• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the mechanism integrating light and circadian clock signals, which control rice flowering time

Research Project

Project/Area Number 17H07356
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Science in genetics and breeding
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Ogo Yuko  国立研究開発法人農業・食品産業技術総合研究機構, 次世代作物開発研究センター, 主任研究員 (90572214)

Project Period (FY) 2017-08-25 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsイネ / 出穂 / 開花 / 光 / 発現制御 / 転写因子 / ゲノム編集 / 転写制御 / 光応答 / 概日時計
Outline of Final Research Achievements

In rice, a short- day plant, floral transition in response to environmental conditions have been extensively studied. The molecular cascade Grain number, plant height, and heading date 7 (Ghd7)→Early heading date 1→Heading date 3a/Rice FT-like 1 and its interaction with Heading date 1 plays an important role for floral transition of rice. These genes are intricately regulated by light and circadian clocks.
In this study, we analyzed the transcriptional regulation of Ghd7 by light and circadian clocks. We produced the transformant rice for analysis of transcriptional regulation of Ghd7. Using these transformant rice, the expression pattern of Ghd7 and the downstream genes were analyzed in various day length conditions. The transformant rice with reporter genes after Ghd7 locus were produced by the efficient agrobacterium-mediated gene targeting system. ChIP-seq analysis was performed by transformant rice with the phytochrome-GFP protein.

Academic Significance and Societal Importance of the Research Achievements

短日植物のイネの厳格な日長応答は、長日植物のシロイヌナズナとは異なるものであり、その制御機構について多くの研究がなされてきた。しかしながら、その中で重要な働きを示すGhd7について、発現制御機構の解析は進んでいなかった。本研究では、高効率遺伝子組換え法などの様々な新しい手法を用いて、Ghd7がどのように光と概日時計により制御されているのかを解析した。また、Ghd7は、開花期だけでなく植物体の生育にも大きな影響を与えることを再確認した。今後、どのようにGhd7が重要農業形質に関わっているのかを調べる予定であり、農業現場への応用にも重要であると考えられる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report

URL: 

Published: 2017-08-25   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi