| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We screened regulatory factors for somatic imprinted DMR establishment within Igf2r/Airn domain using shRNA libraries. We found six genes as candidates. Second-order analyses revealed two genes as putative regulatory factors for the somatic DMR establishment. In addition, we generated model mice, in which candidate regions for DMR establishment were deleted, to clarify a mechanism of DMR establishment within Kcnq1ot1/Cdkn1c domain. Since DMR methylation did not change in the model mice, it was suggested that the regulatory region for DMR establishment did not exist within the deleted region. However, it was suggested that at least the enhancer for Cdkn1c would be included within the deleted region because Cdkn1c expression decreased in the model mice.
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