Low-cost degradation of azo dyes using the artifitial multi-domain enzymes immobilized on the sheet.
| Project/Area Number |
17K05936
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
永田 崇 京都大学, エネルギー理工学研究所, 准教授 (10415250)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | アゾレダクターゼ / アゾ色素 / タンパク質工学 / アフィニティークロマトグラフィー / 固定化酵素 / カードラン / GRP-tag / ハイブリッド酵素 / 水質改善 / 融合タンパク質 / プロテインライゲーション / 蛋白質 / 酵素反応 / バイオテクノロジー / 分子機械 / 環境技術 |
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| Outline of Final Research Achievements |
Azoreductase is NADPH-dependent enzyme that cleaves azo bond in azo dyes. Since NADPH is a high-cost compound, we have sequentially connected an NADPH regeneration system with glucokinase and glucose 6-phosphate dehydrogenase domains to obtain a hybrid azoreductase. The degradation efficiency of azo dyes with the hybrid azoreductase was higher when the domains were connected via flexible linkers. In order to understand the mechanisms of how the linkers contribute to increasing degradation rate of azo dyes, we will track the enzymatic reaction of each domain in the hybrid enzymes using the real-time NMR method.
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| Academic Significance and Societal Importance of the Research Achievements |
代表的な合成染料であるアゾ色素は、生産コストが低いことから、さまざまな繊維の染色において大量利用されている。アゾ色素はその高い化学的安定性により、廃棄にあたっては、凝集沈殿や加温処理などの高いエネルギーコストを必要とする。
本研究では、NADPH再生酵素群をアゾレダクターゼと組み合わせ、より少量の補酵素でアゾ色素を連続分解できるハイブリッド酵素を開発した。このハイブリッド酵素をカードランシートに低コストで固定化することで、工業的な大規模利用への端緒を開いた。また、ハイブリッド酵素による複雑な補酵素再生およびアゾ色素の分解カスケードを、NMR法により精密かつ効率的に観測することにも成功した。
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Report
(5 results)
Research Products
(16 results)
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[Journal Article] Specific Inhibition of FGF5-induced Cell Proliferation by RNA Aptamers2021
Author(s)
Amano, R., Namekata, M., Horiuchi, M., Saso, M., Yanagisawa, T., Tanaka, Y., Ghani, F.I., Yamamoto, M., Sakamoto T.
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Journal Title
Scientific Reports
Volume: 11
Issue: 1
Pages: 2976-2976
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] NMR analysis for the development of artificial RNAs to control biomolecular function.2020
Author(s)
Kumagai, K., Suzuki, T., Sekikawa, Y., Kamba, K, Wan, L., Nagata, K., Takaori-Kondo, A., Katahira, M., Nagata, T., Sakamoto, T.
Organizer
The 11th International Symposium of Advanced Energy Science
Related Report
Int'l Joint Research
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[Presentation] Real-time NMR analysis of the continuous degradation process of azo dyes using azoreductase in cooperation with the NADPH regeneration system.2019
Author(s)
Horiuchi, M., Nagata, T., Katahira, M., Kobashigawa, Y., Suzuki, Y., Ochiai, M.
Organizer
The 9th International Symposium of Advanced Energy Science
Related Report
Int'l Joint Research
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[Presentation] Development of the artificial multi-domain enzymes immobilized on the curdlan sheet at low cost.2017
Author(s)
Horiuchi, M., Nagata, T., Katahira, M., Kobashigawa, Y., Suzuki, Y., Ochiai, M.
Organizer
The 8th International Symposium of Advanced Energy Science
Related Report
Int'l Joint Research
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