| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Pollen transmission of raspberry bushy dwarf virus mutant (RBdel1bstop) to healthy plants was significantly decreased compared with that of the wild-type raspberry bushy dwarf virus (wtRBDV). When we assessed accumulation of viral genomic (g) RNAs in pollen grains from RBdel1bstop-infected plants, the pollen grains had less viral gRNAs than in those from wtRBDV-infected plants. In addition, pollen grains from 1b-expressing transgenic plants (1b-plants) infected with RBdel1bstop transmitted the mutant virus to healthy plants after pollination more efficiently than did pollen from RBdel1bstop-infected wild-type plants (wt-plants), and more viral gRNAs had accumulated in pollen grains from RBdel1bstop-infected 1b-plants than in pollen from RBdel1bstop-infected infected wt-plants. We suggest that 1b promotes discharge of the viral gRNAs from the germinating pollen grains. Furthermore, we found that the 1b had RNA silencing suppressor activity.
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