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Three-dimensional image comprehensive analysis of the proteins produced in the Japanese birch plantlet infected with a canker-rot fungus

Research Project

Project/Area Number 17K07833
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Forest science
Research InstitutionUtsunomiya University

Principal Investigator

Yokota Shinso  宇都宮大学, 農学部, 教授 (60210613)

Co-Investigator(Kenkyū-buntansha) 上高原 浩  京都大学, 農学研究科, 教授 (10293911)
鈴木 智大  宇都宮大学, バイオサイエンス教育研究センター, 准教授 (10649601)
吉永 新  京都大学, 農学研究科, 准教授 (60273489)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsMALDIイメージング / シラカンバ / カバノアナタケ / プロテオーム解析 / 菌感染特異的タンパク質 / ゲノム解析 / 縮合型タンニン / RNA-Seq / プロテオーム / タンパク質 / 樹木病理
Outline of Final Research Achievements

Proteome analysis was performed for the infection specific proteins produced in leaf and petiole, and callus from Japanese birch plantlet No.8 infected with a canker-rot fungus Inonotus obliquus strain IO-U1. As the results, it was revealed that fungal-infection responses are induced for photosynthesis, energy production, detoxification, secondary cell wall formation, PR-10 proteins synthesis, and etc. in the organ and the cells.
Although MALDI imaging analysis was performed for the sections prepared from the infected internodes, specific images for condensed tannins were not obtained. In addition, the whole genome and mitochondrial genome were sequenced for Inonotus obliquus strain IO-B2. As the results, this fungus was found to be closely related to Sanghuangporus sanghuang belonging to Agaricomycetes.

Academic Significance and Societal Importance of the Research Achievements

シラカンバのカンバ類の癌腫病菌カバノアナタケに対する菌感染応答機構を、分子レベルである程度明らかにすることができた。この研究成果は、シラカンバ樹木の病原菌に対する防護方法の開発に応用できるのみならず、他の木本性植物についても適用できる可能性がある。
カバノアナタケの全ゲノム及びミトコンドリア・ゲノムの塩基配列を解読することが出来た。これらの成果は、この菌の病原性及びシラカンバ樹木との相互応答機構の解明に大きく寄与する。また、この菌の菌核(チャーガ)は、抗腫瘍活性等の様々な薬理活性化合物を有しており、これらの化合物の生合成機構の解明等、医学・薬学分野への貢献も期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2020 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] The complete mitochondrial genome sequence of the medicinal fungus Inonotus obliquus (Hymenochaetaceae, Basidiomycota)2019

    • Author(s)
      Agnestisia, R., Ono, A., Nakamura, L., Chino, R., Nodera, K., Aiso-Sanada, H., Nezu, I., Ishiguri, F., Suzuki, T., Yokota, S.
    • Journal Title

      Mitochondrial DNA Part B.

      Volume: 4 Issue: 2 Pages: 3504-3506

    • DOI

      10.1080/23802359.2019.1675548

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Wood-degradation-related genes from genome sequence of fungus Inonotus obliquus strain IO-B2 (NBRC 113408)2020

    • Author(s)
      Retno Agnestisia, Tomohiro Suzuki, Kaito Nodera, Haruna Aiso-Sanada, Luna Nakamura, Futoshi Ishiguri, Shinso Yokota
    • Organizer
      第70回日本木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] カンバ類癌腫病菌カバノアナタケIO-B2株の木材化学成分分解関連遺伝子2020

    • Author(s)
      アグネスティシア・レトノ、小野晶子、中村瑠奈、千野怜、野寺開斗、相蘇春菜、根津郁実、石栗太、鈴木智大、横田信三
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Genome sequence of fungus Inonotus obliquus strain IO-B2 (NBRC 113408) reveals insights into wood degradation2019

    • Author(s)
      Retno Agnestisia, Rei Chino, Kaito Nodera, Haruna Aiso-Sanada, Nezu Ikumi, Tomohiro Suzuki, Futoshi Ishiguri, Shinso Yokota
    • Organizer
      1st International Liginin Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Two-dimensional electrophoresis analysis of infection-specific proteins produced in the callus of Japanese birch plantlet No.8 infected with a canker-rot fungus Inonotus obliquus strain IO-U1 (NBRC 113406)2019

    • Author(s)
      Retno Agnestisia, Rei Chino, Kaito Nodera, Futoshi Ishiguri, Shinso Yokota
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 癌腫病菌カバノアナタケIO-U1株を接種したシラカンバ幼植物体No.8の菌感染部位に生成する特異的タンパク質のプロテオーム解析2018

    • Author(s)
      千野怜、野寺開斗、石栗太、大島潤一、横田信三
    • Organizer
      第36回日本植物細胞分子生物学会(金沢)大会
    • Related Report
      2018 Research-status Report
  • [Presentation] カバノアナタケ菌IO-U1株に感染したシラカンバ幼植物体No.8の葉・葉柄に生成する特異的タンパク質のプロテオーム解析2018

    • Author(s)
      野寺開斗、千野 怜、石栗 太、大島潤一、横田信三
    • Organizer
      第68回日本木材学会大会(京都)
    • Related Report
      2017 Research-status Report
  • [Presentation] カバノアナタケ菌IO-U1株に感染したシラカンバ幼植物体No.8の根における菌感染応答2018

    • Author(s)
      千野 怜、野寺開斗、石栗 太、大島潤一、横田信三
    • Organizer
      第68回日本木材学会大会(京都)
    • Related Report
      2017 Research-status Report
  • [Presentation] 癌腫病菌カバノアナタケIO-U1株に感染したシラカンバ幼植物体No.8の主茎に生成する特異的タンパク質のプロテオーム解析2017

    • Author(s)
      千野 怜、野寺開斗、大島潤一、石栗 太、横田信三
    • Organizer
      第35回日本植物細胞分子生物学会(さいたま)大会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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