Monitoring of PPCPs in STP effluent and evaluation of their potencies to alter susceptibility to infectious disease in fish

• Project/Area Number: 17K00583
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental impact assessment
• Research Institution: Ehime University
• Principal Investigator: Nakayama Kei 愛媛大学, 沿岸環境科学研究センター, 講師 (80380286)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 免疫毒性 / 医薬品 / 下水処理水 / コイ / Aeromonas salmonicida / 環境 / 影響評価手法 / 細菌感染症

Outline of Final Research Achievements

In the present study, we have monitored 81 compounds of pharmaceuticals and personal care products (PPCPs) in effluent of a sewage treatment plant through a whole year, and have identified some certain PPCPs such as NSAIDs, anti-ulcer agents, and antihistamic agents, whose levels in the effluents are relatively high. We also optimized the method of experimental infection test using common carp (Cyprinus carpio) as a host and Aeromonas salmonicida as a pathogen. We fixed the temperature for culturing the bacteria at 20 degrees C, and decided to conduct the infection test around 23 dgrees C. Additionally, size-dependent alteration of susceptibility in carp against A. salmonicida was evaluated. We found that body size of carp unaffected the susceptibility, when we use the fish whose body weight are between 1.6 to 8.0 g. Using the fixed experimental conditions, immunomodulately effects of chemicals will be evaluated.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果により，生態毒性の観点からの免疫毒性の評価において，従来法のような免疫刺激の無い条件下で化学物質の影響を解析するのではなく，病原体に対する感受性の変化を評価する試験が可能となった。近年，当該分野における国際学会においても免疫毒性を主題としたセッションが継続的に開かれており，とくに本テーマに関する研究は高い関心を集めている。野生生物における感染症の発生に対して化学物質の関与が疑われる事例があるものの，未だその証明には至っておらず，本研究で提案する手法が免疫毒性評価の推進に大きく貢献出来ると考えている。

Research Products

• [Presentation] コイとAeromonas salmonicidaを用いた免疫毒性評価法の構築 (2020)
  • Author(s): 仲山 慶，北村真一
  • Organizer: 令和2年度日本魚病学会春季大会
• [Presentation] Optimization of the infection test method using common carp with Aeromonas salmonicida for the assessment of immunotoxicity (2019)
  • Author(s): Nakayama K, Yasumoto S, Kitamura SI
  • Organizer: SETAC North America 40th Annual Meeting
  • Int'l Joint Research
• [Presentation] Proposed minimum requirements for the immunotoxicity test to assess chemically induced alteration of susceptibility to pathogen in fish (2018)
  • Author(s): Nakayama K and Kitamura SI
  • Organizer: SETAC North America 39th Annual Meeting
  • Int'l Joint Research
• [Presentation] 病原体に対する感受性の変化をエンドポイントとした免疫毒性の評価～最近の動向～ (2018)
  • Author(s): 仲山 慶，北村真一
  • Organizer: 第24回日本環境毒性学会研究発表会
• [Presentation] Determination of suitable host-pathogen combinations to detect the chemical effects on susceptibility to infectious disease (2017)
  • Author(s): Nakayama K and Kitamura SI
  • Organizer: SETAC North America 38th Annual Meeting
  • Int'l Joint Research
• [Remarks] 魚類免疫毒性評価
  • URL: http://www.team-fische.com/bio/?cat=48