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Identifying and functional analysis of axial stem cell-specific gene

Research Project

Project/Area Number 17H06905
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionThe University of Tokushima

Principal Investigator

HAYASHI Shinichi  徳島大学, 先端酵素学研究所(オープンイノベ), 特任助教 (80599572)

Project Period (FY) 2017-08-25 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords体軸幹細胞 / Wnt3a / Tbx6 / Greb1 / 体軸形成 / T/Sox2 / RNA-Seq / 多能性 / 沿軸中胚葉
Outline of Final Research Achievements

To identify regulatory factor for axial stem cells, we performed transcriptome analysis in Wnt3a knockout and Tbx6 knockout mouse. As a result, we found 6 genes as candidates of axial stem cell regulatory factors. We especially focused on transcription regulator, Greb1 which is induced by estrogen receptor was expressed in the caudal region of embryo where axial stem cells reside.
We made Greb1 knockout mouse by genome editing using CRISPR/Cas9. As a result, shortened body axis was observed suggesting that Greb1 involved in regulation of axial stem cells. This result gives us an insight to dissect molecular mechanism of axial stem cell regulation.

Academic Significance and Societal Importance of the Research Achievements

マウスの胚発生における体軸幹細胞の発見は三胚葉説に再考を促す重要な知見であり、教科書が書き換えられたほど反響が大きかった。体軸幹細胞の存在が2011年に示されてから、体軸幹細胞の研究報告は増加している。しかし、多能性や維持機構という重要な本質の解明は未だ成されていない。本研究において、体軸幹細胞の制御因子の候補が見つかったことは体軸幹細胞の多能性と維持機構を明らかにする上で重要な一歩であり、ひいては我々のボディプランを解き明かすことに繋がる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • Research Products

    (2 results)

All 2019 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Chromatin dynamics underlying the precise regeneration of a vertebrate limb -epigenetic regulation and cellular memory.2019

    • Author(s)
      Shinichi Hayashi, Koji Tamura, Hitoshi Yokoyama.
    • Journal Title

      Seminars in Cell and Developmental Biology.

      Volume: 印刷中

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Sall4 regulates mesoderm development from neuro-mesodermal progenitors during the body elongation of mouse embryo.2017

    • Author(s)
      Shinichi Hayashi
    • Organizer
      第50回 日本発生生物学会大会
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-08-25   Modified: 2024-12-25  

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