| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Ionic liquids have physical properties for use as reaction solvents. To solve the problems of growth inhibition of microorganisms by ionic liquids, we isolated the ionic liquid-tolerant Bacillus sp. CMW1. The first objective of this study is to identify the gene which are responsible for ionic-liquid tolerant. The second objective is to develop the novel bacterial host which exhibits tolerance to ionic liquids.
Consequently, I identified MFS transporter gene from strain CMW1 and showed that the gene is responsible for an ionic-liquid tolerant. The gene of CMW1 was transformed into Brevibacillus sp. bacterium and the recombinant strain could grow in the presence of an ionic liquid. Using tetracycline, pHY300PLK was successfully electroporated into strain CMW1 with the efficiency. I obtained the mutants which were able to grow in the presence of hydrophobic ionic liquids. The results will provide a basis for the future work in biocatalysis with various ionic liquids in industry.
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