| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
It has recently become clear that central nervous system synaptic transmission is regulated by nanometer-scale molecular assemblies (nanoassemblies) formed by synaptic molecules. In this study, we developed a live cell super-resolution imaging technique that can visualize and analyze the spatiotemporal dynamics of nanoassembly of synaptic molecules. By developing a fluorescence probe technology that has characteristics suitable for super-resolution microscopy using the single molecule localization method, and further optimizing the optical system of the microscope system, super-resolution imaging was successfully performed in living cells. The live cell super-resolution imaging technology developed in this research can be expected to be applied not only to neuroscience but also to biological research in various fields.
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