| Project/Area Number |
17J02728
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Research Field |
Ecology/Environment
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
MARS BRISBIN Margaret (2018-2019) 沖縄科学技術大学院大学, 科学技術研究科, 特別研究員(DC1)
MARS BRISBIN Margaret Anne (2017) 沖縄科学技術大学院大学, 科学技術研究科, 特別研究員(DC1)
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| Project Period (FY) |
2017-04-26 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2019: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2018: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2017: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Radiolaria / symbiosis / Phaeocystis / Acantharea / Plankton / Protists |
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| Outline of Annual Research Achievements |
During the fiscal year 2019 I completed my PhD thesis and thesis defense. I presented my final thesis chapter as a poster at the Ocean Sciences Meeting in California. In addition, I submitted 3 first author manuscripts for publication; they are under review and are openly available as preprints on bioRxiv. The chapter investigated gene expression in symbiotic Phaeocystis (Haptyophyta) while living inside Acantharian (Rhizaria) hosts. I performed single-holobiont RNA-seq with individual acantharian hosts. I then performed RNA-seq with 3 biological replicates each from two cultured Phaeocystis species, which I used to create reference transcriptomes and map symbiont transcripts from the holobionts. I then performed differential expression analyses for the two symbiont species. Results showed that symbionts upregulated photosynthesis and gene expression, but down regulated DNA replication and cell cycle progression. Importantly, symbiont gene expression did not suggest that symbionts were at all nutrient limited, which is a common method hosts use to limit symbiont cell division. Instead, hosts must be deploying a nutrient independent control mechanism.
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| Research Progress Status |
令和元年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和元年度が最終年度であるため、記入しない。
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