| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Outline of Final Research Achievements |
We examined to prepare a highly active glycosyltransferase for the functionalization of biopharmaceuticals by modification of N-glycan. As the results, it was found that N-glycosylated human FUT8 mutants have higher activities than the wild type enzyme. In addition, the molecular assembly of FUT8 was investigated, to reveal the mechanism of activation of FUT8 enzyme. It was suggested that human FUT8 potentially forms homodimer via intermolecular hydrophobic interactions involving the α-helical and SH3 domains associated with the catalytic domain.
On the basis of these results, we would like to design more active FUT8 mutants for the development of N-glycan-modified biopharmaceuticals.
|
