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Profiling of ray parenchymal cell-specific gene expression to elucidate heartwood formation mechanism

Research Project

Project/Area Number 17K07878
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Wood science
Research InstitutionKyushu University

Principal Investigator

Jun Shigeto  九州大学, 大学病院, 助教 (70570852)

Co-Investigator(Kenkyū-buntansha) 中川 敏法 (中川敏法)  滋賀県立大学, 環境科学部, 講師 (00734261)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords心材形成 / 心材 / 放射柔細胞 / 遺伝子発現解析 / 代謝物解析
Outline of Final Research Achievements

Recently, genetic research has elucidated the biosynthesis mechanism of tree components, mainly cellulose and lignin, and attempts have been made to artificially control the quality and quantity by genetic modification. On the other hand, little is known about the molecular mechanism that controls the formation of heartwood. Information on gene expression and changes of components in the radial parenchyma cells where heartwood components are synthesized, is essential for elucidating the mechanism of heartwood formation control. In this study, we investigated method of sample preparation for comprehensive gene expression and metabolite analysis, targeting the radial parenchyma cells involved in heartwood formation.

Academic Significance and Societal Importance of the Research Achievements

心材の形成は樹木の特徴であり、特に心材の美しい色と高い耐腐朽性は木材利用にとって極めて重要な考慮すべき特性である。例えばスギでは黒色を呈する異常材が現れ、その林業上の価値は極めて低いものとなってしまう。これらを遺伝的に改良するには、その原因物質(セイクリンC、アガサレジノールなどの重合物)の動態および、生合成される分子メカニズムを知る必要がある。本研究は、“特定の放射柔細胞”を用いた“網羅的な転写解析”および“代謝物解析”に、“時間軸”を加えた動的解析を特徴としており、心材形成(様々な心材成分の生合成・細胞死・物質移動)の分子メカニズムを解明する突破口となり得る。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2019 2018

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results) Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] PRX2 and PRX25 , peroxidases regulated by COG1, are involved in seed longevity in Arabidopsis2019

    • Author(s)
      Renard J, Martinez-Almonacid I, Sonntag A, Molina I, Moya-Cuevas J, Bissoli G, Munoz-Bertomeu J, Faus I, Ninoles R, Shigeto J, Tsutsumi Y, Gadea J, Serrano R, Bueso E.
    • Journal Title

      Plant, Cell & Environment

      Volume: 43 Issue: 2 Pages: 315-326

    • DOI

      10.1111/pce.13656

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Generation of lignin polymer models via dehydrogenative polymerization of coniferyl alcohol and syringyl alcohol via several plant peroxidases involved in lignification and analysis of the resulting DHPs by MALDI-TOF analysis2018

    • Author(s)
      Jun Shigeto, Hiroki Honjo, Koki Fujita, Yuji Tsutsumi
    • Journal Title

      Holzforschung

      Volume: 72 Issue: 4 Pages: 267-274

    • DOI

      10.1515/hf-2017-0125

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Generation of lignin polymer models via dehydrogenative polymerization of coniferyl alcohol and sinapyl alcohol via plant peroxydase involved in lignification and analysis of the resulting DHPs by MALDI-TOF analysis2018

    • Author(s)
      Tsutsumi Y, Shigeto J, Honjo
    • Organizer
      The XXIX International Conference on Polyphenols (ICP) and the 9th Tannin Conference (TC
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] The contribution of CWPO-C to primary stage of plant growth and organogenesis2018

    • Author(s)
      Yoshikay D, Shigeto J, Yokoyama Y, TsutsumiY
    • Organizer
      LignoBiotech 5th Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] CWPO-Cリコンビナントタンパクを用いたシナピルアルコール類縁体の脱水素重合2018

    • Author(s)
      本庄裕貴,重藤潤,堤祐司
    • Organizer
      第68回日本木材学会大会(京都大会)
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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