Multidimensional investigation of Glycopeptide-lectin interaction
| Project/Area Number |
17K05920
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
Hinou Hiroshi 北海道大学, 先端生命科学研究院, 教授 (70333333)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | レクチン / 糖ペプチド / 相互作用 / 配座 / マイクロアレイ / NMR / O-マンノース / ムチン / 糖鎖 / 植物レクチン / ガレクチン / 動物レクチン / 蛍光ラベル / glycopeptide / lectin / microarray / interaction / conformation |
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| Outline of Final Research Achievements |
Regarding the interaction between glycopeptides and lectins, mucin-type glycopeptides and O-mannose-type glycopeptides were used as models for interaction analysis by microarray and conformational analysis by NMR. In mucin-type glycopeptides, hydrogen bonds and hydrophobic interactions between glycans and peptides are strongly involved in conformations of both sugar chains and peptides. Both of the glycan and peptide moiety is markedly restricted near the binding sites of the glycan on the peptides. It was found that the binding property of lectin changes with the conformational change. On the other hand, it was confirmed that the O-mannose type glycopeptide does not cause conformational restriction of glycan, but that the conformational restriction of peptides occurs with the addition of a specific sugar residue. Corresponding to this peptide conformational change, the NMR signal and lectin binding pattern also altered.
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| Academic Significance and Societal Importance of the Research Achievements |
糖鎖によるタンパク質の翻訳後修飾は生物学的に不可欠であるが、純粋な特定構造の調整が困難であるため、糖ペプチドレベルでの構造活性相関研究が遅れている。本研究では糖鎖認識に関わるレクチンと糖鎖の相互作用に焦点を当て、糖ペプチドレベルでの認識能の変化に関する研究を世界で初めて系統的に実施した。この成果は単なるたんぱく質の認識現象の理解の促進にとどまらず、疾患や生命システムの理解に貢献することが期待される。また、感染症においても糖鎖-レクチン（ヘマグルチニン）間の相互作用は宿主識別の主要機能であることから感染症の宿主変化の理解においても理解の促進に貢献すると思われる。
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] The use of fluoroproline in MUC1 antigen enables efficient detection of antibodies in patients with prostate cancer2017
Author(s)
Somovilla V.J., Bermeijo I.A., Albuquerque I.S., Martinez S. N., Castro Lopez J., Garcia Martin F., Companon I., Hinou H., Nishimura S-I., Jimenez Barbero J., Asensio J.L, Avenoza G., Busto J.H., Hurtado-Guerrero. R., Peregrina J.M., Bernardes G.J.L., Corzana F.
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Journal Title
J. Am. Chem. Soc.
Volume: 129
Issue: 50
Pages: 18255-18261
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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