| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
To increase the cellulase production in Trichoderma reesei, we tried to adjust the expression levels of sopohose producing and degrading enzymes and sugar-transporter which involved in cellobiose uptake. The increase of Cel1A expression resulted in increase of the cellulase production. However, the remarkable increase of Cel1A caused the decrease of cellulase production. These suggested that the suitable control of Cel1A expression might be important for increase of the cellulase production. On the other hand, it was found that Cel3B deletion mutant produced cellulase twice as compared to that of the parent strain.
Moreover, the Crt1 overexpression mutant produced the cellulase 1.7 times higher. From the results of the overexpression and the deletion mutants of Crt1, it was firstly discovered that Crt1 was essential to expression of Cel1A and the transcription factor Xyr1.
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