Application of BirA enzyme-labeling methods for the discovery of health food ingredients to prevent muscle atrophy
| Project/Area Number |
17K01847
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Kobe University |
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Principal Investigator |
Ueda Shuji 神戸大学, 農学研究科, 助教 (50379400)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 筋肉 / ヒートショック蛋白質 / BIOID2 / 老化 / 運動 / 健康機能性食品 / BioID2 / HSPBs / 国際情報交換 / 動物性食品 / 健康食品成分 / BirA / BioID / RhoA |
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| Outline of Final Research Achievements |
In this study, based on the BioID enzyme labeling technique using a biotin ligase mutant (BirA), we searched for essential molecules involved in muscle atrophy via heat shock proteins (HSPBs). As a result, we succeeded in identifying several molecules that are included in the cytoprotective function of HSPBs to suppress muscle atrophy caused by endoplasmic reticulum stress. This result suggested cellular stress protection via the Polo-like kinase 2 (PLK2)/HSPB5 pathway in the endoplasmic reticulum stress response of muscle cells.
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| Academic Significance and Societal Importance of the Research Achievements |
高齢者では、筋力及び筋肉量が低下し、持続的な運動負荷により改善が期待される。しかし、高齢者の効果的な筋萎縮予防の実践には、過度な運動負荷による身体への負担が懸念される。本研究では、HSPBsに着目し、筋萎縮の抑制に効果的なHSPBsの機能を明らかにした。今後、HSPBsの発現増加に着目した栄養成分を探索することで、過度な運動負荷に頼らない予防法の考案に繋がることが期待される。
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Report
(3 results)
Research Products
(11 results)
