author(s)
      Makokha GN, Abe-Chayama H, Chowdhury S, Hayes CN, Tsuge M, Yoshima T, Ishida Y, Zhang Y, Uchida T, Tateno C, Akiyama R, Chayama K.
    • Journal Title

      Sci Rep.

      Volume: 10 Issue: 1 Pages: 2233-2233

    • DOI

      10.1038/s41598-020-58888-6

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] JNJ-4178 (adafosbuvir, odalasvir, and simeprevir) in Japanese patients with chronic hepatitis C virus genotype 1 or 2 infection with or without compensated cirrhosis: The Phase IIa OMEGA-3 study.2020

    • Author(s)
      Takehara T, Chayama K, Kurosaki M, Yatsuhashi H, Tanaka Y, Hiramatsu N, Sakamoto N, Asahina Y, Nozaki A, Nakano T, Hagiwara Y, Shimizu H, Yoshida H, Huang Y, Biermer M, Vijgen L, Hayashi N.
    • Journal Title

      J Gastroenterol

      Volume: - Issue: 6 Pages: 640-652

    • DOI

      10.1007/s00535-020-01672-0

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Incidence of microsatellite instability-high hepatocellular carcinoma among Japanese patients and response to pembrolizumab.2020

    • Author(s)
      Kawaoka T, Ando Y, Yamauchi M, Suehiro Y, Yamaoka K, Kosaka Y, Fuji Y, Uchikawa S, Morio K, Fujino H, Nakahara T, Ono A, Murakami E, Takahashi S, Tsuge M, Hiramatsu A, Imamura M, Chayama K, Aikata H.
    • Journal Title

      Hepatol Res.

      Volume: - Issue: 7 Pages: 885-888

    • DOI

      10.1111/hepr.13496

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Serum miR-379 expression is related to the development and progression of hypercholesterolemia in non-alcoholic fatty liver disease2020

    • Author(s)
      Okamoto, K. Koda, M. Okamoto, T. Onoyama, T. Miyoshi, K. Kishina, M. Matono, T. Kato, J. Tokunaga, S. Sugihara, T. Hiramatsu, A. Hyogo, H. Tobita, H. Sato, S. Kawanaka, M. Hara, Y. Hino, K. Chayama, K. Murawaki, Y. Isomoto, H.
    • Journal Title

      PLoS One

      Volume: 15 Issue: 2 Pages: e0219412-e0219412

    • DOI

      10.1371/journal.pone.0219412

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Classification of primary liver cancer with immunosuppression mechanisms and correlation with genomic alterations.2020

    • Author(s)
      Fujita M, Yamaguchi R, Hasegawa T, Shimada S, Arihiro K, Hayashi S, Maejima K, Nakano K, Fujimoto A, Ono A, Aikata H, Ueno M, Hayami S, Tanaka H, Miyano S, Yamaue H, Chayama K, Kakimi K, Tanaka S, Imoto S, Nakagawa H.
    • Journal Title

      EBioMedicine

      Volume: 53 Pages: 102659-102659

    • DOI

      10.1016/j.ebiom.2020.102659

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diminished hepatic IFN response following HCV clearance triggers HBV reactivation in coinfection.2020

    • Author(s)
      Cheng X, Uchida T, Xia Y, Umarova R, Liu CJ, Chen PJ, Gaggar A, Suri V, Mucke MM, Vermehren J, Zeuzem S, Teraoka Y, Osawa M, Aikata H, Tsuji K, Mori N, Hige S, Karino Y, Imamura M, Chayama K, Liang TJ.
    • Journal Title

      J Clin Invest.

      Volume: - Issue: 6 Pages: 3205-3220

    • DOI

      10.1172/jci135616

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Targeting clinical epigenetic reprogramming for chemoprevention of metabolic and viral hepatocellular carcinoma.2020

    • Author(s)
      Juhling F, Hamdane N, Crouchet E, Li S, El Saghire H, Mukherji A, Fujiwara N, Oudot MA, Thumann C, Saviano A, Roca Suarez AA, Goto K, Masia R, Sojoodi M, Arora G, Aikata H, Ono A, Tabrizian P, Schwartz M, Polyak SJ, Davidson I, Schmidl C, Chayama K, et al.
    • Journal Title

      Gut.

      Volume: - Issue: 1 Pages: 157-169

    • DOI

      10.1136/gutjnl-2019-318918

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The impact of interferon-free direct-acting antivirals on clinical outcome after curative treatment for hepatitis C virus-associated hepatocellular carcinoma: Comparison with interferon-based therapy.2019

    • Author(s)
      Nagaoki Y, Imamura M, Nishida Y, Daijo K, Teraoka Y, Honda F, Nakamura Y, Morio K, Fujino H, Nakahara T, Kawaoka T, Tsuge M, Hiramatsu A, Kawakami Y, Miki D, Hiyama Y, Ochi H, Chayama K, Aikata H.
    • Journal Title

      J Med Virol.

      Volume: 91 Issue: 4 Pages: 650-658

    • DOI

      10.1002/jmv.25352

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A nationwide survey on non-B, non-C hepatocellular carcinoma in Japan: 2011-2015 update.2019

    • Author(s)
      Tateishi R, Uchino K, Fujiwara N, Takehara T, Okanoue T, Seike M, Yoshiji H, Yatsuhashi H, Shimizu M, Torimura T, Moriyama M, Sakaida I, Okada H, Chiba T, Chuma M, Nakao K, Isomoto H, Sasaki Y, Kaneko S, Masaki T, Chayama K, Koike K.
    • Journal Title

      J Gastroenterol.

      Volume: 54 Issue: 4 Pages: 367-376

    • DOI

      10.1007/s00535-018-1532-5

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] HOMA-IR: An independent predictor of advanced liver fibrosis in nondiabetic non-alcoholic fatty liver disease.2019

    • Author(s)
      Fujii H, Yoneda M, et al.
    • Journal Title

      J Gastroenterol Hepatol.

      Volume: in press Issue: 8 Pages: 1-6

    • DOI

      10.1111/jgh.14595

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Clinical features of hepatocellular carcinoma in nonalcoholic fatty liver disease patients without advanced fibrosis2019

    • Author(s)
      Kodama Kazuhisa、Kawaguchi Takumi、Hyogo Hideyuki、Nakajima Tomoaki、Ono Masafumi、Sumida Yoshio、Kamada Yoshihiro、Fujii Hideki、Seko Yuya、Takehara Tetsuo、Itoh Yoshito、Nakajima Atsushi、Masaki Naohiko、Torimura Takuji、Saibara Toshiji、Karino Yoshiyasu、Chayama Kazuaki、Tokushige Katsutoshi
    • Journal Title

      Journal of Gastroenterology and Hepatology

      Volume: in press Issue: 9 Pages: 1626-1632

    • DOI

      10.1111/jgh.14608

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Percutaneous transvenous shunt occlusion for portosystemic encephalopathy due to lenvatinib administration to a patient with hepatocellular carcinoma and portosystemic shunt.2019

    • Author(s)
      Namba M, Kawaoka T, Aikata H, Kodama K, Uchikawa S, Ohya K, Morio K, Fujino H, Nakahara T, Murakami E, Yamauchi M, Tsuge M, Hiramatsu A, Imamura M, Baba Y, Awai K, Chayama K.
    • Journal Title

      Clin J Gastroenterol.

      Volume: 12 Issue: 4 Pages: 341-346

    • DOI

      10.1007/s12328-019-00938-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparison of intracellular responses between HBV genotype A and C infection in human hepatocyte chimeric mice2019

    • Author(s)
      Tsushima K, Tsuge M, Hiraga N, Uchida T, Murakami E, Makokha GN, Kurihara M, Nomura Motonobu, Hiyama Y, Fujino H, Ono A, Nakahara T, Yamauchi M, Abe-Chayama H, Kawaoka T, Miki D, Imamura M, Aikata H, Hayes CN, Chayama K
    • Journal Title

      Journal of Gastroenterology

      Volume: 印刷中 Issue: 7 Pages: 650-659

    • DOI

      10.1007/s00535-019-01558-w

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reply: Hepatectomy versus stereotactic body radiotherapy for primary early hepatocellular carcinoma: A propensity -matched analysis in a single institution.2019

    • Author(s)
      Nakano R, Ohira M, Kobayashi T, Ide K, Tahara H, Kuroda S, Shimizu S, Kimura T, Nagata Y, Aikata H, Chayama K, Ohdan H.
    • Journal Title

      Surgery.

      Volume: 165 Issue: 5 Pages: 1054-1057

    • DOI

      10.1016/j.surg.2018.10.022

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] HCV-Induced Epigenetic Changes Associated With Liver Cancer Risk Persist After Sustained Virologic Response.2019

    • Author(s)
      Hamdane N, Juhling F, Crouchet E, El Saghire H, Thumann C, Oudot MA, Bandiera S, Saviano A, Ponsolles C, Roca Suarez AA, Li S, Fujiwara N, Ono A, Davidson I, Bardeesy N, Schmidl C, Bock C, Schuster C, Lupberger J, Habersetzer F, Doffoel M, Piardi T, Sommacale D, Imamura M, Chayama K. et al.
    • Journal Title

      Gastroenterology.

      Volume: 156 Issue: 8 Pages: 2313-2329.e7

    • DOI

      10.1053/j.gastro.2019.02.038

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Real-world virological efficacy and safety of daclatasvir/asunaprevir/beclabuvir in patients with chronic hepatitis C virus genotype 1 infection in Japan.2019

    • Author(s)
      Takaguchi K, Toyoda H, Tsutsui A, Suzuki Y, Nakamuta M, Imamura M, Senoh T, Nagano T, Tada T, Tachi Y, Hiraoka A, Michitaka K, Shibata H, Joko K, Okubo H, Tsuji K, Takaki S, Watanabe T, Ogawa C, Chayama K, Kumada T, Kudo M, Kumada H.
    • Journal Title

      J Gastroenterol.

      Volume: 54 Issue: 8 Pages: 742-751

    • DOI

      10.1007/s00535-019-01568-8

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Integrated analysis of 8-week glecaprevir/pibrentasvir in Japanese and overseas patients without cirrhosis and with hepatitis C virus genotype 1 or 2 infection.2019

    • Author(s)
      Naganuma A, Chayama K, Notsumata K, Gane E, Foster GR, Wyles D, Kwo P, Crown E, Bhagat A, Mensa FJ, Otani T, Larsen L, Burroughs M, Kumada H.
    • Journal Title

      J Gastroenterol.

      Volume: 54 Issue: 8 Pages: 752-761

    • DOI

      10.1007/s00535-019-01569-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Combined Analysis of Metabolomes, Proteomes, and Transcriptomes of Hepatitis C Virus-Infected Cells and Liver to Identify Pathways Associated With Disease Development.2019

    • Author(s)
      Lupberger J, Croonenborghs T, Roca Suarez AA, Van Renne N, Juhling F, Oudot MA, Virzi A, Bandiera S, Jamey C, Meszaros G, Brumaru D, Mukherji A, Durand SC, Heydmann L, Verrier ER, El Saghire H, Hamdane N, Bartenschlager R, Fereshetian S, Ramberger E, Sinha R, Nabian M, Chayama K. et al.
    • Journal Title

      Gastroenterology.

      Volume: 157 Issue: 2 Pages: 537-551.e9

    • DOI

      10.1053/j.gastro.2019.04.003

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genomic and Transcriptomic Profiling of Combined Hepatocellular and Intrahepatic Cholangiocarcinoma Reveals Distinct Molecular Subtypes2019

    • Author(s)
      Xue Ruidong、Chen Lu、Zhang Chong、Fujita Masashi et al.
    • Journal Title

      Cancer Cell

      Volume: 35 Issue: 6 Pages: 932-947

    • DOI

      10.1016/j.ccell.2019.04.007

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Hepatitis B virus X protein (HBx) enhances centrosomal P4.1-associated protein (CPAP) expression to promote hepatocarcinogenesis.2019

    • Author(s)
      Yen CJ, Yang ST, Chen RY, Huang W, Chayama K, Lee MH, Yang SJ, Lai HS, Yen HY, Hsiao YW, Wang JM, Lin YJ, Hung LY.
    • Journal Title

      J Biomed Sci.

      Volume: 26 Issue: 1 Pages: 44-44

    • DOI

      10.1186/s12929-019-0534-9

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of the Hepatitis B virus replication and gene expression by the multi-functional protein TARDBP2019

    • Author(s)
      Makokha Grace Naswa、Abe-Chayama Hiromi、Chowdhury Sajeda、Hayes C. Nelson、Tsuge Masataka、Yoshima Tadahiko、Ishida Yuji、Zhang Yizhou、Uchida Takuro、Tateno Chise、Akiyama Rie、Chayama Kazuaki
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 8462-8462

    • DOI

      10.1038/s41598-019-44934-5

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficacy of glecaprevir and pibrentasvir treatment for genotype 1b hepatitis C virus drug resistance-associated variants in humanized mice.2019

    • Author(s)
      Osawa M, Uchida T, Imamura M, Teraoka Y, Fujino H, Nakahara T, Ono A, Murakami E, Kawaoka T, Miki D, Tsuge M, Hiramatsu A, Abe-Chayama H, Hayes CN, Makokha GN, Aikata H, Ishida Y, Tateno C, Miyayama Y, Hijikata M, Chayama K.
    • Journal Title

      J Gen Virol.

      Volume: 100 Issue: 7 Pages: 1123-1131

    • DOI

      10.1099/jgv.0.001268

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Correlation between Early Tumor Marker Response and Imaging Response in Patients with Advanced Hepatocellular Carcinoma Treated with Lenvatinib.2019

    • Author(s)
      Kodama K, Kawaoka T, Namba M, Uchikawa S, Ohya K, Morio K, Nakahara T, Murakami E, Yamauchi M, Hiramatsu A, Imamura M, Chayama K, Aikata H.
    • Journal Title

      Oncology.

      Volume: 97 Issue: 2 Pages: 75-81

    • DOI

      10.1159/000499715

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Management of HCV patients in cases of direct-acting antiviral failure.2019

    • Author(s)
      Hayes CN, Imamura M, Chayama K.
    • Journal Title

      Expert Rev Gastroenterol Hepatol.

      Volume: 13 Issue: 9 Pages: 839-848

    • DOI

      10.1080/17474124.2019.1651642

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Early changes in ammonia levels and liver function in patients with advanced hepatocellular carcinoma treated by lenvatinib therapy.2019

    • Author(s)
      Ohya K, Kawaoka T, Namba M, Uchikawa S, Kodama K, Morio K, Nakahara T, Murakami E, Hiramatsu A, Tsuge M, Yamauchi M, Imamura M, Chayama K, Aikata H.
    • Journal Title

      Sci Rep.

      Volume: 9 Issue: 1 Pages: 12101-12101

    • DOI

      10.1038/s41598-019-48045-z

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficacy of a self-management program in patients with chronic viral hepatitis in China.2019

    • Author(s)
      Cui Y, Moriyama M, Chayama K, Liu Y, Ya C, Muzembo BA, Rahman MM.
    • Journal Title

      BMC Nurs.

      Volume: 18 Issue: 1 Pages: 44-44

    • DOI

      10.1186/s12912-019-0366-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ribavirin induces hepatitis C virus genome mutations in chronic hepatitis patients who failed to respond to prior daclatasvir plus asunaprevir therapy.2019

    • Author(s)
      Saito Y, Imamura M, Uchida T, Osawa M, Teraoka Y, Fujino H, Nakahara T, Ono A, Murakami E, Kawaoka T, Miki D, Tsuge M, Serikawa M, Aikata H, Abe-Chayama H, Hayes CN, Chayama K.
    • Journal Title

      J Med Virol.

      Volume: 92 Issue: 2 Pages: 210-218

    • DOI

      10.1002/jmv.25602

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] eVIDENCE: a practical variant filtering for low-frequency variants detection in cell-free DNA2019

    • Author(s)
      Mizuno Kei、Akamatsu Shusuke、Sumiyoshi Takayuki、Wong Jing Hao、Fujita Masashi et al.
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 15017-15017

    • DOI

      10.1038/s41598-019-51459-4

    • NAID

      120006734882

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pruritus in patients with chronic liver disease and serum autotaxin levels in patients with primary biliary cholangitis.2019

    • Author(s)
      Fujino H, Tanaka M, Imamura M, Morio K, Ono A, Nakahara T, Murakami E, Kawaoka T, Takahashi S, Miki D, Tsuge M, Hiramatsu A, Aikata H, Hayes CN, Chayama K.
    • Journal Title

      BMC Gastroenterol.

      Volume: 19 Issue: 1 Pages: 169-169

    • DOI

      10.1186/s12876-019-1092-z

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Role of Lipids in Hepatocellular Carcinoma.2019

    • Author(s)
      Hayes CN, Zhang P, Chayama K.
    • Journal Title

      Hepatocellular Carcinoma.

      Volume: -

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolated cardiac metastases of hepatocellular carcinoma after resection: a case report.2019

    • Author(s)
      Tamura Y, Kawaoka T, Aikata H, Namba M, Fujii Y, Morio K, Murakami E, Yamauchi M, Hiramatsu A, Nakahara T, Imamura M, Maruhashi T, Kobayashi T, Ohdan H, Kimura T, Nagata Y, Arihiro K, Chayama K.
    • Journal Title

      Clin J Gastroenterol.

      Volume: - Issue: 3 Pages: 421-427

    • DOI

      10.1007/s12328-019-01075-6

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative study on the replication of HCV1b genome between wild-type and cell culture-adaptive mutant in regard to sensitivities against anti-HCV drugs.2019

    • Author(s)
      Miyayama Y, Lee H, Song H, Abe-Chayama H, Miki D, Imamura M, Chayama K, Hijikata M.
    • Journal Title

      Microbiol Immunol.

      Volume: - Issue: 4 Pages: 296-303

    • DOI

      10.1111/1348-0421.12768

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sinusoidal obstruction syndrome post-treatment with trastuzumab emtansine (T-DM1) in advanced breast cancer.2019

    • Author(s)
      Fujii Y, Doi M, Tsukiyama N, Hattori Y, Ohya K, Shiroma N, Morio K, Morioka T, Aikata H, Shinozaki K, Chayama K.
    • Journal Title

      Int Cancer Conf J.

      Volume: 9 Issue: 1 Pages: 18-23

    • DOI

      10.1007/s13691-019-00392-4

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of HBV integration patterns and timing in liver cancer and HBV-infected livers.2018

    • Author(s)
      Furuta M, Tanaka H, Shiraishi Y, Unida T, Imamura M, Fujimoto A, Fujita M, Sasaki-Oku A, Maejima K, Nakano K, Kawakami Y, Arihiro K, Aikata H, Ueno M, Hayami S, Ariizumi SI, Yamamoto M, Gotoh K, Ohdan H, Yamaue H, Miyano S, Chayama K, Nakagawa H.
    • Journal Title

      Oncotarget.

      Volume: 9 Issue: 38 Pages: 25075-25088

    • DOI

      10.18632/oncotarget.25308

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genomic Characterization of Biliary Tract Cancers Identifies Driver Genes and Predisposing Mutations2018

    • Author(s)
      Wardell CP, Fujita M, Yamada T, Simbolo M, Fassan M, Karlic R, Polak P, Kim J, Hatanaka Y, Maejima K, Lawlor RT, Nakanishi Y, Mitsuhashi T et al
    • Journal Title

      J Hepatol

      Volume: - Issue: 5 Pages: 959-969

    • DOI

      10.1016/j.jhep.2018.01.009

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] miR-135a-5p-mediated downregulation of protein tyrosine phosphatase receptor delta is a candidate driver of HCV-associated hepatocarcinogenesis.2018

    • Author(s)
      Van Renne N, Roca Suarez AA, Duong FHT, Gondeau C, Calabrese D, Fontaine N, Ababsa A, Bandiera S, Croonenborghs T, Pochet N, De Blasi V, Pessaux P, Piardi T, Sommacale D, Ono A, Chayama K, Fujita M, Nakagawa H, Hoshida Y, Zeisel MB, Heim MH, Baumert TF, Lupberger J.
    • Journal Title

      Gut.

      Volume: 67 Issue: 5 Pages: 953-962

    • DOI

      10.1136/gutjnl-2016-312270

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Serial profiling of circulating tumor DNA for optimization of anti-VEGF chemotherapy in metastatic colorectal cancer patients.2018

    • Author(s)
      Yamauchi M, Urabe Y, Ono A, Miki D, Ochi H, Chayama K.
    • Journal Title

      Int J Cancer.

      Volume: 142 Issue: 7 Pages: 1418-1426

    • DOI

      10.1002/ijc.31154

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Silencing of Discoidin Domain Receptor-1 (DDR1) Concurrently Inhibits Multiple Steps of Metastasis Cascade in Gastric Cancer2017

    • Author(s)
      Yuge R, Kitadai Y, Takigawa H, Naito T, Oue N, Yasui W, Tanaka S, Chayama K
    • Journal Title

      Transl Oncol

      Volume: 11 Issue: 3 Pages: 575-584

    • DOI

      10.1016/j.tranon.2018.02.003

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mesenchymal Stem Cells Induce Epithelial to Mesenchymal Transition in Colon Cancer Cells through Direct Cell-to-Cell Contact.2017

    • Author(s)
      Takigawa H, Kitadai Y, Shinagawa K, Yuge R, Higashi Y, Tanaka S, Yasui W, Chayama K.
    • Journal Title

      Neoplasia.

      Volume: 19 Issue: 5 Pages: 429-438

    • DOI

      10.1016/j.neo.2017.02.010

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] HCV治療の今後の展望-ウイルス性肝炎撲滅を目指した最新治療2019

    • Author(s)
      茶山一彰
    • Organizer
      第45回日本急性肝不全研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] HCV治療の今後の展望-ウイルス性肝炎撲滅を目指した最新治療2019

    • Author(s)
      茶山一彰
    • Organizer
      第20回日本肝がん分子標的治療研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 最新のC型肝炎、肝硬変の治療2019

    • Author(s)
      茶山一彰
    • Organizer
      第33回日本臨床内科医学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ウイルス性肝炎研究の進歩と今後の課題2019

    • Author(s)
      茶山一彰
    • Organizer
      第23回日本肝臓学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 肝硬変診療の課題2019

    • Author(s)
      茶山一彰
    • Organizer
      日本消化器関連学会機構(JDDW)2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] Malignant potential of early-stage serrated adenocarcinoma based on genetic analysis2018

    • Author(s)
      Urabe Y, Tanaka S, Hirano D, Nakamura K, Ninomiya Y, Yuge R, Hayashi R, Oka S, Kitadai Y, Shimamoto F, Arihiro K, Chayama K
    • Organizer
      26th United European Gastroenterology Week (UEGW) 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Book] 肝原発平滑筋肉腫の一例2019

    • Author(s)
      山岡賢治, 児玉健一郎, 河岡友和, 難波麻衣子, 内川慎介, 大屋一輝, 盛生 慶, 中原隆志, 村上英介, 山内理海, 平松 憲, 今村道雄, 相方 浩, 織田麻琴, 有廣光司, 小林 剛, 大段秀樹, 茶山一彰
    • Publisher
      肝臓
    • Related Report
      2019 Annual Research Report
  • [Remarks] 広島大学大学院 医系科学研究科 消化器・代謝内科学

    • URL

      http://shounai.hiroshima-u.ac.jp/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 広島大学 自立型研究拠点 肝臓・消化器研究拠点

    • URL

      http://shounai.hiroshima-u.ac.jp/liverproject/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 広島大学大学院医系科学研究科 消化器・代謝内科(広島大学病院 旧第一内科)

    • URL

      http://shounai.hiroshima-u.ac.jp/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 広島大学大学院医歯薬保健学研究科 消化器・代謝内科(広島大学病院 旧第一内科)

    • URL

      http://shounai.hiroshima-u.ac.jp/

    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] B型肝炎治療用核酸分子2019

    • Inventor(s)
      茶山一彰/吉間忠彦/水谷隆之
    • Industrial Property Rights Holder
      茶山一彰/吉間忠彦/水谷隆之
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-514692
    • Filing Date
      2019
    • Acquisition Date
      2019
    • Related Report
      2019 Annual Research Report

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Published: 2017-07-21   Modified: 2021-02-19  

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