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Osteological and genetic analysis of Jamainu specimens from the point of view of the crossbreed events between Japanese wolves and domestic dogs

Research Project

Project/Area Number 17K01193
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cultural assets study and museology
Research InstitutionThe Graduate University for Advanced Studies

Principal Investigator

Ishiguro Naotaka  総合研究大学院大学, 先導科学研究科, 客員研究員 (00109521)

Co-Investigator(Kenkyū-buntansha) 本郷 一美  総合研究大学院大学, 先導科学研究科, 准教授 (20303919)
寺井 洋平  総合研究大学院大学, 先導科学研究科, 助教 (30432016)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsニホンオオカミ / イヌ / ミトコンドリア / DNA / 系統解析 / ミトコンドリアDNA / ゲノム解析 / ヤマイヌ / 二ホンオオカミ / オオカミ / ゲノム / DNA
Outline of Final Research Achievements

Japanese wolves (Canis lupus hodophilax, Temminck, 1839) has been called as Ookami or Jamainu. We examined mitochondrial DNA (mtDNA) sequences from five Japanese wolf specimens found in Japan, three specimens from Museum fur Naturkunde in Berlin and three specimens (Jentink a, Jentink b and Jentink c) in Siebold collection of Naturalis Biodiversity Center in Leiden. The mitochondrial genome of the type specimen of hodophilax (Jentink c) called as Jamainu in the Siebold collection was included in the cluster of Japanese wolves. Although mitochondrial lineage of Jentink c is Japanese wolf, the morphological features and the size of Jentink c specimen are different from those of other Japanese wolves indicates that the crossbreed events between Japanese wolves and domestic dogs might be occurred in the past.

Academic Significance and Societal Importance of the Research Achievements

シーボルトが蒐集した標本を含めて海外の博物館に保管されているニホンオオカミの標本のmtDNA解析は、本研究が初めてである。これまでシーボルトコレクションのヤマイヌの正体は不明であったが、本研究によりヤマイヌはニホンオオカミ型のmtDNAを有することが明らかとなった。これにより、当時ヤマイヌと呼ばれていた動物は、ニホンオオカミかあるいはイヌとの交雑種であることが示された。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2020 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (4 results) (of which Invited: 3 results)

  • [Journal Article] Analysis of the Mitochondrial Genomes of Japanese Wolf Specimens in the Siebold Collection, Leiden2020

    • Author(s)
      Matsumura Shuichi、Terai Yohey、Hongo Hitomi、Ishiguro Naotaka
    • Journal Title

      Zoological Science

      Volume: 38 Issue: 1 Pages: 60-66

    • DOI

      10.2108/zs200019

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Computed tomography examination and mitochondrial DNA analysis of Japanese wolf skull covered with skin2017

    • Author(s)
      Naotaka Ishiguro, Yasuo Inoshima and Motoki Sasaki
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 79 Issue: 1 Pages: 14-17

    • DOI

      10.1292/jvms.16-0429

    • NAID

      130005289878

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] ニホンオオカミ(Canis lupus hodophilax)頭蓋内部構造の定量的および形態学的解析2019

    • Author(s)
      鈴木千尋、佐々木基樹、都築直、茅野光範、石黒直隆、山田一考、遠藤秀紀、菊池智景、菊池薫、北村延夫
    • Organizer
      日本獣医学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 謎につつまれた動物 -二ホンオオカミー2019

    • Author(s)
      石黒直隆
    • Organizer
      第23回紀伊半島研究会シンポジウム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] ニホンオオカミの古代ゲノム2018

    • Author(s)
      石黒 直隆
    • Organizer
      日本遺伝学会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 絶滅した動物ーニホンオオカミの謎ー2018

    • Author(s)
      石黒直隆
    • Organizer
      日本獣医師会
    • Related Report
      2017 Research-status Report
    • Invited

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Published: 2017-04-28   Modified: 2022-01-27  

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