| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Previously, we found that intravenous injection of chondroitin sulfate (CS), followed by intravenous injection of cationic liposome/siRNA complex (siRNA lipoplex) could deliver siRNA into the liver metastasis. In this study, we examined the effects of the type of cationic lipid in the liposome on siRNA delivery into the liver and liver metastasis. We prepared 17 types of cationic liposomes to evaluate the gene-silencing effect of siRNA in mouse liver following sequential injection of CS plus siRNA lipoplex. In 14 types of cationic liposomes, siRNA was accumulated in the liver when siRNA lipoplexes were injected immediately after CS. However, suppression of targeted mRNA expression in the liver after sequential injection of CS plus siRNA lipoplex were observed in only 3 types of cationic liposomes. Furthermore, sequential injection of PKL1 siRNA or HSF1 siRNA lipoplexes into mice with liver HeLa metastasis could suppress tumor growth.
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