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Novel regulatory mechanism of endochondral ossification via CCN2-VASH1 axis

Research Project

Project/Area Number 17H06885
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionOkayama University

Principal Investigator

MURASE Yurika  岡山大学, 大学病院, 医員 (70803708)

Research Collaborator TAKIGAWA Masaharu  岡山大学, 医歯薬学総合研究科, 教授 (20112063)
SATO Yasufumi  東北大学, 加齢医学研究所, 教授 (50178779)
KUBOTA Satoshi  岡山大学, 医歯薬学総合研究科, 教授 (90221936)
AOYAMA Eriko  岡山大学, 医歯薬学総合研究科, 助教 (10432650)
SUZUKI Yasuhiro  東北大学, 加齢医学研究所, 助教 (60332277)
HATTORI Takako  岡山大学, 医歯薬学総合研究科, 助教 (00228488)
YOSHIDA Shoko  岡山大学, 大学病院, 医員 (00616047)
SASAKI Akira  岡山大学, 医歯薬学総合研究科, 教授 (00170663)
Project Period (FY) 2017-08-25 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsCCN2 / VASH1 / 内軟骨性骨化 / 軟骨
Outline of Final Research Achievements

The aim of this study is to investigate a novel regulatory mechanism of endochondral ossification by CCN2 and VASH1. We found that both CCN2 and VASH1 were localized in the hypertrophic chondrocyte layer. CCN2-silencing in chondrocytes reduced the expression of VASH1 and increased apoptotic cells, and its increase was suppressed by a ROS inhibitor, N-acetyl-L-cysteine. These results suggest that up-regulation of CCN2-VASH1 axis may suppress the elevation of ROS level that causes chondrocyte cell death/apoptosis and keep hypertrophic chondrocytes surviving until the terminal stage of chondrogenic differentiation.

Academic Significance and Societal Importance of the Research Achievements

学術的意義:本研究により、未だにほとんど解明されていない「内軟骨性骨化の最終段階―軟骨から骨への転化段階」の調節メカニズムの一端を解明する成果が生み出された。
社会的意義:本研究により、骨・軟骨形成に異常をきたす疾病の原因・病態究明、治療法開発につながる成果が生み出された。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • Research Products

    (6 results)

All 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results)

  • [Journal Article] Physiological role of urothelial cancer-associated one long noncoding RNA in human skeletogenic cell differentiation2017

    • Author(s)
      Ishikawa T, Nishida T, Ono M, Takarada T, Nguyen HT, Kurihara S, Furumatsu T, Murase Y, Takigawa M, Oohashi T, Kamioka H, Kubota S
    • Journal Title

      J Cell. Physiol.

      Volume: 233(6) Issue: 6 Pages: 4825-4840

    • DOI

      10.1002/jcp.26285

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] CCN2-VASH1-SOD2 axisによる軟骨細胞終末分化における細胞死の制御2018

    • Author(s)
      村瀬友里香,青山絵理子,鈴木康弘,佐々木 朗,久保田 聡,佐藤靖史,滝川正春
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] CCN2-VASH1-SOD2 axisを介した内軟骨性骨化調節機構2018

    • Author(s)
      村瀬友里香,青山絵理子,鈴木康弘,佐々木 朗,久保田 聡,佐藤靖史,滝川正春
    • Organizer
      第31回日本軟骨代謝学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Vasohibin-1 (VASH1)による内軟骨性骨化の制御とCCN2の関与2017

    • Author(s)
      村瀬友里香,青山絵理子,久保田 聡,佐々木 朗,滝川正春
    • Organizer
      第35回日本骨代謝学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 内軟骨性骨化過程におけるvasohibin-1 (VASH1)の発現とその意義2017

    • Author(s)
      村瀬友里香,吉田祥子,佐々木 朗
    • Organizer
      第62回(公社)日本口腔外科学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Expression of vasohibin-1 (VASH1) during endochondral ossification and its significance2017

    • Author(s)
      村瀬友里香,青山絵理子,鈴木康弘,佐々木 朗,久保田 聡,佐藤靖史,滝川正春
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report

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Published: 2017-08-25   Modified: 2020-03-30  

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