Impact of microRNAs in the exosomes secreted from adipocytes on diet-induced prostate cancer development
Project/Area Number |
17H06535
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Urology
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Research Institution | Akita University |
Principal Investigator |
Nara Taketoshi 秋田大学, 医学部附属病院, 助教 (00801888)
|
Project Period (FY) |
2017-08-25 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 前立腺癌 / 高脂肪食 / エクソソーム / microRNA |
Outline of Final Research Achievements |
Here we assessed the impact of microRNAs in the exosomes secreted from adipocytes on diet-induced prostate cancer initiation and development using in vitro model, in vivo model, and human tissues. Our previous models have several limitations such as immune deficient mice, different diet sources, and ectopic models. In this study, we successfully developed two different immunocompetent mouse models of lard diet-induced prostate cancer progression. Moreover, we extracted exosomes from 22Rv-1 prostate cancer cells and human adipose tissues from surgical specimens in cancer patients and confirmed size, concentration and marker expression of these exosomes.
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Academic Significance and Societal Importance of the Research Achievements |
高脂肪食や肥満は前立腺癌の危険因子といわれている。また、脂肪細胞は過剰に摂取したエネルギー貯蔵庫としての機能だけでなく、エクソソームなどを分泌する内分泌細胞としての機能を有することが明らかとなっている。さらにエクソソーム内のmicroRNAと癌化の関連が様々な癌種で報告されている。本研究では、高脂肪食摂取および肥満下の前立腺癌発症・進展と脂肪組織、エクソソーム、microRNAの関与を検討した。
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Report
(3 results)
Research Products
(5 results)
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[Journal Article] Impact of Nuclear YAP1 Expression in Residual Cancer After Neoadjuvant Chemohormonal Therapy With Docetaxel for High-Risk Localized Prostate Cancer2020
Author(s)
Yoshinori Matsuda, Shintaro Narita, Taketoshi Nara, Huang Mingguo, Hiromi Sato, Atsushi Koizumi, Sohei Kanda, Kazuyuki Numakura, Mitsuru Saito, Takamitsu Inoue, Yuko Hiroshima, Hiroshi Nanji, Shigeru Satoh, Norihiko Tsuchiya, Tomonori Habuchi
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Journal Title
BMC Cancer
Volume: 20
Issue: 1
Pages: 2034-2034
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] The impact of specific fat diets and obesity on prostate cancer initiation and progression using two differen t immunocompetent mouse models2020
Author(s)
Hiromi Sato , Shintaro Narita , Ryohei Yamamoto , Atsushi Koizumi , Taketoshi Nara , Sohei Kanda , Kazuyuki Numakura Mitsuru Saito , Takamitsu Inoue , Shigeru Satoh , Toshiaki Yosh ioka , Tomonori Habuchi .
Organizer
EAU 2020, vertual.
Related Report
Int'l Joint Research
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[Presentation] HFD leads to metabolic modification of prostate cancer promoting stroma by the upregulation of MIC-12019
Author(s)
Huang, M., Narita, S., Koizumi, A., Nara, T., Numakura, K., Satoh, S., Nanjo, H., Habuchi, T.
Organizer
第 78 回日本癌学会学術総会
Related Report
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[Presentation] Macrophage inhibitory cytokine-1 stimulates stromal cytokine production in high-fat diet-induced prostate cancer progression.2018
Author(s)
Huang, M., Narita, S., Koizumi, A., Nara, T. ,Kanda, S., Numakura, K., Tsuruta, H., Saito, M.,Inoue, T. and Habuchi, T.
Organizer
The 113th Annual Meeting of the American Urological Association
Related Report
Int'l Joint Research