3D morphological processes of slit diaphragm development and regeneration in podocytes

• Project/Area Number: 17K08521
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Juntendo University
• Principal Investigator: Ichimura Koichiro 順天堂大学, 医学部, 准教授 (10343485)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ポドサイト / 足細胞 / 足突起消失 / ネフローゼ症候群 / FIB-SEM / 3D超微形態解析 / スリット膜 / FIB/SEM / 三次元超微形態解析 / 解剖学

Outline of Final Research Achievements

The slit diaphragms of podocytes function as a glomerular filtration barrier to prevent leakage of plasma proteins into the urine. The slit diaphragm is formed during podocyte development and disappears and regenerates during pathologic condition. These three morphological processes of the slit diaphragm alteration are only fragmentarily understood. In this study, we utilized volume electron microscopy (FIB/SEM tomography) to fully elucidate the 3D morphological processes of slit film formation, loss, and regeneration.

Academic Significance and Societal Importance of the Research Achievements

本研究により、これまで断片的にしか分かっていなかったスリット膜の形態変化を完全に解明できた。これらの成果は糸球体疾患の病態解明や診断を研究するうえで基礎的なデータとなる。さらに、FIB-SEMトモグラフィーによるポドサイト解析に関する詳細なプロトコル論文も数編出版でき、他のポドサイト研究者も本手法を活用できるようになった。

Research Products

• [Journal Article] Three-dimensional imaging of podocyte ultrastructure using FE-SEM and FIB/SEM tomography (2020)
  • Author(s): Miyaki T, Kawasaki Y, Hosoyamada Y, Amari T, Kinoshita M, Matsuda H, Kakuta S, Sakai T, Ichimura K.
  • Journal Title: Cell Tissue Res Volume: 379 Issue: 2 Pages: 245-254
  • DOI: 10.1007/s00441-019-03118-3
  • NAID: 120006883563
  • Peer Reviewed / Open Access
• [Journal Article] Volume Scanning Electron Microscopy for 3D Analysis of Biological Ultrastructure (2020)
  • Author(s): Miyaki T, Kawasaki Y, Hosoyamada Y, Amari T, Kinoshita M, Matsuda H, Kakuta S, Sakai T, Ichimura K.
  • Journal Title: Juntendo Medical Journal Volume: 66 Issue: 2 Pages: 108-119
  • DOI: 10.14789/jmj.2020.66.JMJ19-R19
  • NAID: 130007838166
  • ISSN: 2187-9737, 2188-2126
  • Peer Reviewed / Open Access
• [Journal Article] Anatomic characterization of the femoral nutrient artery: application to fracture and surgery of the femur (2020)
  • Author(s): Kawasaki Y, Kinose S, Kato K, Sakai T, Ichimura K.
  • Journal Title: Clin Anat Volume: 33 Issue: 4 Pages: 479-487
  • DOI: 10.1002/ca.23390
  • Peer Reviewed / Open Access
• [Journal Article] Three-dimensional architecture of pericardial nephrocytes in Drosophila melanogaster revealed by FIB/SEM tomography (2019)
  • Author(s): Kawasaki Y, Matsumoto A, Miyaki T, Kinoshita M, Kakuta S, Sakai T, Ichimura K
  • Journal Title: Cell and Tissue Research Volume: 378 Issue: 2 Pages: 289-300
  • DOI: 10.1007/s00441-019-03037-3
  • NAID: 120006883562
  • Peer Reviewed / Open Access
• [Journal Article] Morphological processes of foot process effacement in puromycin aminonucleoside nephrosis revealed by FIB/SEM tomography. (2019)
  • Author(s): Ichimura K, Miyaki T, Kawasaki Y, Kinoshita M, Kakuta S, Sakai T.
  • Journal Title: J Am Soc Nephrol Volume: 30(1) Issue: 1 Pages: 96-108
  • DOI: 10.1681/asn.2018020139
  • NAID: 120006883561
  • Peer Reviewed / Open Access
• [Journal Article] Anatomic characterization of radial and ulnar nutrient arteries in humans. (2018)
  • Author(s): Kinose S, Kanaya Y, Kawasaki Y, Okamura T, Kato K, Sakai T, Ichimura K.
  • Journal Title: Ann Anat Volume: 216 Pages: 23-28
  • DOI: 10.1016/j.aanat.2017.10.004
  • Peer Reviewed
• [Journal Article] Anatomic characterization of the humeral nutrient artery: application to fracture and surgery of the humerus. (2017)
  • Author(s): Ichimura K, Kinose S, Kawasaki Y, Okamura T, Kato K, Sakai T.
  • Journal Title: Clin Anat Volume: 30(7) Issue: 7 Pages: 978-987
  • DOI: 10.1002/ca.22976
  • Peer Reviewed
• [Remarks] プレスリリース：腎疾患における糸球体障害の形態変化の全過程を明らかに
  • URL: https://www.juntendo.ac.jp/news/20190128-01.html
• [Remarks] 解剖学・生体構造科学講座における研究：3D超微形態解析
  • URL: https://jun-anatomy.cambria.ac/research/3s/