Analysis of the gut microbe-derived molecular pattern recognized by the serum IgG of ulcerative colitis patients

• Project/Area Number: 17K08833
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Kagawa University
• Principal Investigator: KUWAHARARA TOMOMI 香川大学, 医学部, 教授 (60263810)
• Co-Investigator(Kenkyū-buntansha): 橋本 雅仁 鹿児島大学, 理工学域工学系, 教授 (30333537) ; 石川 秀樹 京都府立医科大学, 医学(系)研究科(研究院), 特任教授 (30351795) ; 今大路 治之 (中山治之) 香川大学, 医学部, 講師 (80294669)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 潰瘍性大腸炎 / 腸内細菌 / IgG / IgG結合細菌 / 腸内細菌科細菌 / Bacteroides / 菌叢解析 / Prevotella / 表層分子 / Protein G / 免疫沈降

Outline of Final Research Achievements

Ulcerative colitis (UC) is one of the inflammatory bowel diseases characterized by frequent bloody diarrhea and abdominal pain. Most of the cases recurrent the symptoms and are refractory to medical treatment. In this study, we found that serum IgG of UC patients strongly reacts to Gram-negative enteric bacteria such as Proteus vulgaris and Bacteroides dorei.

Academic Significance and Societal Importance of the Research Achievements

潰瘍性大腸炎において過剰な免疫反応を引き起こしている腸内菌を同定した。今後、IgG抗体の認識抗原の同定を進めることにより、潰瘍性大腸炎の発症に腸内細菌叢がどのように関与しているのかを明らかにすることが可能となる。本研究成果は潰瘍性大腸炎の予後の予測のための診断マーカーや新規治療法の開発に貢献するものと考える。

Research Products

• [Journal Article] Effect of thymoquinone on Fusobacterium nucleatum-associated biofilm and inflammation (2020)
  • Author(s): Tada A, Nakayama-Imaohji H, Yamasaki H, Miad E, Nagao T, Yagi H, Ishikawa M, Shibuya K, Kuwahara T
  • Journal Title: Mol Med Rep Volume: -
  • Peer Reviewed / Open Access
• [Journal Article] Partially hydrolyzed guar gum alleviates small intestinal mucosal damage after massive small bowel resection along with changes in the intestinal microbiota (2019)
  • Author(s): Fujii Takayuki、Chiba Yoichi、Nakayama-Imaohji Haruyuki、Onishi Shun、Tanaka Aya、Katami Hiroto、Kaji Tatsuru、Ieiri Satoshi、Miki Takanori、Ueno Masaki、Kuwahara Tomomi、Shimono Ryuichi
  • Journal Title: Journal of Pediatric Surgery Volume: 54 Issue: 12 Pages: 2514-2519
  • DOI: 10.1016/j.jpedsurg.2019.08.048
  • Peer Reviewed / Open Access
• [Journal Article] An enhanced green fluorescence protein (EGFP)-based reporter assay for quantitative detection of sporulation in Clostridium perfringens SM101. (2019)
  • Author(s): Wakabayashi Y, Nariya H, Yasugi M, Kuwahara T, Sarker MR, Miyake M.
  • Journal Title: Int J Food Microbiol Volume: 291 Pages: 144-150
  • DOI: 10.1016/j.ijfoodmicro.2018.11.015
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Genetic diversity and persistent colonization of Enterococcus faecalis on ocular surfaces (2018)
  • Author(s): Todokoro D, Eguchi H, Suzuki T, Suzuki M, Nakayama-Imaohji H, Kuwahara T, Nomura T, Tomita H, Akiyama H.
  • Journal Title: Jpn J Ophthalmol. Volume: 62 Issue: 6 Pages: 699-705
  • DOI: 10.1007/s10384-018-0630-5
  • Peer Reviewed
• [Journal Article] IgA regulates the composition and metabolic function of gut microbiota by promoting symbiosis between bacteria. (2018)
  • Author(s): Nakajima A, Vogelzang A, Maruya M, Miyajima M, Murata M, Son A, Kuwahara T, Tsuruyama T, Yamada S, Matsuura M, Nakase H, Peterson DA, Fagarasan S, Suzuki K.
  • Journal Title: J Exp Med. Volume: 215 Issue: 8 Pages: 2019-2034
  • DOI: 10.1084/jem.20180427
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of a recombinant Bacteroides fragilis sialidase expressed in Escherichia coli (2018)
  • Author(s): Yamamoto Takaaki、Ugai Hideyo、Nakayama-Imaohji Haruyuki、Tada Ayano、Elahi Miad、Houchi Hitoshi、Kuwahara Tomomi
  • Journal Title: Anaerobe Volume: 50 Pages: 69-75
  • DOI: 10.1016/j.anaerobe.2018.02.003
  • Peer Reviewed
• [Journal Article] L-histidine augments the oxidative damage against Gram-negative bacteria by hydrogen peroxide. (2018)
  • Author(s): Tamiko Nagao，Haruyuki Nakayama-Imaohji，Miad Elahi，Ayano Tada，Emika Toyonaga，Hisashi Yamasaki，Katsuichiro Okazaki，Hirokazu Miyoshi，Koichiro Tsuchiya，Tomomi Kuwahara.
  • Journal Title: International Journal of Molecular Medicine Volume: 41 Pages: 2847-2854
  • DOI: 10.3892/ijmm.2018.3473
  • Peer Reviewed / Open Access
• [Journal Article] Modulatory influence of segmented filamentous bacteria on transcriptomic response of gnotobiotic mice exposed to TCDD. (2017)
  • Author(s): Stedtfeld RD, Chai B, Crawford RB, Stedtfeld TM, Williams MR, Xiangwen S, Kuwahara T, Cole JR, Kaminski NE, Tiedje JM, Hashsham SA.
  • Journal Title: Front Microbiol Volume: 8 Pages: 1708-1708
  • DOI: 10.3389/fmicb.2017.01708
  • Int'l Joint Research
• [Journal Article] TLR2-stimulating contaminants in glycoconjugate fractons prepared from Bacteroides fragilis. (2017)
  • Author(s): Hashimoto M, Waki J, Nakayama-Imaohji H, Ozono M, Hashiguchi S, Kuwahara T.
  • Journal Title: Innate Immun Volume: 23 Issue: 5 Pages: 449-458
  • DOI: 10.1177/1753425917714313
  • Peer Reviewed
• [Journal Article] Nutritional benefit of recycling bowel content in an infant with short bowel syndrome. (2017)
  • Author(s): Tanaka A, Nakayama-Imaohji H, Shimono R, Suzuki M, Fujii T, Kubo H, Yasuda S, Koyano K, Nakamura S, Katsuki N, Kuwahara T.
  • Journal Title: J Pediatr Gastroenterol Nutr Volume: 65 Issue: 3 Pages: 75-76
  • DOI: 10.1097/mpg.0000000000001630
  • Peer Reviewed / Open Access
• [Presentation] 酪酸によるヒト大腸癌細胞の分化誘導とアポトーシスに対する腸 内細菌の影響 (2020)
  • Author(s): 多田 彩乃，今大路 治之，桑原 知巳
  • Organizer: 第94回日本細菌学会総会
• [Presentation] 抗菌薬暴露後に変動する乳幼児腸内フローラの代謝機能 (2020)
  • Author(s): 今大路 治之，田中 彩，下野 隆一，豊田 敦，高見 英人，桑原 知巳
  • Organizer: 第94回日本細菌学会総会
• [Presentation] 知っておきたい腸内細菌の基礎知識 (2018)
  • Author(s): 桑原知巳
  • Organizer: 2018 年度ＮＲ・サプリメントアドバイザーレベルアップセミナー
  • Invited
• [Presentation] 機能メタゲノム解析から見た抗菌薬投与前後 の乳幼児腸内フローラ (2018)
  • Author(s): 今大路治之、田中彩、下野隆一、豊田敦、髙見英人、桑原知巳
  • Organizer: 第91回日本細菌学会総会
• [Presentation] OUTER MEMBRANE VESICLE PRODUCTION IN BACTEROIDES FRAGILIS IS REGULATED BY DNA INVERSION (2017)
  • Author(s): Haruyuki Nakayama-Imaohji, Tomomi Kuwahara
  • Organizer: FEMS 2017
  • Int'l Joint Research