| Project/Area Number |
17J04057
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Research Field |
Environmental agriculture(including landscape science)
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| Research Institution | Tohoku University |
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Principal Investigator |
FERNANDES SIQUEIRA Arthur (2018) 東北大学, 生命科学研究科, 特別研究員(PD)
FERNANDES SIQUEI ARTHUR (2017) 東北大学, 生命科学研究科, 特別研究員(DC2)
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| Project Period (FY) |
2017-04-26 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2018: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2017: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Bradyrhizobium / Denitrification / Anaerobiosis / Competitiveness / cbb3 oxidase / Bacterial Physiology |
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| Outline of Annual Research Achievements |
In the present research, co-culture experiments showed that B. japonicum is less competitive than B. diazoefficiens under low oxigen contidions. Also the heam staining of NapC and immunoblotting analysis of NapA indicated lower levels of Nap in B. japonicum than in B. diazoefficiens under low-oxygen conditions, in the presence of nitrate, as a possible reason for the disadvantage of B. japonicum. Also, the lower production of FixP and FixO protein (part of cbb3-type high-affinity cytochrome oxidase) may be related to the inability of B. japonicum to produce Nap in sufficient levels. These results support my previous observation that nitrate reduction capacity may be an important factor driving the distribution of bradyrhizobia in response to soil oxygen conditions.
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| Research Progress Status |
平成30年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
平成30年度が最終年度であるため、記入しない。
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